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Zinc accumulation and distribution over tissues in Noccaea сaerulescens in nature and in hydroponics: a comparison

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Abstract

Aims

Zinc distribution at the tissue level is studied almost exclusively in lab-grown plants. It is essential to establish to what extent the patterns observed in lab-grown plants are corresponding with those in nature. To this end, we compared Zn localization in Noccaea caerulescens growing in its natural environment, a zinc/lead mine tailing, with that in hydroponically grown plants of the same origin.

Methods

Zinc concentrations in plants and soil were determined by flame AAS and Zn localization in leaf tissues was studied using Zn indicators Zincon and Zinpyr-1.

Results

The mean Zn concentration in plants at the mine tailings was around 15,000 mg/kg DW, which corresponded well with the Zn concentration in the leaves of plants grown at 1600 μM Zn in the nutrient solution. The Zn distribution patterns in leaves of plants sampled from the mine and plants grown in hydroponics were identical. Zn-dependent staining was the most intensive in water-storage epidermal cells, guard cells and vascular bundles, and less intensive in subsidiary and mesophyll cells.

Conclusions

Zinc distribution in hydroponically grown plants is representative for plants in nature. Preferential Zn sequestration in leaves, particularly in water-storage epidermal cells, restricts metal accumulation in mesophyll and contributes to Zn hypertolerance.

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Acknowledgements

The authors are grateful to Victor Ivanov for critical discussion of the results, to Rudo Verweij, Rob Broekman, Richard van Logtestijn and Riet Vooijs for technical support, Marc Aarts and Patrick Doumas for providing the coordinates of the mines. This work was partially supported by the grants from the Russian Foundation for Basic Research (RFBR, № 15-04-02236) and from the international scientific program GDRI LOCOMET (Transport, localization and complexation of metals in hyperaccumulating plants) funded by The National Center for Scientific Research, as well as by the French Embassy Metchnikov scholarship funded by Campus France.

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Authors and Affiliations

  1. Laboratory of Root Physiology, Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya str. 35, 127276, Moscow, Russia

    Anna D. Kozhevnikova & I. V. Seregin

  2. Laboratoire de Biochimie et Physiologie Végétale, UMR5004 Bât. 7, 2 Place Pierre Viala, 34060, Montpellier Cedex 2, France

    F. Gosti

  3. Department of Ecological Sciences, Faculty of Earth and Life Sciences, Vrije Universiteit Amsterdam, de Boelelaan 1087, 1081 HV, Amsterdam, the Netherlands

    H. Schat

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  1. Anna D. Kozhevnikova
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  2. I. V. Seregin
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  3. F. Gosti
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  4. H. Schat
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Correspondence to Anna D. Kozhevnikova.

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Responsible Editor: Juan Barcelo.

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Kozhevnikova, A.D., Seregin, I.V., Gosti, F. et al. Zinc accumulation and distribution over tissues in Noccaea сaerulescens in nature and in hydroponics: a comparison. Plant Soil 411, 5–16 (2017). https://doi.org/10.1007/s11104-016-3116-6

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