N- and P-mediated seminal root elongation response in rice seedlings
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In rice, seminal root elongation plays an important role in acquisition of nutrients such as N and P, but the extent to which different N forms and P concentrations affect root growth is poorly understood. This study aimed to examine N- and P-mediated seminal root elongation response and to identify putative QTLs associated with seminal root elongation.
Seminal root elongation was evaluated in 15 diverse wild and cultivated accessions of rice, along with 48 chromosome segment substitution lines (CSSLs) derived from a cross between the rice variety ‘Curinga’ and Oryza rufipogon (IRGC 105491). Root elongation in response to different forms of N (NH4 +, NO3 − and NH4NO3) and concentrations of P was evaluated under hydroponic conditions, and associated putative QTL regions were identified.
The CSSL parents had contrasting root responses to N and P. Root elongation in O. rufipogon was insensitive to N source and concentration, whereas Curinga was responsive. In contrast to N, seminal root elongation and P concentration was positively correlated. Three putative QTLs for seminal root elongation in response to N were detected on chromosome 1, and one QTL on chromosome 3 was associated with low P concentration.
Genetic variation in seminal root elongation and plasticity of nutrient response may be appropriate targets for marker-assisted selection to improve rice nutrient acquisition efficiency.
KeywordsChromosome segment substitution lines NH4+ response N acquisition Seminal root elongation
Chromosome segment substitution line
Nitrogen acquisition efficiency
Partial nitrate nutrition
Phosphorus acquisition efficiency
Quantitative trait locus
Single nucleotide polymorphism
Simple sequence repeat
We thank Dr. Edgar Torres (CIAT, Colombia) and Dr. Uga Y (National Institute of Agrobiological Sciences, Japan) for providing the seed materials used this study, and Dr. Joe Tohme, CIAT Agrobiodiversity Research Area Director, for his continuous support. We also thank Dr. T. Ramasubramanian (Sugarcane Breeding Institute, Indian Council of Agricultural Research, Tamil Nadu, India) for his critical evaluation of the manuscript, and are grateful for the assistance of Lucia Chavez and Milton Valencia.
This work was supported by Ministry of foreign Affairs of Japan.
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