Different tolerance mechanism to alkaline stresses between Populus bolleana and its desert relative Populus euphratica
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Background and aims
Populus bolleana Lauche. (P. bolleana) and Populus euphratica Oliv. (P. euphratica) separately survive in mild and moderate alkaline soil conditions. The aim of this study was to explore the underlying mechanism for the different alkaline tolerance in the two poplar species.
Young saplings of two poplar species were grown in moderate alkaline soil, and the young and old leaves of the two poplars were separately analyzed by ion concentration, allocation and distribution, transcript variation of different genes involved in ion transport and nitrogen assimilation, nitrogen metabolism, organic acid, leaf pigments, and redox responses.
Excess Na+ under alkali stress was mainly allocated to old leaves in P. bolleana. However, excess Na+ was allocated to both young and old leaves in P. euphratica, and was balanced by enhanced levels of Mg2+, Ca2+, and SO42−, with no change in oxidative parameter. The reduction of nitrate nitrogen occurred under alkali stress in both species; P. euphratica acclimated to alkali stress by more flexible regulation of N metabolism and nitrate absorption than P. bolleana.
Our results strongly indicated different alkali tolerance mechanisms in P. bolleana and P. euphratica. P. bolleana protects young tissues via profound accumulation of Na+ and confining damage effects into the old leaves under alkali stress, while P. euphratica can effectively compartmentalize excess Na+, keep its ion balance, and adjust nitrogen transport and metabolism in both young and old leaves to avoid alkali damage.
KeywordsAlkali stress Physiological indexes Nitrate nitrogen Ammonia nitrogen Young and old leaves Alkaline tolerance
Salt overly sensitive
Nicotinamide adenine dinucleotide
NADH-dependent glutamine-2-oxoglutarate aminotransferase
Ferredoxin-dependent glutamate synthase
High-affinity potassium transporter
High-affinity potassium transporter
High performance liquid chromatography
Qualitatively real-time PCR
Reactive oxygen species
Thiobarbituric acid reactive substance
Least significance difference
Tricarboxylic acid cycle
This research was supported by the Youth Foundation of Science and Technology in Sichuan, China, (No. 2014JQ0016), Natural Science Foundation of China (31770644 and 31270660), Project of Innovation research team in the Sichuan Education Administration (No. 13TD0023), and the Longshan Talent Program of Southwestern University of Science and Technology.
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