Nutrient use preferences among soil Streptomyces suggest greater resource competition in monoculture than polyculture plant communities
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Background an aims
Nutrient use overlap among sympatric Streptomyces populations is correlated with pathogen inhibitory capacity, yet there is little information on either the factors that influence nutrient use overlap among coexisting populations or the diversity of nutrient use among soil Streptomyces.
We examined the effects of plant host and plant species richness on nutrient use of Streptomyces isolated from the rhizosphere of Andropogon gerardii (Ag) and Lespedeza capitata (Lc) growing in communities of 1 (monoculture) or 16 (polyculture) plant species. Growth on 95 carbon sources was assessed over 5d.
Cumulative growth was significantly greater for polyculture vs. monoculture isolates, and for Lc vs. Ag isolates. Isolates from monocultures, but not polycultures, exhibited a drop in growth rates between 24 h and 72 h post-inoculation, suggesting resource allocation to non-growth functions. Isolates from high-carbon (polyculture) or high-nitrogen (Lc) soils had larger niche widths than isolates from low-C (monocultures) or low-N (Ag) soils. Sympatric isolates from polycultures were significantly more differentiated from one another in preferred nutrients for growth than sympatric isolates from monocultures.
These results suggest that Streptomyces populations respond to selection imposed by plant host and plant community richness and that populations from polyculture but not from monoculture, mediate resource competition via niche differentiation.
KeywordsStreptomyces Andropogon gerardii Lespedeza capitata Plant richness
Adil Essarioui was supported by funds from the Islamic Development Bank. Research was supported by Agricultural and Food Research Grant Initiative Competitive Grant 2011-67019-30200 from the USDA National Institute of Food and Agriculture. Technical and field support from Lindsey Hanson, Dan Schlatter, and Nick LeBlanc were invaluable to completion of the work. Field plots maintained under National Science Foundation Long-Term Ecological Research Grant 0620652 were the source of soil samples used in this study.
- Becker DM, Kinkel LL (1999) Strategies for quantitative isolation of Streptomyces from soil for studies of pathogen ecology and disease biocontrol. Recent Research Developments in Microbiology 3(1):349–362Google Scholar
- Bremer C, Braker G, Matthies D, Beierkuhnlein C, Conrad R (2009) Plant presence and species combination, but not diversity, influence denitrifier activity and the composition of nirK -type denitrifier communities in grassland soil. FEMS Microbiol Ecol 70:377–387. doi: 10.1111/j.1574-6941.2009.00732.x CrossRefPubMedGoogle Scholar
- Czàràn T, Hoekstra R, Pagie L (2002) Chemical warfare between microbes promotes biodiversity. Proc Natl Acad Sci 99:786–790. doi: 10.1073/pnas.012399899
- Doumbou C, Hamby Salove M, Crawford D, Beaulieu C (2001) Actinomycetes, promising tools to control plant diseases and to promote plant growth. Phytoprotection 82:85. doi: 10.7202/706219ar
- Langenheder S, Székely AJ (2011) Species sorting and neutral processes are both important during the initial assembly of bacterial communities. The ISME Journal 5:1086–1094. doi: 10.1038/ismej.2010.207
- Nihorimbere V, Ongena M, Smargiassi M, Thonart P (2011) Beneficial effect of the rhizosphere microbial community fpr plant growth and health. Biotechnol Agron Soc Environ 15(2):327–337Google Scholar
- Omura S, Ikeda H, Ishikawa J, Hanamoto A, Takahashi C, Shinose M, Takahashi Y, Horikawa H, Nakazawa H, Osonoe T, Kikuchi H, Shiba T, Sakaki Y, Hattori M (2001) Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites. Proc Natl Acad Sci 98:12215–12220. doi: 10.1073/pnas.211433198 CrossRefPubMedPubMedCentralGoogle Scholar
- Schlatter D, Kinkel L (2015) Do tradeoffs structure antibiotic inhibition, resistance, and resource use among soil-borne Streptomyces? BMC Evol Biol. doi: 10.1186/s12862-015-0470-6
- Thonart P, Nihorimbere V, Ongena MM, Smargiassi M (2011) Beneficial effect of the rhizosphere microbial community for plant growth and health Biotechnologie. Agronomie Société et Environnement 15:327–337Google Scholar
- Wardle D, Bonner K, Barker G, Yeates G, Nicholson K, Bardgett R, Watson R, Ghani A (1999) Plant removals in perennial grassland: vegetation dynamics, decomposers, soil biodiversity, and ecosystem properties. Ecol Monogr 69:535–568. doi: 10.1890/0012-9615(1999)069[0535:pripgv]2.0.co;2 CrossRefGoogle Scholar