Genotypic differences in antioxidant response to phosphorus deficiency in Brassica napus
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Background and aims
The effect of phosphorus (P) deficiency on plant growth and development depends on the ability of plants to adapt to induced stress. Oxidative stress may play a vital role in P deficient Brassica napus and the antioxidant ability of this plant may be an important mechanism for adapting to P deficiency. The present study aims to better understand the correlation between high P use efficiency and antioxidative ability by comparing the activity of antioxidant enzymes and lipid peroxidation in the leaves and roots of two B. napus genotypes.
The response of the antioxidant defense system of two B. napus genotypes with different P use efficiencies to short-term P-free starvation and long-term low P stress were investigated by analyzing the activities of antioxidative enzymes, the superoxide free radical ion (O2 ·−) and H2O2 concentration, and the level of lipid peroxidation.
The activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (GPX), and glutathione reductase (GR) in the leaves and roots were increased in both genotypes under the two P treatments. However, the activities of some of these enzymes exhibited tissue or genotype specificity. In the P use-inefficient genotype “B104-2,” more O2 ·− was accumulated and a higher level of lipid peroxidation was induced under P deficiency conditions compared with P use-efficient genotype “Eyou Changjia.” The GPX activity in the roots was considerably higher (20- to 30-fold) than in the leaves, whereas the CAT activity in the roots was lower (approximately 3-fold) than in the leaves in both genotypes.
Compared with P use-inefficient genotype “B104-2,” P use-efficient genotype “Eyou Changjia” suffered less oxidative damage and lipid peroxidation, and inhibited O2 ·− generation more effectively under low P stress. Severe oxidative damage was accompanied with a higher antioxidative enzyme activities in “B104-2” under low P stress.
KeywordsP deficiency Oxidative stress Antioxidative enzymes Lipid peroxidation Brassica napus Genotypic difference
This work was supported by the grants from the National Natural Science Foundation (31172019) and National Basic Research and Development Program (2011CB100301), China.
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