Large-scale profiling of brown rice ionome in an ethyl methanesulphonate-mutagenized hitomebore population and identification of high- and low-cadmium lines
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Improving the nutritional value of cereal grains and reducing the intake of toxic elements, such as cadmium (Cd), from grains contribute to food quality.
Here, we report a large-scale profiling of the brown rice ionome in an ethyl methanesulphonate (EMS)-mutagenized population of the commercial rice cultivar ‘Hitomebore’. Broad-sense heritability for manganese, zinc, and cesium were greater than 0.5, suggesting that the EMS population is suitable for screening mutants with different concentrations of these elements.
From the EMS-mutagenized population, we isolated two mutant lines with high and low Cd levels. Sequence analysis revealed that the high-Cd line had a mutation at position 105 (leucine to histidine) in OsHMA3, a gene that regulates Cd accumulation in rice shoots. The low-Cd line had a mutation at position 242 (histidine to glutamine) in OsNramp5, a gene responsible for Cd uptake. Agronomical traits of the low-Cd line were indistinguishable from those of the wild type, indicating that the low-Cd line which we isolated is applicable to Hitomebore farming areas.
The present study shows the effectiveness of mutant lines derived from elite cultivars in developing agriculturally useful lines with different mineral contents.
KeywordsHitomebore Ionome Mineral concentration in rice grain OsNramp5
inductively coupled plasma mass spectrometry
broad sense heritability
coefficient of variance
We greatly appreciate Dr Ryohei Terauchi for providing us with the mutagenized population of Hitomebore. Dr. Satoru Ishikawa for his constructive discussion on the present study. This study was supported in part by a grant from Steel Foundation for Environmental Protection Technology (to S. N.), and by grants from the JSPS (Grant-in-Aid for Scientific Research numbers 25221202 and 25114506) to T.F.
- Falconer DS, Mackay TFC (1996) An introduction to quantitative genetics, 4th edn. Prentice Hall, LondonGoogle Scholar
- Food and Agriculture Organization/World Health Organization 2010 Joint FAO/ WHO Expert Committee on Food Additives, Seventy-Third Meeting, Geneva, 8–17, June 2010Google Scholar
- Institute of Medicine/Food and Nutrition Board (2001) Dietary reference intakes: vitamin A, vitamin K, arsenic, boron, chromium, copper, iodine, iron, manganese, molybdenum, nickel, silicon, vanadium and zinc. National Academy Press, WashingtonGoogle Scholar
- Ishikawa S, Ishimaru Y, Igura M, Kuramata M, Abe T, Senoura T, Hase Y, Arao T, Nishizawa NK, Nakanishi H (2012) Ion-beam irradiation, gene identification, and marker-assisted breeding in the development of low-cadmium rice. Proc Natl Acad Sci U S A 109:19166–19171CrossRefPubMedPubMedCentralGoogle Scholar
- Masuda H, Ishimaru Y, Aung MS, Kobayashi T, Kakei Y, Takahashi M, Higuchi K, Nakanishi H, Nishizawa NK (2012) Iron biofortification in rice by the introduction of multiple genes involved in iron nutrition. Sci Rep 2:534Google Scholar
- Miyadate H, Adachi S, Hiraizumi A, Tezuka K, Nakazawa N, Kawamoto T, Katou K, Kodama I, Sakurai K, Takahashi H, Satoh-Nagasawa N, Watanabe A, Fujimura T, Akagi H (2011) OsHMA3, a P1B-type of ATPase affects root-to-shoot cadmium translocation in rice by mediating efflux into vacuoles. New Phytol 189:190–199CrossRefPubMedGoogle Scholar
- Stoltzfus RJ, Dreyfuss ML (1998) Guidelines for the use of iron supplements to prevent and treat iron deficiency anemia. ILSI Press, WashingtonGoogle Scholar
- Theil EC (2003) Ferritin: at the crossroads of iron and oxygen metabolism. J Nutr 133:1549–1553Google Scholar
- World Health Organization (WHO) (2002) The world health report. Reducing risks, promoting healthy life. World Health Organization (WHO), GenevaGoogle Scholar