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Overexpression of citrate operon in Herbaspirillum seropedicae Z67 enhances organic acid secretion, mineral phosphate solubilization and growth promotion of Oryza sativa

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Abstract

Background and aims

Herbaspirillum seropedicae Z67, nitrogen fixing endophyte, significantly promotes the growth of cereals. Organic acid secreting nitrogen fixing rhizobacteria have better plant growth promotion abilities due to mineral phosphate solubilization.

Method

Plasmids pAB7, pJNK3 and pJNK4 containing Escherichia coli cs (gltA), NADH insensitive cs (gltA1), and citrate operon consisting of gltA1 gene along with Salmonella typhimurium Na+ dependent citrate transporter (citC) gene under constitutive lac promoter were constructed in broad host range plasmid pUCPM18-Kmr. The plasmid transformants of H. seropedicae Z67 were obtained by electroporation.

Results

Hs (pAB7) and Hs (pJNK3) had increased CS activity but citric acid secretion was not significant. Hs (pJNK3) secreted 45 mM acetic acid while Hs (pJNK4) secreted 2.7 mM citric and 51 mM acetic acids. Hs (pJNK3) and Hs (pJNK4) released 80 μM and 110 μM amount of P from rock phosphate, respectively, in buffered medium under both aerobic and micro aerobic conditions. These transformants showed better plant growth promoting factors. Upon inoculation to rice plants (Gujarat – 17), increase of Fresh weight, Dry weight N, P and K content was observed.

Conclusion

Thus the study demonstrates that artificial citrate operon in H. seropedicae Z67 enhances phosphate solubilization and plant growth promotion abilities.

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Authors and Affiliations

  1. Molecular Microbial Biochemistry Laboratory, Department of Biochemistry, Faculty of Science, M. S. University of Baroda, Vadodara, 390 002, India

    Jitendra Wagh, Praveena Bhandari, Sonal Shah & G. Naresh Kumar

  2. Department of Microbiology and Biotechnology Center, Faculty of Science, M. S. University of Baroda, Vadodara, 390 002, India

    G. Archana

  3. Department of Biochemistry, Faculty of Science, M. S. University of Baroda, Sayajigunj, Vadodara, 390 002, Gujarat, India

    G. Naresh Kumar

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  1. Jitendra Wagh
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  2. Praveena Bhandari
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  3. Sonal Shah
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  4. G. Archana
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Correspondence to G. Naresh Kumar.

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Wagh, J., Bhandari, P., Shah, S. et al. Overexpression of citrate operon in Herbaspirillum seropedicae Z67 enhances organic acid secretion, mineral phosphate solubilization and growth promotion of Oryza sativa . Plant Soil 383, 73–86 (2014). https://doi.org/10.1007/s11104-014-2161-2

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