Naturalised populations of mesorhizobia in chickpea (Cicer arietinum L.) cropping soils: effects on nodule occupancy and productivity of commercial chickpea
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Background and aims
Chickpea rhizobia did not occur naturally in Australian cropping soils, necessitating inoculation at sowing. Now, after more than 30 years of chickpea cultivation using a single inoculant strain, CC1192, it is likely that chickpea rhizobia are established in 1.0–1.5 Mha cropping land. The aims of this study were to examine effects of the naturalised chickpea rhizobia on nodulation and productivity (total crop N, crop N fixed and grain yield) of commercial chickpea.
Soil was sampled from 26 fields to estimate chickpea rhizobial numbers, relate numbers to edaphic factors and years since previous chickpea crop, determine the proportions of CC1192 and novel strains using RAPD-PCR and subject a subset of novel strains from one site to 16S rRNA analysis. Nodules were harvested from 15 inoculated, commercial chickpea crops to determine occupancy by CC1192. The symbiotic effectiveness of a second subset of novel strains was assessed.
The mean number of rhizobia in the soils varied from log 0.08 to log 5.16 rhizobia g soil−1 with population size positively correlated with soil moisture content and negatively correlated with salt concentration (ECe). RAPD-PCR analysis of 570 strains of chickpea rhizobia isolated from the soils indicated only 14 % with molecular fingerprints similar to CC1192. Occupancy by CC1192 of nodules harvested from commercial crops ranged 0–100 %, with an average of 53 %. Occupancy by CC1192 declined by an average 17 % with each log unit increase in numbers of novel chickpea rhizobia.
We found no evidence that the novel mesorhizobia in the chickpea soils compromised N2 fixation or productivity of commercial chickpea crops, even though individual strains had generally reduced symbiotic effectiveness relative to CC1192.
KeywordsEdaphic N2 fixation RAPD-PCR Genetic divergence Rhizobial population
We thank Dr Alison McInnes, formerly of the University of Western Sydney, for scientific and technical direction and Judith Gray, University of Western Sydney, for assistance with the RAPD-PCR analysis. We gratefully acknowledge Dr Kemanthie Nandasena, Centre for Rhizobium Studies (CRS), Murdoch University, Western Australia, for the 16S rRNA gene sequencing and for her considerable and unselfish assistance in interpreting the results We also thank Professor Graham O’Hara also from the CRS, Murdoch University, for providing additional interpretation of and comment on the sequencing data. We acknowledge the Australian Grains Research & Development Corporation (GRDC) for financial support for the post-graduate scholarship (NE) and project support.
- Barcellos FG, Menna P, Batista JSD, Hungria M (2007) Evidence of horizontal transfer of symbiotic genes from a Bradyrhizobium japonicum inoculant strain to indigenous diazotrophs Sinorhizobium (Ensifer) fredii and Bradyrhizobium elkanii in a Brazilian Savannah soil. Appl Environ Microb 732635–2643Google Scholar
- Daniels I, Manning B, Pearce L (2002) Profile descriptions: district guidelines for managing soils in north-west NSW. NSW Agriculture, SydneyGoogle Scholar
- Elias NV, Herridge DF (2014) Crop-available water and agronomic management, rather than nitrogen supply, primarily determine grain yield of commercial chickpea in northern New South Wales. Crop Pasture Sci 65:442–452 http://dx.doi.org/ 10.1017/CP13397
- FAOSTAT (2013) FAO Statistics Division, viewed on 15 November 2013, http://faostat.fao.org/site/567/DesktopDefault.aspx?PageID = 567#ancor
- Herridge D (1977) Carbon and nitrogen nutrition of two annual legumes. University of Western Australia, PhDGoogle Scholar
- Herridge D (2008) Inoculation technology for legumes. In: Dilworth MJ, James EK, Sprent JI, Newton WE (eds) Nitrogen-fixing leguminous-symbioses. Springer, Dordrecht, The Netherlands, pp 77–109Google Scholar
- Marshall TJ (1947) Mechanical composition of soil in relation to field descriptions of texture. Bulletin No. 224. Council for Scientific and Industrial Research Bulletin, Melbourne, AustraliaGoogle Scholar
- Nandasena KG, O'Hara GW, Tiwari RP, Sezmis E, Howieson JG (2007) In situ lateral transfer of symbiosis islands results in rapid evolution of diverse competitive strains of mesorhizobia suboptimal in symbiotic nitrogen fixation on the pasture legume Biserrula pelecinus L. Environ Microbiol 9:2496–2511PubMedCrossRefGoogle Scholar
- Rayment GE, Higginson FR (1992) The Australian laboratory handbook of soil and water chemical methods. Inkata Press, SydneyGoogle Scholar
- Rivas R, Laranjo M, Mateos PF, Oliveira S, Martinez-Molina E, Velazquez E (2007) Strains of Mesorhizobium amorphae and Mesorhizobium tianshanense, carrying symbiotic genes of common chickpea endosymbiotic species, constitute a novel biovar (ciceri) capable of nodulating Cicer arietinum. Lett Appl Microbiol 44:412–418PubMedCrossRefGoogle Scholar
- Rupela OP, Saxena MC (1987) Nodulation and nitrogen fixation in chickpea. The chickpea. CAB International, Wallingford, UK, In, pp 191–206Google Scholar
- Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: A laboratory manual 2nd Ed. Cold Spring Harbour Laboratory Press. Cold Spring Harbour, New YorkGoogle Scholar
- Soil Survey Staff (2010) Keys to soil taxonomy (11th Edition). USDANRCS, US Gov. Print. Office, Washington, DC, USAGoogle Scholar
- Thies JE, Bohlool BB, Singleton PW Environmental effects on competition for nodule occupancy between introduced and indigenous rhizobia and among introduced strains. Can J Microbiol 38:493–500 (1992)Google Scholar
- Wadisirisuk P, Danso SKA, Hardarson G, Bowen GD (1989) Influence of Bradyrhizobium japonicum location and movement on nodulation and nitrogen fixation in soybeans. Appl Environ Microb 55:1711–1716Google Scholar