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Plant and Soil

, Volume 376, Issue 1–2, pp 433–443 | Cite as

AtMYB20 is negatively involved in plant adaptive response to drought stress

  • Shuai Gao
  • Yong Li Zhang
  • Lu Yang
  • Jian Bo Song
  • Zhi Min Yang
Regular Article

Abstract

Background and aims

MYB transcription factors play critical roles in plant development and stress responses. Our objective was to characterize a role of AtMYB20 (AT1G66230) in regulating the ABA-dependent adaptive response to desiccation stress in Arabidopsis.

Methods

Sequencing analysis revealed that there is a site located on the AtMYB20 transcript which is potentially base-paired by miR858. To avoid the possible cleavage, a vector with a miR858-resistant version of AtMYB20 under the CaMV 35S promoter (35S:m5AtMYB20) was constructed. The AtMYB20 knock-out mutant myb20 was applied to identifying AtMYB20 functions.

Results

While AtMYB20 was induced by high levels of NaCl, its expression was suppressed by desiccation and cold stresses and abscisic acid (ABA) treatment. Compared with wild-type, AtMYB20 over-expression (35S:m5AtMYB20) seedlings were susceptible to desiccation, whereas MYB20 loss of function mutant myb20 plants displayed resistance to desiccation stress. 35S:m5AtMYB20 plants were less sensitive to ABA, but myb20 mutants were hypersensitive to ABA. This could be validated by the experiment in which treatment with 10 μM ABA for 2 h resulted in constant stomatal opening on leaves of 35S:m5AtMYB20 plants but stomatal closure on myb20 mutant plants. Expression of ABA- and drought stress-responsive marker genes (e.g. ABI3-5 and ABF3-4) was up-regulated in myb20 plants but down-regulated in 35S:m5AtMYB20 plants.

Conclusions

AtMYB20 acts as a negative regulator of plant response to desiccation stress in an ABA-dependent manner.

Keywords

Arabidopsis AtMYB20 Desiccation Abscisic acid 

Notes

Acknowledgments

This research was supported by The National Research Foundation for the Doctoral Program of Higher Education of China under Grant No B0201100671.

Supplementary material

11104_2013_1992_MOESM1_ESM.doc (34 kb)
Supplementary Data 1A Primer sequences used for PCR and semi-quantitative RT-PCR (DOC 34 kb)
11104_2013_1992_MOESM2_ESM.doc (38 kb)
Supplementary Data 1B Primer sequences used for quantitative real-time RT-PCR (DOC 37 kb)

