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Plant and Soil

, Volume 373, Issue 1–2, pp 699–710 | Cite as

Do arbuscular mycorrhizas or heterotrophic soil microbes contribute toward plant acquisition of a pulse of mineral phosphate?

  • Nazanin K. Nazeri
  • Hans Lambers
  • Mark Tibbett
  • Megan H. Ryan
Regular Article

Abstract

Aims

We investigated the role of arbuscular mycorrhizal fungi (AMF) and heterotrophic soil microbes in the uptake of phosphorus (P) by Trifolium subterraneum from a pulse.

Methods

Plants were grown in sterilised pasture field soil with a realistic level of available P. There were five treatments, two of which involved AMF: 1) unsterilised field soil containing a community of AMF and heterotrophic organisms; 2) Scutellospora calospora inoculum (AMF); 3) microbes added as filtrate from the field soil; 4) microbes added as filtrate from the S. calospora inoculum; 5) no additions, i.e. sterilised field soil. After 11 weeks, plants were harvested: 1 day before (day 0), 1 day after (day 2) and 7 days after (day 8) the pulse of P (10 mg kg−1).

Results

There was no difference among treatments in shoot and root dry weight, which increased from day 0 to day 8. At day 0, shoots and roots of plants in the colonised treatments had higher P and lower Mn concentrations. After the pulse, the rate of increase in P concentration in the shoots was slower for the colonised plants, and the root Mn concentration declined by up to 50 % by day 2.

Conclusions

Plants colonised by AMF had a lower rate of increase in shoot P concentration after a pulse, perhaps because intraradical hyphae accumulated P and thus reduced its transport to the shoots.

Keywords

Arbuscular mycorrhizal fungi Heterotrophic soil microbes Manganese Phosphorus pulse Scutellospora calospora Shoot and root nutrient concentrations 

Notes

Acknowledgments

This project was funded by the Future Farm Industries CRC. We wish to thank Tamara Edmonds-Tibbett for technical support of the preliminary experiment.

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Nazanin K. Nazeri
    • 1
    • 2
    • 5
  • Hans Lambers
    • 1
  • Mark Tibbett
    • 2
    • 3
    • 4
  • Megan H. Ryan
    • 1
    • 2
  1. 1.School of Plant Biology and Institute of AgricultureThe University of Western AustraliaCrawleyAustralia
  2. 2.Future Farm Industries Cooperative Research CentreThe University of Western AustraliaCrawleyAustralia
  3. 3.School of Earth and EnvironmentThe University of Western AustraliaCrawleyAustralia
  4. 4.National Soil Resources Institute, Department of Environmental Science and Technology, School of Applied SciencesCranfield UniversityCranfieldUK
  5. 5.School of Plant Biology, M080The University of Western AustraliaCrawleyAustralia

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