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Overexpression of citrate operon in Herbaspirillum seropedicae Z67 enhances organic acid secretion, mineral phosphate solubilization and growth promotion of Oryza sativa

Abstract

Background and aims

Herbaspirillum seropedicae Z67, nitrogen fixing endophyte, significantly promotes the growth of cereals. Organic acid secreting nitrogen fixing rhizobacteria have better plant growth promotion abilities due to mineral phosphate solubilization.

Method

Plasmids pAB7, pJNK3 and pJNK4 containing Escherichia coli cs (gltA), NADH insensitive cs (gltA1), and citrate operon consisting of gltA1 gene along with Salmonella typhimurium Na+ dependent citrate transporter (citC) gene under constitutive lac promoter were constructed in broad host range plasmid pUCPM18-Kmr. The plasmid transformants of H. seropedicae Z67 were obtained by electroporation.

Results

Hs (pAB7) and Hs (pJNK3) had increased CS activity but citric acid secretion was not significant. Hs (pJNK3) secreted 45 mM acetic acid while Hs (pJNK4) secreted 2.7 mM citric and 51 mM acetic acids. Hs (pJNK3) and Hs (pJNK4) released 80 μM and 110 μM amount of P from rock phosphate, respectively, in buffered medium under both aerobic and micro aerobic conditions. These transformants showed better plant growth promoting factors. Upon inoculation to rice plants (Gujarat – 17), increase of Fresh weight, Dry weight N, P and K content was observed.

Conclusion

Thus the study demonstrates that artificial citrate operon in H. seropedicae Z67 enhances phosphate solubilization and plant growth promotion abilities.

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Correspondence to G. Naresh Kumar.

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Wagh, J., Bhandari, P., Shah, S. et al. Overexpression of citrate operon in Herbaspirillum seropedicae Z67 enhances organic acid secretion, mineral phosphate solubilization and growth promotion of Oryza sativa . Plant Soil 383, 73–86 (2014). https://doi.org/10.1007/s11104-014-2161-2

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  • DOI: https://doi.org/10.1007/s11104-014-2161-2

Keywords

  • Citrate synthase
  • Citrate secretion
  • Citrate transporter
  • Mineral phosphate solubilization
  • Rice