N2 fixation of pea hypernodulating mutants is more tolerant to root pruning than that of wild type
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Background and aims
As a legume, pea plant has the ability to symbiotically fix N2. However, symbiotic N2 fixation is very sensitive to environmental stresses that affect plant growth, and there is little knowledge on the impact of root pruning on N2 fixation and plant growth.
In this study, we removed half of the nodulated roots of pea wild-type Frisson and hypernodulating mutants P64, P118, and P121. Dinitrogen fixation was measured using 15N labeling and carbon assimilation and partitioning between plant organs using 13C labeling.
Root pruning decreased N2 fixation by −46 to −79 % in wild-type and mutants. Pea mutant P118 had a lower decrease of specific activity of N2 fixation (−17 %) than both wild-type and other mutants (−36 to −62 %). For all genotypes, root pruning increased root and nodule sinks strengths for carbon. For P118 and for P121, this was associated to higher nodule growth than for control plants, as measured 8 days after root pruning.
This is the first analysis of N2-fixing plant response to root pruning. Importantly, we showed that some hypernodulating mutant pea lines (P118 and to a lesser extent P121) withstood this stress better than wild-type did.
KeywordsPisum sativum L Hypernodulating mutants Root pruning Symbiotic N2 fixation C nutrition Growth
Specific leaf nitrogen
Days after imbibition
Autoregulation of nodulation
Our grateful thanks are due to Vincent Durey, Patrick Mathey, Anne-Lise Santoni, and Sylvie Girodet for their technical assistance. We also thank Anouk Zancarini, Annabelle Larmure, Marion Prudent, Stephen Marle, and Charlotte Ollagnier for either their advice or help during the experiments. We thank the greenhouse staff for managing the experiments. We finally thank Richard Thompson and Sergio Ochatt for critical readings of this manuscript. This work was partly funded by the French National Institute of Agronomical Research (INRA), UNIP, and Burgundy Region.
- Chaudhary MI, Adu-Gyamfi JJ, Saneoka H, Nguyen NT, Suwa R, Kanai S, El-Shemy HA, Lightfoot DA, Fujita K (2008) The effect of phosphorus deficiency on nutrient uptake, nitrogen fixation and photosynthetic rate in mashbean, mungbean and soybean. Acta Physiol Plant 30(4):537–544. doi: 10.1007/s11738-008-0152-8 CrossRefGoogle Scholar
- Crozat Y, Dore T (2010) Biotic stresses. In: Munier-Jolain N, Biarnes V, Chaillet I, Lecoeur J, Jeuffroy M-H (eds) Physiology of the pea crop. QUAE edn. CRC Press, pp 193–196Google Scholar
- Crozat Y, Fustec J (2006) Assessing the role of grain legumes in crop rotation: some agronomic concepts that can help! In: AEP (ed) Grain legumes and the environment: how to assess benefits and impacts. Proceedings of the AEP workshop. Zürich, Switzerland, pp 55–60Google Scholar
- Evans JR, Seemann JR (1989) The allocation of protein-nitrogen in the photosynthetic apparatus : costs, consequences and control. In: Briggs W (ed) Toward a broad understanding of photosynthesis. ARLiss, New York, pp 183–205Google Scholar
- Frings JFJ (1976) The Rhizobium-Pea symbiosis as affected by high temperatures. Medelingen Landbouwhoges School, Wageningen, NetherlandGoogle Scholar
- Gerard PJ (2002) Nodule damage by clover root weevil larvae in white clover swards. In: New Zealand Plant Protection, Vol 55, vol 55. New Zealand Plant Protection-Series. New Zealand Plant Protection Soc, Rotorua, pp 246–251Google Scholar
- Hamon C, Baranger A, Coyne CJ, McGee RJ, Le Goff I, L'Anthoene V, Esnault R, Riviere JP, Klein A, Mangin P, McPhee KE, Roux-Duparque M, Porter L, Miteul H, Lesne A, Morin G, Onfroy C, Moussart A, Tivoli B, Delourme R, Pilet-Nayel ML (2011) New consistent QTL in pea associated with partial resistance to Aphanomyces euteiches in multiple French and American environments. Theor Appl Genet 123(2):261–281. doi: 10.1007/s00122-011-1582-z PubMedCrossRefGoogle Scholar
- Krusell L, Madsen LH, Sato S, Aubert G, Genua A, Szczyglowski K, Duc G, Kaneko T, Tabata S, de Bruijn F, Pajuelo E, Sandal N, Stougaard J (2002) Shoot control of root development and nodulation is mediated by a receptor-like kinase. Nature 420(6914):422–426. doi: 10.1038/nature01207 PubMedCrossRefGoogle Scholar
- Krusell L, Sato N, Fukuhara I, Koch BEV, Grossmann C, Okamoto S, Oka-Kira E, Otsubo Y, Aubert G, Nakagawa T, Sato S, Tabata S, Duc G, Parniske M, Wang TL, Kawaguchi M, Stougaard J (2011) The Clavata2 genes of pea and Lotus japonicus affect autoregulation of nodulation. Plant J 65(6):861–871. doi: 10.1111/j.1365-313X.2010.04474.x PubMedCrossRefGoogle Scholar
- Mahieu S, Germon F, Aveline A, Hauggaard-Nielsen H, Ambus P, Jensen ES (2009) The influence of water stress on biomass and N accumulation, N partitioning between above and below ground parts and on N rhizodeposition during reproductive growth of pea (Pisum sativum L.). Soil Biol Biochem 41(2):380–387. doi: 10.1016/j.soilbio.2008.11.021 CrossRefGoogle Scholar
- Moussart A, Onfroy C, Lesne A, Esquibet M, Grenier E, Tivoli B (2007) Host status and reaction of Medicago truncatula accessions to infection by three major pathogens of pea (Pisum sativum) and alfalfa (Medicago sativa). Eur J Plant Pathol 117(1):57–69. doi: 10.1007/s10658-006-9071-y CrossRefGoogle Scholar
- Munier-Jolain N, Carrouee B (2003) Considering pea in sustainable agriculture: agricultural and environmental arguments. Quelle place pour le pois dans une agriculture respectueuse de l'environnement? Argumentaire agri-environnemental. Cahiers Agric 12(2):111–120Google Scholar
- Naeem F, Malik KA, Hafeez FY (2008) Pisum sativum - Rhizobium interactions under different environmental stresses. Pak J Bot 40(6):2601–2612Google Scholar
- Ruffel S, Freixes S, Balzergue S, Tillard P, Jeudy C, Martin-Magniette ML, van der Merwe MJ, Kakar K, Gouzy J, Fernie AR, Udvardi M, Salon C, Gojon A, Lepetit M (2008) Systemic signaling of the plant nitrogen status triggers specific transcriptome responses depending on the nitrogen source in Medicago truncatula. Plant Physiol 146(4):2020–2035PubMedCentralPubMedCrossRefGoogle Scholar
- Sagan M, Duc G (1996) Sym28 and Sym29, two new genes involved in regulation of nodulation in pea (Pisum sativum L). Symbiosis 20(3):229–245Google Scholar
- Schnabel EL, Kassaw TK, Smith LS, Marsh JF, Oldroyd GE, Long SR, Frugoli JA (2011) The ROOT DETERMINED NODULATION1 gene regulates nodule number in roots of Medicago truncatula and defines a highly conserved, uncharacterized plant gene family. Plant Physiol 157(1):328–340. doi: 10.1104/pp. 111.178756 PubMedCentralPubMedCrossRefGoogle Scholar