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Plant and Soil

, Volume 345, Issue 1–2, pp 89–102 | Cite as

Trigonella arcuata-associated rhizobia—an Ensifer (Sinorhizobium) meliloti population adapted to a desert environment

  • Yu Rong He
  • Jing Yu Wang
  • En Tao Wang
  • Gu Feng
  • Yue Li Chang
  • Xin Hua Sui
  • Wen Xin Chen
Regular Article

Abstract

With the aim of systematically investigating rhizobia associated with the Trigonella arcuata—an ephemeral legume native to the Gurbantunggut Desert—66 bacterial strains were isolated from root nodules and characterized with PCR-based RFLP of 16S rRNA and nodC genes, RFLP of ribosomal IGS, BOX-PCR, and sequencing of housekeeping (16S rRNA, recA, atpD) and symbiotic (nodC and nifH) genes, numerical taxonomy and nodulation tests. The T. arcuata rhizobia were classified as Ensifer (Sinorhizobium) meliloti, but the 10% differences in nodC and nifH gene sequences and the 20% phenotypic dissimilarity demonstrated that the T. arcuata rhizobia have divergently evolved from the E. meliloti reference strains. Although they shared several other hosts under laboratory conditions, the studied rhizobial population seems adapted to their natural environment based upon their wide range of resistance to desert conditions. All these data indicate that T. arcuata rhizobia are a valuable source of rhizobial inoculants for bioremediation of arid areas.

Keywords

Ephemeral plant Trigonella arcuata Desert Rhizobia Divergent evolution 

Notes

Acknowledgments

The authors would like to thank Dr. Michael Dunn for critical reading the manuscript. This work was supported by the Foundation of the National Project for Basic S & T Platform Construction (grant 2005DKA21201-10), of the State Key Basic Research and Development Plan of China (grant 2010CB126500), and by National Natural Science Foundation of China (project no. 30970004). ETW was financially supported by grants of SIP20100067 authorized by IPN and PICS08-3 authorized by ICyT DF of Mexico.

Supplementary material

11104_2011_762_MOESM1_ESM.ppt (174 kb)
ESM 1 (PPT 174 kb)
11104_2011_762_MOESM2_ESM.doc (81 kb)
Supplementary Table S1 Phenotypic features of Tragonalla arcuata rhozobia and its closest relatives. (DOC 81.0 kb)

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Yu Rong He
    • 1
  • Jing Yu Wang
    • 1
  • En Tao Wang
    • 2
  • Gu Feng
    • 3
  • Yue Li Chang
    • 1
  • Xin Hua Sui
    • 1
  • Wen Xin Chen
    • 1
  1. 1.State Key Laboratory of AgroBiotechnology, College of Biological SciencesChina Agricultural UniversityBeijingChina
  2. 2.Departamento de Microbiología, Escuela Nacional de Ciencias BiológicasInstituto Politécnico NacionalMéxicoMexico
  3. 3.Key Laboratory of Plant Nutrition, Ministry of AgricultureChina Agricultural UniversityBeijingChina

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