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Plant and Soil

, Volume 351, Issue 1–2, pp 121–134 | Cite as

Aluminum resistance mechanisms in oat (Avena sativa L.)

  • Lorien Radmer
  • Mesfin Tesfaye
  • David A. Somers
  • Stephen J. Temple
  • Carroll P. Vance
  • Deborah A. Samac
Regular Article

Abstract

Background and aims

Enhanced aluminum (Al) resistance has been observed in dicots over-expressing enzymes involved in organic acid synthesis; however, this approach for improving Al resistance has not been investigated in monocots. Among the cereals, oat (Avena sativa L.) is considered to be Al resistant, but the basis of resistance is not known.

Methods

A hydroponic assay and hematoxylin staining for Al accumulation in roots were used to evaluate Al resistance in 15 oat cultivars. Malate and citrate release from roots was measured over a 24 h period. A malate dehydrogenase gene, neMDH, from alfalfa (Medicago sativa L.) was used to transform oat.

Results

Oat seedlings were highly resistant to Al, as a concentration of 325 μM AlK(SO4)2 was needed to cause a 50% decrease in root growth. Most oat cultivars tested are naturally resistant to high concentrations of Al and effectively excluded Al from roots. Al-dependent release of malate and Al-independent release of citrate was observed. Al resistance was enhanced in a transgenic oat line with the highest accumulation of neMDH protein. However, overall root growth of this line was reduced and expression of neMDH in transgenic oat did not enhance malate secretion.

Conclusions

Release of malate from oat roots was associated with Al resistance, which suggests that malate plays a role in Al resistance of oat. Over-expression of alfalfa neMDH enhanced Al resistance in some lines but was not effective alone for crop improvement.

Keywords

Aluminium Aluminum resistance Avena sativa Citrate Malate dehydrogenase Malate secretion Oat Sugarcane bacilliform badnavirus (ScBV) promoter 

Notes

Acknowledgements

Mention of any trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture. This paper is a joint contribution from the Plant Science Research Unit, USDA-ARS, and the Minnesota Agricultural Experiment Station. We gratefully acknowledge the assistance of Kim Torbert for production of transformed oat plants, Deon Stuthman and Roger Caspars for oat seed, and Karen Hilburn for assistance with lyophilization.

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Copyright information

© Springer Science+Business Media B.V. (outside the USA) 2011

Authors and Affiliations

  • Lorien Radmer
    • 1
  • Mesfin Tesfaye
    • 1
  • David A. Somers
    • 2
    • 5
  • Stephen J. Temple
    • 2
    • 6
  • Carroll P. Vance
    • 3
  • Deborah A. Samac
    • 4
  1. 1.Department of Plant BiologyUniversity of MinnesotaSt. PaulUSA
  2. 2.Department of Agronomy and Plant GeneticsUniversity of MinnesotaSt. PaulUSA
  3. 3.USDA-ARS-Plant Science Research Unit and Department of Agronomy and Plant GeneticsUniversity of MinnesotaSt. PaulUSA
  4. 4.USDA-ARS-Plant Science Research Unit and Department of Plant PathologyUniversity of MinnesotaSt. PaulUSA
  5. 5.Monsanto CompanyChesterfieldUSA
  6. 6.Forage Genetics InternationalWest SalemUSA

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