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Plant and Soil

, Volume 343, Issue 1–2, pp 379–392 | Cite as

Arbuscular mycorrhizas in phosphate-polluted soil: interrelations between root colonization and nitrogen

  • Verena Blanke
  • Markus Wagner
  • Carsten Renker
  • Hannelore Lippert
  • Manfred Michulitz
  • Arnd J. Kuhn
  • François Buscot
Regular Article

Abstract

To investigate whether arbuscular mycorrhizal fungi (AMF) – abundant in a phosphate-polluted but nitrogen-poor field site – improve plant N nutrition, we carried out a two-factorial experiment, including N fertilization and fungicide treatment. Percentage of root length colonized (% RLC) by AMF and tissue element concentrations were determined for four resident plant species. Furthermore, soil nutrient levels and N effects on aboveground biomass of individual species were measured. Nitrogen fertilization lowered % RLC by AMF of Artemisia vulgaris L., Picris hieracioides L. and Poa compressa L., but not of Bromus japonicus Thunb. This – together with positive N addition effects on N status, N:P-ratio and aboveground biomass of most species – suggested that plants are mycorrhizal because of N deficiency. Fungicide treatment, which reduced % RLC in all species, resulted in lower N concentrations in A. vulgaris and P. hieracioides, a higher N concentration in P. compressa, and did not consistently affect N status of B. japonicus. Evidently, AMF had an influence on the N nutrition of plants in this P-rich soil; however – potentially due to differences in their mycorrhizal responsiveness – not all species seemed to benefit from a mycorrhiza-mediated N uptake and accordingly, N distribution.

Keywords

Arbuscular mycorrhiza Benomyl Element concentrations Nitrogen fertilization Phosphate pollution Root colonization 

Abbreviations

AM

Arbuscular mycorrhiza

AMF

Arbuscular mycorrhizal fungi

% RLC

Percentage of root length colonized

Notes

Acknowledgements

This work was supported by a grant from the German Research Foundation (GRK 266). We thank Sandra Schau and Boris Börstler for help in the field, Birgit Schulze for freeze-drying of plant samples at the MPI for Chemical Ecology in Jena, Nadine Merki and Karin Lühring for plant element analyses and Claudia Krüger for root staining. We also thank Christoph Scherber for help with statistical analyses, Karen Budge for improving the English of the manuscript and Stefan Hempel for reviewing the manuscript prior to submission. Further, we thank two anonymous reviewers for helpful comments. Scotts Deutschland GmbH provided the fertilizer for free.

Supplementary material

11104_2011_727_MOESM1_ESM.pdf (40 kb)
Online Resource 1 Tissue element concentrations (other than N, P and N:P) of Artemisia vulgaris, Picris hieracioides, Poa compressa and Bromus japonicus for the different treatment combinations (averaged across blocks) (PDF 39 kb)
11104_2011_727_MOESM2_ESM.pdf (21 kb)
Online Resource 2 Total soil element concentrations (other than N, P and pH) for the different treatment combinations (averaged across blocks) (PDF 20 kb)

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Verena Blanke
    • 1
    • 2
    • 7
  • Markus Wagner
    • 3
  • Carsten Renker
    • 2
    • 8
  • Hannelore Lippert
    • 4
  • Manfred Michulitz
    • 4
  • Arnd J. Kuhn
    • 5
  • François Buscot
    • 2
    • 6
  1. 1.Institute of EcologyFriedrich-Schiller-University JenaJenaGermany
  2. 2.Department of Soil EcologyUFZ - Helmholtz Centre for Environmental ResearchHalle/SaaleGermany
  3. 3.NERC Centre for Ecology & HydrologyWallingfordUK
  4. 4.Central Division of Analytical Chemistry (ZCH)Research Centre JülichJülichGermany
  5. 5.Institute for Phytosphere Research (ICG-3)Research Centre JülichLeo-Brandt-StrasseJülichGermany
  6. 6.Institute of Biology, Chair of Soil EcologyUniversity of LeipzigLeipzigGermany
  7. 7.Agroscope Reckenholz-Tänikon Research Station ART, Air Pollution/Climate GroupZürichSwitzerland
  8. 8.Natural History Museum MainzMainzGermany

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