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Plant and Soil

, Volume 335, Issue 1–2, pp 325–337 | Cite as

Comparative metabolic activity related to flavonoid synthesis in leaves and flowers of Chrysanthemum morifolium in response to K deficiency

  • Wei Liu
  • Duanwei Zhu
  • Dahui Liu
  • Xiao Lu
  • Mingjian Geng
Regular Article

Abstract

K limitation could decrease the flavonoids content in many Chinese traditional herbs. These results may be caused by the influence of K deficiency on the synthesis pathway of flavonoids. In this paper, we aim to study the influence of K deficiency on secondary metabolites and activities of phenylalanine ammonia lyase (PAL), 4-coumarate coenzyme A ligase (4CL) and cinnamate 4-hydroxylase (C4H) enzymes in the process of flavonoid synthesis in Chrysanthemum morifolium Ramat. The results show that K deficiency decreased the flavonoid and chlorogenic acid contents slightly in flower of C. morifolium while the same effect was obtained on C4H and PAL. Total flavonoids content increased with the content of cinnamic acid and p-coumaric acid in the plant under K deficiency, respectively. The regression equations between content of flavonoids and cinnamic acid/ p-coumaric acid should be expressed in term of linear effects (R2 = 0.9809, P < 0.001 for cinnamic acid and R2 = 0.9929, P < 0.0001 for p-coumaric acid, respectively). But the effect of phenylalanine on flavonoids should be expressed in term of quadratic pattern (R2 = 0.9375, P < 0.05). There were two kinds principal components from chorismate to coumaryl CoA synthesis process, principal 1 was the substrate (phenylalanine, cinnamic acid, p-coumaric acid and PAL) and principal 2 was the enzyme (4CL, C4H), the principal 1 was the domination principal in both K deficient (88.36% and 10.57%) and sufficient treatments (88.17% and 9.64%), however, the factor loading (correlation coefficient of measured targets and the corresponding principal component) in principal 1 was opposite under different K application.

Keywords

Chrysanthemum morifolium Ramat. Enzyme activity Secondary metabolism Potassium deficiency Flavonoid synthesis 

Notes

Acknowledgements

The authors would like to thank all the researchers in Laboratory of Plant Nutrition and Ecological Environment Research. Many people have made invaluable contributions, both directly and indirectly to our research. The work was supported by the Ministry of Science and Technology of Hubei Province, China (Project 2001AA304A).

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Wei Liu
    • 1
  • Duanwei Zhu
    • 1
  • Dahui Liu
    • 1
    • 2
  • Xiao Lu
    • 3
  • Mingjian Geng
    • 1
  1. 1.Laboratory of Plant Nutrition and Ecological Environment Research, Centre for Microelement Research of Huazhong Agricultural University, Key Laboratory of Subtropical Agriculture and EnvironmentChinese Ministry of AgricultureWuhanChina
  2. 2.Institute of Medicinal PlantsYunnan Academy of Agricultural SciencesKunmingChina
  3. 3.Central LaboratoryShandong Agricultural Academy of SciencesJinanChina

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