References

  1. Abe H, Urao T, Ito T, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2003) Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling. Plant Cell 15:63–78PubMedCentralPubMedCrossRefGoogle Scholar
  2. Bartels D, Sunkar R (2005) Drought and salt tolerance in plants. Crit Rev Plant Sci 24:23–58CrossRefGoogle Scholar
  3. Bouchabke O, Chang F, Simon M, Voisin R, Pelletier G, Durand-Tardif M (2008) Natural variation in Arabidopsis thaliana as a tool for highlighting differential drought responses. PloS One 3:e1705PubMedCentralPubMedCrossRefGoogle Scholar
  4. Cedroni ML, Cronn RC, Adams KL, Wilkins TA, Wendel JF (2003) Evolution and expression of MYB genes in diploid and polyploidy cotton. Plant Mol Biol 51:313–332PubMedCrossRefGoogle Scholar
  5. Chen H, Li Z, Xiong L (2012) A plant microRNA regulates the adaptation of roots to drought stress. FEBS Lett 586:1742–1747PubMedCrossRefGoogle Scholar
  6. Chen YH, Yang XY, He K, Liu MH, Li JG, Gao ZF, Lin ZQ, Zhang YF, Wang XX, Qiu XM, Shen YP, Zhang L, Deng XH, Luo JC, Deng XW, Chen ZL, Gu HY, Qu LJ (2006) The MYB transcription factor superfamily of Arabidopsis: expression analysis and phylogenetic comparison with the rice MYB family. Plant Mol Biol 60:107–124CrossRefGoogle Scholar
  7. Cominelli E, Galbiati M, Vavasseur A, Conti L, Sala T, Vuylsteke M, Leonhardt N, Dellaporta SL, Tonelli C (2005) A guard-cell-specific MYB transcription factor regulates stomatal movements and plant drought tolerance. Curr Biol 15:1196–1200PubMedCrossRefGoogle Scholar
  8. Clough SJ, Bent AF (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16:735–743PubMedCrossRefGoogle Scholar
  9. Cui MH, Yoo KS, Hyoung S, Nguyen HTK, Kim YY, Kim HJ, Ok SH, Yoo SD, Shin JS (2013) An Arabidopsis R2R3-MYB transcription factor, AtMYB20, negatively regulates type 2C serine/threonine protein phosphatases to enhance salt tolerance. FEBS Lett 587:1773–1778PubMedCrossRefGoogle Scholar
  10. Cutler SR, Rodriguez PL, Finkelstein RR, Abrams SR (2010) Abscisic acid, emergence of a core signaling network. Annu Rev Plant Biol 61:651–679PubMedCrossRefGoogle Scholar
  11. Ding Z, Li S, An X, Liu X, Qin H, Wang D (2009) Transgenic expression of MYB15 confers enhanced sensitivity to abscisic and improved drought tolerance in Arabidopsis thaliana. J Genet Genomics 36:17–29PubMedCrossRefGoogle Scholar
  12. Du Z, Xu D, Li L, Yao S, Schlappi M, Xu ZQ (2012) Inhibitory effects of Arabidopsis EARLI1 against Botrytis cinerea and Bradysia difformis. Plant Cell Tissue Organ Cult 110:435–443CrossRefGoogle Scholar
  13. Dubos C, Stracke R, Grotewold E, Weisshaar B, Martin C, Lepiniec L (2010) MYB transcription factors in Arabidopsis. Trend Plant Sci 15:573–581CrossRefGoogle Scholar
  14. German MA, Pillay M, Jeong DH, Hetawal A, Luo S, Janardhanan P, Kannan V, Rymarquis LA, Nobuta K, German R, De Paoli E, Lu C, Schroth G, Meyers BC, Green PJ (2008) Global identification of microRNA–target RNA pairs by parallel analysis of RNA ends. Nat Biotechnol 26:941–946PubMedCrossRefGoogle Scholar
  15. Guo K, Xia K, Yang ZM (2008) Regulation of tomato lateral root development by carbon monoxide and involvement in auxin and nitric oxide. J Exp Bot 59:3443–3452PubMedCrossRefGoogle Scholar
  16. Hauser F, Waadt R, Schroeder JI (2011) Evolution of abscisic acid synthesis and signaling mechanisms. Curr Biol 21:R346–R355PubMedCentralPubMedCrossRefGoogle Scholar
  17. Jung C, Seo JS, Han SW, Koo YJ, Kim CH, Song SI, Nahm BH, Choi YD, Cheong JJ (2008) Overexpression of AtMYB44 enhances stomatal closure to confer abiotic stress tolerance in transgenic Arabidopsis. Plant Physiol 146:623–635PubMedCentralPubMedCrossRefGoogle Scholar
  18. Khraiwesh B, Zhu JK, Zhu JH (2012) Role of miRNAs and siRNAs in biotic and abiotic stress responses of plants. Biochim Biophys Acta 1819:137–148PubMedCentralPubMedCrossRefGoogle Scholar
  19. Kreps JA, Wu Y, Chang HS, Zhu T, Wang X, Harper JF (2002) Transcriptome changes for Arabidopsis in response to salt, osmotic, and cold stress. Plant Physiol 130:2129–2141PubMedCentralPubMedCrossRefGoogle Scholar
  20. Kuhn JM, Boisson-Dernier A, Dizon MB, Maktabi MH, Schroeder JI (2006) The protein phosphatase AtPP2CA negatively regulates abscisic acid signal transduction in Arabidopsis, and effects of abh1 on AtPP2CA mRNA. Plant Physiol 140:127–139PubMedCentralPubMedCrossRefGoogle Scholar
  21. Lemichez E, Wu Y, Sanchez JP, Mettouchi A, Mathur J, Chua NH (2001) Inactivation of AtRac1 by abscisic acid is essential for stomatal closure. Genet Dev 15:1808–1816CrossRefGoogle Scholar
  22. Li W, Cui X, Meng Z, Huang X, Wu H, Jin H, Zhang D, Liang W (2012) Transcriptional regulation of Arabidopsis MIR168a and ARGONAUTE1 homeostasis in Abscisic acid and abiotic stress responses. Plant Physiol 158:1279–1292PubMedCentralPubMedCrossRefGoogle Scholar
  23. Liu HX, Zhou X, Dong N, Liu X, Zhang HY, Zhang ZY (2011) Expression of a wheat MYB gene in transgenic tobacco enhances resistance to Ralstonia solanacearum, and to drought and salt stresses. Funct Integr Genomics 11:431–443PubMedCrossRefGoogle Scholar
