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Plant and Soil

, Volume 401, Issue 1–2, pp 213–229 | Cite as

Volatiles from biofumigant plants have a direct effect on carpogenic germination of sclerotia and mycelial growth of Sclerotinia sclerotiorum

  • Rachel Warmington
  • John P. Clarkson
Regular Article

Abstract

Aims

Sclerotia of Sclerotinia sclerotiorum survive in soil and germinate to produce apothecia which release airborne ascospores. Current control methods rely predominantly on the use of fungicides to kill ascospores. The aim of this research was to identify potential biofumigation treatments which suppress sclerotial germination, providing a potential alternative and long-term approach to disease management.

Methods

Microcosm and in vitro experiments were conducted using dried and milled plant material from six different biofumigant crop plants to determine effects on carpogenic germination of sclerotia and mycelial growth of S. sclerotiorum.

Results

All biofumigant plants significantly reduced germination of S. sclerotiorum sclerotia in the microcosm experiments, but were less effective against larger sclerotia. In vitro experiments showed a direct effect of biofumigant volatiles on both the mycelial growth of S. sclerotiorum, and carpogenic germination of sclerotia, where the most effective treatment was B. juncea ‘Vittasso’.

Conclusions

It was clear from this study that biofumigant crop plants have potential as part of an integrated disease management system for control of S. sclerotiorum. The microcosm experiments described here provide a straightforward and reliable screening method for evaluating different biofumigants for activity.

Keywords

Sclerotinia sclerotiorum Biofumigation Glucosinolate Isothiocyanate Brassica 

Notes

Acknowledgments

This work was carried out as part of a PhD studentship (CP80) funded by the Horticultural Development Company, UK. The author acknowledges assistance from Andrew Jukes and Julie Jones at Warwick Crop Centre for their assistance and guidance with HPLC analysis and statistical analysis, respectively.

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  1. 1.Eden Project, BodelvaCornwallUK
  2. 2.Warwick Crop Centre, School of Life SciencesUniversity of WarwickWellesbourneUK

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