Plant species-specificity and effects of bioinoculants and fertilization on plant performance for nickel phytomining
- 208 Downloads
To investigate the effects of fertilization and bacterial inoculation on the growth, health and Ni phytoextraction capacity of three Ni-hyperaccumulators, Odontarrhena bracteata, O. inflata and O. serpyllifolia.
Plants were grown for three months in serpentine soil fertilized with inorganic NPK or amended with cow manure and inoculated with five rhizobacterial strains (previously isolated from O. serpyllifolia). Shoot and root dry weight (DW) yields, Ni accumulation and removal, nutritive status and stress indicators were determined.
Plants grown in manure-amended soil showed significantly higher DW yields, improved nutritive status and higher total Ni phytoextracted. Some bacterial inoculants enhanced Ni removal due to the stimulation in growth and/or increase in shoot Ni concentration but this depended on the plant species, soil type and inoculant. Pseudoarthrobacter oxydans strain SBA82 enhanced shoot DW yield of all three Odontarrhena spp. in at least one soil type. Paenarthrobacter sp. strain LA44 and Stenotrophomonas sp. strain MA98 promoted growth of O. serpyllifolia and O. bracteata. Inoculated plants showing growth promotion presented lower activities of antioxidative enzymes, and concentrations of malondialdehyde (MDA) and H2O2, indicating a protective effect of these inoculants on the plants.
Rhizobacterial inoculants applied in combination with manure can improve plant growth and health, and Ni phytoextraction, in some hyperaccumulating Odontarrhena spp.
KeywordsPlant growth promoting rhizobacteria (PGPR) Ultramafic soil Alyssum hyperaccumulators Antioxidant stress enzymes
ZG would like to acknowledge a scholarship from the Ministry of Science, Research and Technology of Iran (MSRT), Graduate School of University of Isfahan and Plant Antioxidants Center of Excellence (PACE) of University of Isfahan. This research was funded by the FACCE Surplus project Agronickel (ID71) and Ministerio de Economía, Industria y Competitividad (PCIN-2017-028). Finally, the authors thank Marián de Jesús González and Lucia Debernardo Espiñeira for technical assistance.
- Benizri E, Kidd PS (2018) The role of the rhizosphere and microbes associated with hyperaccumulator plants in metal accumulation. In: Van der Ent A, Echevarria G, Baker A, Morel JL (eds) Agromining: Farming for Metals. Extracting Unconventional Resources Using Plants. Springer, Berlin, pp 157–188Google Scholar
- Brooks RR (1987) Serpentine and its vegetation: a multidisciplinary approach. Dioscorides Press, PortlandGoogle Scholar
- Busse HJ (2016) Review of the taxonomy of the genus Arthrobacter, emendation of the genus Arthrobacter sensu lato, proposal to reclassify selected species of the genus Arthrobacter in the novel genera Glutamicibacter gen. nov., Paeniglutamicibacter gen. nov., Pseudoglutamicibacter gen. nov., Paenarthrobacter gen. nov. and Pseudarthrobacter gen. nov., and emended description of Arthrobacter roseus. Int J Syst Evol Microbiol 66:9–37CrossRefPubMedGoogle Scholar
- Chaney RL, Angle JS, Baker AJM, Li Y-M (1998) Method for phytomining of nickel, cobalt and other metals from soil. US Patent 5,711,784, 27 Jan 1998Google Scholar
- Chaney RL, Reeves RD et al (2014) Phytoremediation and phytomining: using plants to remediate contaminated or mineralized environments. In: Rajakaruna R, Boyd RS, Harris T (eds) Plant ecology and evolution in harsh environments. Nova Sience Publishers, New York, pp 365–391Google Scholar
- Cuypers A et al (2010) Cadmium stress: an oxidative challenge. Bio Metals 23:927–940Google Scholar
- Fravel D (2005) Commercialization and implementation of biocontrol 1. Annu Rev Phytopathol 43:337–359Google Scholar
- Janmohammadi M, Bihamta M, Ghasemzadeh F (2013) Influence of rhizobacteria inoculation and lead stress on the physiological and biochemical attributes of wheat genotypes. Cercet Agron Mold 46:49–67Google Scholar
- Lindsay WL, Norvell WA (1978) Development of a DTPA soil test for zinc, iron, manganese, and copper. Soil Sci Soc Amer J 42:421–428Google Scholar
- Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880Google Scholar
- Nkrumah PN, Chaney RL, Morel JL (2018) Agronomy of ‘Metal Crops’ Used in Agromining. In: Van der Ent, A, Echevarria G, Baker A, Morel JL (eds) Agromining: Farming for Metals. Extracting Unconventional Resources Using Plants. Springer, Berlin, pp 19–38 Google Scholar
- Singleton V, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 16:144–158Google Scholar