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Plant and Soil

, Volume 367, Issue 1–2, pp 673–685 | Cite as

Burkholderia phymatum improves salt tolerance of symbiotic nitrogen fixation in Phaseolus vulgaris

  • Chouhra Talbi
  • Montserrat Argandoña
  • Manuel Salvador
  • Juan D. Alché
  • Carmen Vargas
  • Eulogio J. Bedmar
  • María J. Delgado
Regular Article

Abstract

Background and aims

Burkholderia phymatum strain GR01 is a recently reported common bean (Phaseolus vulgaris) symbiont isolated from nodules of plants grown in semi-arid soils in Morocco. The osmotolerance of B. phymatum GR01N under free-living and in symbiotic association with P. vulgaris was investigated in this study.

Methods

The osmotolerance of B. phymatum GR01N was checked by growing cells in the presence of varying concentrations of NaCl or sucrose, and the cellular solutes were analyzed in cell extracts by 13C-nuclear magnetic resonance (NMR) spectroscopy. Nodule occupancy was checked in P. vulgaris grown in the presence of 25, 35 or 50 mM NaCl and inoculated with a mixture of B. phymatum GR01N and R. tropici CIAT899R cells. The effect of salt stress on nodule biomass, plant dry weight, plant nitrogen content and leghaemoglobin content of nodules was also analyzed in plants inoculated with either B. phymatum GR01N or R. tropici CIAT899R and grown in the presence of 25 or 35 mM NaCl.

Results

Burkholderia phymatum strain GR01N showed increased tolerance to osmotic stress under free-living conditions as compared to the reference strain R. tropici CIAT899R. Strain GR01N accumulated trehalose, mannitol and alanine in response to saline stress, suggesting their role in the observed osmoloterance. Under conditions of saline stress, P. vulgaris plants nodulated by B. phymatum GR01N showed increased plant dry weight and nitrogen fixation, when compared to those inoculated with R. tropici CIAT899R. Nodule competition assays revealed that B. phymatum GR01N had higher levels of nodule occupancy than R. tropici CIAT899R in P. vulgaris plants grown under saline conditions.

Conclusions

Burkholderia phymatum strain GR01N displays a remarkable osmotolerance under free-living and symbiotic conditions.

Keywords

Common bean Nodules Osmotolerance Rhizobium tropici 13C-NMR spectroscopy 

Notes

Acknowledgments

This work was supported by a European Regional Development Fund (ERDF)-cofinanced grant CVI-3177 from Junta de Andalucıa (Spain) and grant AGL2010-18607 from Ministerio de Economia y Competitividad (Spain). Grant 107PICO312 from Programa Iberoamericano de Ciencia y Tecnologıa para el Desarrollo (CYTED) and support from Junta de Andalucía to Group BIO-275 is also acknowledged.

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Chouhra Talbi
    • 1
  • Montserrat Argandoña
    • 2
  • Manuel Salvador
    • 2
  • Juan D. Alché
    • 3
  • Carmen Vargas
    • 2
  • Eulogio J. Bedmar
    • 1
  • María J. Delgado
    • 1
  1. 1.Department of Soil Microbiology and Symbiotic SystemsEstación Experimental del Zaidin, CSICGranadaSpain
  2. 2.Departamento de Microbiología y ParasitologíaUniversidad de SevillaSevilleSpain
  3. 3.Department of Biochemistry, Cellular and Molecular Plant BiologyEstación Experimental del Zaidin, CSICGranadaSpain

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