Alleviation of drought stress in mung bean by strain Pseudomonas aeruginosa GGRJ21
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Backgrounds and aims
Mung bean (Vigna radiata (L.) R. Wilczek), a widely cultivated pulse crops in India, experiences severe drought stress during the cultivation period. Apart from the conventional plant breeding and transgenic approaches, the application of plant growth-promoting rhizobacteria (PGPR) has always been a promising approach to improve abiotic stress tolerance in crop plants. The aim of the present study was to investigate the role of mung bean rhizosphere-associated Pseudomonas aeruginosa GGRJ21 strain on drought stress alleviation in the host plant.
Fluorescent pseudomonads were isolated from mung bean rhizosphere by employing a culture-dependent approach. The role of osmotic stress tolerant P. aeruginosa GGRJ21 on drought stress alleviation in host plants was further examined in both the green house and field conditions.
An elevated production of reactive oxygen species scavenging enzymes and cellular osmolytes; increased root length, shoot length, dry weight, relative water content; and a stronger upregulation of three drought stress-responsive genes, i.e., dehydration-responsive element binding protein (DREB2A), catalase (CAT1), and dehydrin (DHN) were observed in GGRJ21 inoculated plants in comparison with the uninoculated control plants tested under drought conditions. The field experimental data show an increase in biomass and better growth and development in inoculated and stressed plants when compared with untreated and stressed plants.
P. aeruginosa GGRJ21 strain was found to elicit water stress tolerance in mung bean plants by accelerating the accumulation of inherent levels of antioxidant enzymes, cell osmolytes, and consistently expediting the upregulation of stress responsive genes in PGPR-treated plants under water stress conditions.
KeywordsDrought stress Pseudomonas aeruginosa Catalase Peroxidase Superoxide dismutase Proline Reactive oxygen species
The work is supported by a Network Project (AMAAS), sponsored by the Indian Council of Agricultural Research (ICAR), the Government of India, New Delhi. The authors are thankful to the Director, CSIR-NEIST, and the Head, Biotechnology Division, CSIR-NEIST, for providing necessary facilities to carry out the work. The authors are also thankful to the MAEP Division, CSIR-NEIST for providing necessary area in experimental farm to conduct the field experiment.
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