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Plant and Soil

, Volume 364, Issue 1–2, pp 261–272 | Cite as

Successional changes in the soil microbial community along a vegetation development sequence in a subalpine volcanic desert on Mount Fuji, Japan

  • Shinpei Yoshitake
  • Masaaki Fujiyoshi
  • Kenichi Watanabe
  • Takehiro Masuzawa
  • Takayuki Nakatsubo
  • Hiroshi Koizumi
Regular Article

Abstract

Aims

To study the relationship between vegetation development and changes in the soil microbial community during primary succession in a volcanic desert, we examined successional changes in microbial respiration, biomass, and community structure in a volcanic desert on Mount Fuji, Japan.

Methods

Soil samples were collected from six successional stages, including isolated island-like plant communities. We measured microbial respiration and performed phospholipid fatty acid (PLFA) analysis, denaturing gradient gel electrophoresis (DGGE) analysis, and community-level physiological profile (CLPP) analysis using Biolog microplates.

Results

Microbial biomass (total PLFA content) increased during plant succession and was positively correlated with soil properties including soil water and soil organic matter (SOM) contents. The microbial respiration rate per unit biomass decreased during succession. Nonmetric multidimensional scaling based on the PLFA, DGGE, and CLPP analyses showed a substantial shift in microbial community structure as a result of initial colonization by the pioneer herb Polygonum cuspidatum and subsequent colonization by Larix kaempferi into central areas of island-like communities. These shifts in microbial community structure probably reflect differences in SOM quality.

Conclusions

Microbial succession in the volcanic desert of Mt. Fuji was initially strongly affected by colonization of the pioneer herbaceous plant (P. cuspidatum) associated with substantial changes in the soil environment. Subsequent changes in vegetation, including the invasion of shrubs such as L. kaempferi, also affected the microbial community structure.

Keywords

Island-like plant community Microbial biomass Microbial community structure Primary succession Volcanic desert 

Notes

Acknowledgements

We thank Dr N. Sakurai of Hiroshima University for providing the laboratory facilities. The comments from the editor and four anonymous reviewers greatly improved the manuscript. This study was partly supported by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science.

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Shinpei Yoshitake
    • 1
  • Masaaki Fujiyoshi
    • 2
  • Kenichi Watanabe
    • 3
  • Takehiro Masuzawa
    • 4
  • Takayuki Nakatsubo
    • 5
  • Hiroshi Koizumi
    • 6
  1. 1.Faculty of Science and EngineeringWaseda UniversityTokyoJapan
  2. 2.School of Humanities and CultureTokai UniversityHiratsukaJapan
  3. 3.National Institute of Polar ResearchTachikawaJapan
  4. 4.Graduate School of Science and Technology, Shizuoka UniversityShizuokaJapan
  5. 5.Graduate School of Biosphere Science, Hiroshima UniversityHiroshimaJapan
  6. 6.Faculty of Education and Integrated Arts and SciencesWaseda UniversityTokyoJapan

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