Physiological and biochemical impacts of magnesium-deficiency in two cultivars of coffee
- 661 Downloads
To evaluate biochemical and physiological impacts of magnesium-deficiency on seedlings of two cultivars (Catuaí and Acaiá) of Coffea arabica L..
Six month old seedlings from both cultivars were transferred to plastic receptacles containing solutions with different concentrations of magnesium (Mg). Fully expanded leaves and roots were evaluated at the beginning of treatment and after 10, 20 and 30 days for chlorophyll and carotenoid content, biomass allocation, partitioning of carbohydrates and antioxidant metabolism.
Mg-deficiency was characterized by an increase in the shoot/root dry weight ratio, which may be related to accumulation of carbohydrates in leaves. This accumulation is probably responsible for triggering a reduction in the consumption of reducing equivalents, providing favorable conditions for the formation of reactive oxygen species (ROS). The increase in ROS production was accompanied by increases in ascorbate concentration and enzyme activity of the antioxidant metabolism.
The Catuaí cultivar is more sensitive to Mg-deficiency than the Acaiá cultivar. When exposed to magnesium deficiency the Catuaí cultivar had reduced growth and its antioxidant metabolism was less efficient at removing ROS.
KeywordsCarbohydrate partitioning Biomass allocation Chlorophyll Oxidative stress
The authors would like to thank Dr. Antônio Eduardo Furtini Neto. The reserach was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
- Balakrishnan K, Rajendran C, Kulandaivelu G (2001) Differential responses of iron, magnesium, and zinc deficiency on pigment composition, nutrient content, and photosynthetic activity in tropical fruit crops. Photosynthetica 38:477–479. doi: 10.1023/A:1010958512210
- Bouché-Pillon S, Fleurat-Lessard P, Fromont JC, Serrano R, Bonnemain JL (1994) Immunolocalization of the plasma membrane H+-ATPase in minor veins of Vicia faba in relation to phloem loading. Plant Physiol 105:691–697. doi: 10.1104/pp.105.2.691
- Blair JM (1970) Magnesium, potassium and the adenylate kinase equilibrium. Magnesium as feedback signal from the adenine nucleotide pool. Eur J Biochem 13:384–390. doi: 10.1111/j.1432-1033.1970.tb00940.x
- Cakmak I, Yazici AM (2010) Magnesium: a forgotten element in croop production. Better Crops 94:23–25Google Scholar
- Carvalho CHS (2008) Cultivares de café: origem, características e recomendações. Embrapa Café, BrasíliaGoogle Scholar
- Ceppi G (2010) Parametres photosynthetiques affectant le transport d’electrons a travers le pool de plastoquinone: la densite des photosystemes I, le contenu de chlorophylle et l’activite d’une plastoquinol-oxydase PhD-thesis. University of Geneva, GenevaGoogle Scholar
- Ceppi MG, Oukarroum A, Nuran C, Strasser RJ, Schansker G (2012) The IP amplitude of the fluorescence rise OJIP is sensitive to changes in the photosystem I content of leaves: a study on plants exposed to magnesium and sulfate deficiencies, drought stress and salt stress. Physiol Plant 144:277–288. doi: 10.1111/j.1399-3054.2011.01549.x PubMedCrossRefGoogle Scholar
- Dische Z (1962) General color reactions. In: Whistler RL, Wolfram ML (eds) Carbohydrate chemistry. Academic, New York, pp 477–520Google Scholar
- Fernandes KD, Paiva PDO, Carvalho JG, Resende AC, Figueiredo MA (2012) Multiple nitrogen and phosphorus deficiency in Zantedeschia. Cienc Agrotec 36:631–638. doi: 0.1590/S1413-70542012000600005Google Scholar
- Gechev TS, Van Breusegem F, Stone JM, Denev I, Laloi C (2006) Reactive oxygen species as signals that modulate plant stress responses and programmed cell death. Bioessays 28:1091–1101. doi:/ 10.1104/pp.106.078295
- Getz HP, Kleins M (1995) The vacuolar ATPase of red beet storage tissue: electron microscopic demonstration of the “head-andstalk” structure. J Plant Physiol 108:14–23Google Scholar
- Giannopolitis CN, Ries SK (1977) Superoxide dismutases: I. occurrence in higher plants. Plant Physiol 59:309–314. doi: 10.1104/pp.59.2.309
- Guimarães RJ, Mendes ANG, Souza CAS (2002) Cafeicultura. FAEPE/UFLA, LavrasGoogle Scholar
- Havir EA, Mchale NA (1987) Biochemical and developmental characterization of multiple forms of catalase in tobacco leaves. Plant Physiol 84 :450–455. doi: 10.1104/pp.84.2.450
- Hermans C, Bourgis F, Faucher M, Strasser RJ, Delrot S, Verbruggen N (2004) Physiological characterization of magnesium deficiency in sugar beet: acclimation to low magnesium differentially affects photosystems I and II. Planta 220:344–355. doi: 10.1007/s00425-004-1340-4 PubMedCrossRefGoogle Scholar
- Hoagland D, Arnon DI (1950) The water culture method for growing plants without soil. Calif Agr Exp Stn 42:1–32Google Scholar
- Jimenez A, Hernández JA, Pastori G, del Río LA (1998) Role of the ascorbate-glutathione cycle of mitochondria and peroxisomes in the senescence of pea leaves. Plant Physiol 118:1327–1335. doi: 10.1104/pp.118.4.1327
- Kura-Hotta M, Satoh K, Katoh S (1987) Relationship between Photosynthesis and Chlorophyll Content during Leaf Senescence of Rice Seedlings. Plant Cell Physiol 28:1321–1329Google Scholar
- Lavon R, Salomon R, Goldschmidt EE (1999) Effect of potassium, magnesium, and calcium deficiencies on nitrogen constituents and chloroplast components in Citrus leaves. J Am Soc Hort Sci 124:158–162Google Scholar
- Lichtenthaler HK (2001) Buschmann C (2001) Chlorophylls and carotenoids: measurement and characterization by UV–VIS spectroscopy. In: Wrolstad RE, Acree TE, Decker EA, Penner MH, Reid DS, Schwartz ST, Shoemaker CF, Smith DS, Sporns P (eds) Current protocols in food analytical chemistry. Wiley, Davis, pp F4.3.1–F4.3.8Google Scholar
- Lu YK, Chen YR, Yang CM (1995) Influence of Fe- and Mg- deficiency on the thylakoid membranes of a chlorophylldeficient ch5 mutant of Arabidopsis thaliana. Bot Bull Acad Sin 36:175–179Google Scholar
- Malavolta E (1993) Nutrição mineral e adubação do cafeeiro: colheitas econômicas máximas, 1st edn. Agronômica Ceres, São PauloGoogle Scholar
- Malavolta E, Vitti GC, Oliveira AS (1989) Avaliação do estado nutricional das plantas, 1st edn. FEALQ, PiracicabaGoogle Scholar
- Martinez HEP, Menezes JFS, de Souza RB, Venegas VHA, Guimarães PTG (2004) Nutrição mineral, fertilidade do solo e produtividade do cafeeiro nas regiões de Patrocínio, Manhuaçu, Viçosa, São Sebastião do Paraíso e Guaxupé. EPAMIG, Belo HorizonteGoogle Scholar
- Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbato-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880Google Scholar