  24. Rahaie M, Xue GP, Naghavi MR, Alizadeh H, Schenk PM (2010) A MYB gene from wheat (Triticum aestivum L.) is up-regulated during salt and drought stresses and differentially regulated between salt-tolerant and sensitive genotypes. Plant Cell Rep 29:835–844PubMedCrossRefGoogle Scholar
  25. Riechmann JL, Heard J, Martin G, Reuber L, Jiang CZ, Keddie J, Adam L, Pineda O, Ratcliffe OJ, Samaha RR, Creelman R, Pilgrim M, Broun P, Zhang JZ, Ghandehari D, Sherman BK, Yu G (2000) Arabidopsis transcription factors: genome-wide comparative analysis among eukaryote. Science 290:2105–2110PubMedCrossRefGoogle Scholar
  26. Reyes JL, Chua NH (2007) ABA induction of miR159 controls transcript levels of two MYB factors during Arabidopsis seed germination. Plant J 49:592–606PubMedCrossRefGoogle Scholar
  27. Schweighofer A, Hirt H, Meskiene I (2004) Plant PP2C phosphatases: emerging functions in stress signalling. Trends Plant Sci 9:236–243PubMedCrossRefGoogle Scholar
  28. Seo PJ, Xiang F, Qiao M, Park JY, Lee YN, Kin SG, Lee YH, Park WJ, Park CM (2009) The MYB96 transcription factor mediates abscisic acid signaling during drought stress response in Arabidopsis. Plant Physiol 151:275–289PubMedCentralPubMedCrossRefGoogle Scholar
  29. Shan H, Chen S, Jiang J, Chen F, Chen Y, Gu C, Li P, Song A, Zhu X, Gao H, Zhou G, Li T, Yang X (2012) Heterologous expression of the Chrysanthemun R2R3-MYB transcription factor CmMYB2 enhances drought and salinity tolerance, increases hypersensitivity to ABA and delays flowering in Arabidopsis thaliana. Mol Biotechnol 51:160–173PubMedCrossRefGoogle Scholar
  30. Shen Q, Jiang M, Li H, Che LL, Yang ZM (2011) Expression of a Brassica napus heme oxygenase confers plant tolerance to mercury toxicity. Plant Cell Environ 34:752–763PubMedCrossRefGoogle Scholar
  31. Shinozaki K, Yamaguchi-Shinozaki K, Seki M (2003) Regulatory network of gene expression in the drought and cold stress responses. Curr Opin Plant Biol 6:410–417PubMedCrossRefGoogle Scholar
  32. Singh K, Foley RC, Onate-Sanchez L (2002) Transcription factors in plant defense and stress responses. Curr Opin Plant Biol 5:430–436PubMedCrossRefGoogle Scholar
  33. Song JB, Huang SQ, Dalmay T, Yang ZM (2012) Regulation of leaf morphology by microRNA394 and its target LEAF CURLING RESPONSIVENESS. Plant Cell Physiol 53:1283–1294PubMedCrossRefGoogle Scholar
  34. Sreenivasulu N, Sopory SK, Kishor PBK (2007) Deciphering the regulatory mechanisms of abiotic stress tolerance in plants by genomic approaches. Gene 388:1–13PubMedCrossRefGoogle Scholar
  35. Winter D, Vinegar B, Nahal H, Ammar R, Wilson GV, Provart NJ (2007) An “Electronic Fluorescent Pictograph” browser for exploring and analyzing large-scale biological data sets. PLoS One 8:e718CrossRefGoogle Scholar
  36. Yamaguchi-Shinozaki K, Shinozaki K (1993) The plant hormone abscisic acid mediates the drought-induced expression but not the seed-specific expression of rd22, a gene responsive to desiccation stress in Arabidopsis thaliana. Mol Gen Genet 238:17–25PubMedGoogle Scholar
  37. Yang ZM, Chen J (2013) A potential role of microRNAs in regulating plant response to metal toxicity. Metallomics 5:1184–1190PubMedCrossRefGoogle Scholar
  38. Yoshida T, Nishimura N, Kitahata N, Kuromori T, Ito T, Asami T, Shinozaki K, Hirayama T (2006) ABA-hypersensitive germination3 encodes a protein phosphatase 2C (AtPP2CA) that strongly regulates abscisic acid signaling during germination among Arabidopsis protein phosphatase 2Cs. Plant Physiol 140:115–126PubMedCentralPubMedCrossRefGoogle Scholar
  39. Zhang Y, Yang C, Li Y, Zheng N, Chen H, Zhao Q, Gao T, Guo H, Xie Q (2007) SDIR1 is a RINH finger E3 ligase that positively regulates stress-responsive abscisic acid signaling in Arabidopsis. Plant Cell 19:1912–1929PubMedCentralPubMedCrossRefGoogle Scholar
  40. Zhao J, Gao Y, Zhang Z, Chen T, Guo W, Zhang T (2013) A receptor-like kinase gene (GbRLK) from Gossypium barbadense enhances salinity and drought-stress tolerance in Arabidopsis. BMC Plant Biol 13:110PubMedCentralPubMedCrossRefGoogle Scholar
  41. Zhou ZS, Song JB, Yang ZM (2012) Genome-wide identification of Brassica napus microRNAs and their targets reveals their differential regulation by cadmium. J Exp Bot 59:3443–3452Google Scholar
  42. Zhou ZS, Yang SN, Li H, Zhu CC, Liu ZP, Yang ZM (2013) Molecular dissection of mercury-responsive transcriptome and sense/antisense genes in Medicago truncatula by high-throughput sequencing. J Hazard Mater 252–253:123–131PubMedCrossRefGoogle Scholar
  43. Zhu JK (2002) Salt and drought stress signal transduction in plants. Annu Rev Plant Biol 53:247–273PubMedCentralPubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Shuai Gao
    • 1
  • Yong Li Zhang
    • 1
  • Lu Yang
    • 1
  • Jian Bo Song
    • 1
  • Zhi Min Yang
    • 1
  1. 1.Weigang No.1, Department of Biochemistry and Molecular Biology, College of Life ScienceNanjing Agricultural UniversityNanjingChina

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