Pine wilt disease alters soil properties and root-associated fungal communities in Pinus tabulaeformis forest
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The objective was to elucidate how pine wilt disease impacts soil properties and root-associated fungi in Pinus tabulaeformis forest.
Soil responses to pine wilt disease were studied in three kinds of damaged (undisturbed, middle disturbed and high disturbed) stands. The responses of root-associated fungal community structure and diversity were examined through Illumina MiSeq sequencing of tagged amplicons of the 18S rDNA region. Soil nutrient properties were compared among stands using standard techniques.
Stands disturbed by pine wilt disease had lower soil hyphal density, pH, P and K content, higher organic matter and higher total N (P < 0.05) compared with undisturbed stands. With the increasing of disease damage, the root ergosterol content, the colonization rates of ectomycorrhizal fungi and total hyphae were significantly decreased (P < 0.05). Dark septate endopasitic fungi decreased firstly and then increased again with the increased severity of disease. The composition of root-associated fungi differed among three kinds of disturbed stands. Basidiomycota and Ascomycota were the dominant root-associated fungi of P. tabulaeformis. Tuber, as the most abundant fungal taxa, predominated in the undisturbed stand, while Russula in high disturbed stand. The community richness and diversity of root-associated fungi were significantly lower (P < 0.01) in high disturbed stand than that of undisturbed stand.
Pine wilt disease alters soil biotic and nutrient properties. Our study revealed clear differences in the biomass, abundance, diversity and community structure of root-associated fungi among three pure P. tabulaeformis stands. Analysis of dominated RAF abundance provides one of methods to predict the damage degree of pine wilt disease to some extent.
KeywordsPine wilt disease Root-associated fungi Fungal community Soil physichemical properties Pinus tabulaeformis
This study was supported by the National Natural Science Foundation of China (31300543, 31270639, 31170567), the Fundamental Research Funds for the Central Universities (Z109021308), Research Foundation for Advanced Talents (Z111021204) of Northwest A&F University and Shaanxi province, Program for Changjiang Scholars and Innovative Research Team in University of China (IRT1035). We acknowledge doctor Michael Allen for his insightful comments.
- Amato KR, Yeoman CJ, Kent A, Righini N, Carbonero F, Estrada A, Gaskins HR, Stumpf RM, Yildirim S, Torralba M (2013) Habitat degradation impacts black howler monkey (Alouatta pigra) gastrointestinal microbiomes. ISME J 7:1344–1353. doi: 10.1038/ismej.2013.16 CrossRefPubMedPubMedCentralGoogle Scholar
- Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, Fierer N, Pena AG, Goodrich JK, Gordon JI, Huttley GA, Kelley ST, Knights D, Koenig JE, Ley RE, Lozupone CA, McDonald D, Muegge BD, Pirrung M, Reeder J, Sevinsky JR, Tumbaugh PJ, Walters WA, Widmann J, Yatsunenko T, Zaneveld J, Knight R (2010) QIIME allows analysis of high-throughput community sequencing data. Nat Methods 7:335–336. doi: 10.1038/nmeth.f.303 CrossRefPubMedPubMedCentralGoogle Scholar
- Cheng H, Lin M, Li W (1983) Pine wilt disease found on the Pinus thunbergii in Nanjing. For Pest Dis 4:1–5 in ChineseGoogle Scholar
- Ge P, Da L, Wang W, Xu X (2014) Seasonal dynamics of dissolved organic carbon, nitrogen and other nutrients in soil of Pinus massoniana stands after pine wilt disease disturbance. J Soil Sci Plant Nut 14:75–87Google Scholar
- Guan SY, Zhang D, Zhang Z (1986) Soil enzyme and its research methods. Agricultural, BeijingGoogle Scholar
- Kikuchi J, Tsuno N, Futai K (1991) The effect of mycorrhizae as a resistance factor of pine trees (Pinus densiflora) to the pinewood nematode. J Jpn for Soc 73:216–218Google Scholar
- Kiyohara T, Tokushige Y (1971) Inoculation experiments of a nematode, Bursaphelenchus sp., onto pine trees. J Jpn for Soc 53:210–218Google Scholar
- Proença DN, Francisco R, Santos CV, Lopes A, Fonseca L, Abrantes IM, Morais PV (2010) Diversity of bacteria associated with Bursaphelenchus xylophilus and other nematodes isolated from Pinus pinaster trees with pine wilt disease. PLoS One 5(12):e15191. doi: 10.1371/journal.pone.0015191 CrossRefPubMedPubMedCentralGoogle Scholar
- Smith SE, Read DJ (2010) Mycorrhizal symbiosis. Academic Press, New YorkGoogle Scholar
- Southey JF (1986) Laboratory methods for work with plant and soil nematodes. HMSO Press, LondonGoogle Scholar
- Steiner G, Buhrer EM (1934) Aphelenchoides xylophilus n. sp., a nematode associated with blue-stain and other fungi in timber. J Agric Res 48:949–951Google Scholar
- Tan KH (2005) Soil sampling, preparation, and analysis. CRC Press, Boca RatonGoogle Scholar
- Xu Z, Ward S, Chen C, Blumfield T, Prasolova N, Liu J (2008) Soil carbon and nutrient pools, microbial properties and gross nitrogen transformations in adjacent natural forest and hoop pine plantations of subtropical Australia. J Soils Sediments 8:99–105. doi: 10.1065/jss2008.02.276 CrossRefGoogle Scholar
- Yano M (1913) Investigation of the causes of pine mortality in Nagasaki Prefecture. Sanrin Koho 4 (Suppl.): l-14 in JapaneseGoogle Scholar
- Yi CK, Byun BH, Park JD, Yang S, Chang KH (1989) First finding of the pine wood nematode, Bursaphelenchus xylophilus (Steiner & Buhrer) Nickle and its insect vector in Korea. Research Reports of the Forestry Research Institute (Seoul):141–149Google Scholar
- Zhang HH, Tang M, Chen H, Wang YJ, Ban YH (2010) Arbuscular mycorrhizas and dark septate endophytes colonization status in medicinal plant Lycium barbarum l. in arid Northwestern China. Afr J Microbiol Res 4:1914–1920Google Scholar