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Isolated cell walls exhibit cation binding properties distinct from those of plant roots

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Abstract

Aims

The principal contributor to the cation binding properties of roots is currently considered to be the cell wall or, alternatively, the plasma membrane. The aim of this study was to highlight their respective contributions in the binding properties.

Methods

Cell walls of a dicotyledon (Solanum lycopersicum L.) and monocotyledon (Triticum aestivum L.) were isolated from roots and their binding properties were compared to those of their respective roots. Cell wall and root binding capacities were evaluated by potentiometric titrations and cation exchange capacity measurements, while their biochemical composition was analyzed by 13C-NMR spectroscopy.

Results

The lower binding capacity of isolated cell walls compared to roots revealed that cell plasma membranes had a higher binding site density than cell walls. The significant decrease in some NMR signals, i.e. carbonyl C, N alkyl/methoxyl C and alkyl C regions, suggested that carboxyl, amine and phosphate binding sites, borne by proteins and phospholipid plasma membranes, contribute to the binding capacity.

Conclusions

Cell walls and plasma membranes were found to be jointly involved in root binding properties and their respective contributions seemed vary between plants.

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Acknowledgments

The authors are grateful to French Environment and Energy Management Agency (ADEME) and the French Centre of Agricultural Research for Development (CIRAD) for funding the PhD scholarship of Stéphanie Guigues and INSU (CNRS) for funding the study via the EC2CO-CYTRIX call. The authors thank Patrick Cazevieille and Claire Chevassus-Rosset (CIRAD) for their technical support during the plant growth phase, Hélène Miche (CEREGE) for providing access to ICP-AES and Jean-Claude Davidian (Montpellier SupAgro) for his advice on root cell wall isolation.

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Authors and Affiliations

  1. CIRAD, UPR Recyclage et risque, F-34398, Montpellier, France

    Stéphanie Guigues & Emmanuel Doelsch

  2. ADEME, 20 avenue du Grésillé, BP-90406, Angers cedex 01, France

    Stéphanie Guigues

  3. CIRAD, UPR Recyclage et risque, F-97408, Saint-Denis, Réunion, France

    Matthieu N. Bravin

  4. Université de Toulon, PROTEE, EA 3819, 83957, La Garde, France

    Cédric Garnier

  5. Aix-Marseille Université, CNRS, IRD, CEREGE UM34, 13545, Aix-en-Provence, France

    Armand Masion

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  1. Stéphanie Guigues
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  2. Matthieu N. Bravin
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  3. Cédric Garnier
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  4. Armand Masion
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  5. Emmanuel Doelsch
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Correspondence to Stéphanie Guigues.

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Guigues, S., Bravin, M.N., Garnier, C. et al. Isolated cell walls exhibit cation binding properties distinct from those of plant roots. Plant Soil 381, 367–379 (2014). https://doi.org/10.1007/s11104-014-2138-1

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