Plant and Soil

, Volume 333, Issue 1–2, pp 49–58 | Cite as

Morphological changes in the apex of pea roots during and after recovery from aluminium treatment

  • Hirotoshi Motoda
  • Yoshio Kano
  • Fukumi Hiragami
  • Kenji Kawamura
  • Hideaki Matsumoto
Regular Article


We investigated how the pea (Pisum sativum cv. Harunoka) root, upon return to an Al-free condition, recovers from injury caused by exposure to Al. The growing region of the root during and after treatment with Al was examined by marking the root at intervals with India ink. Al-induced cell death was detected by staining with Evans blue. Root growth in 40 μM Al solution relative to that in Al-free solution (RRG) was approximately 45% from 6 h to12 h after the start of the treatment. However, values of RRG from 12 h to 24 h in Al-free solution for recovery or in the same Al solution were about 75% and 35%, respectively, indicating recovery from Al-induced growth inhibition. Images of the root characterized by zonal staining with Evans blue were observed in the sub-apical region (more than 1 mm from the tip) in Al-stressed roots. However, the interval of the stained zone was widened in the root after recovery from Al-induced growth inhibition, though it was narrower and more densely stained with time in the Al-stressed roots. During the recovery, the root apex may resume elongation in a specified region without Al-induced death or injury in cells detected by Evans blue.


Al stress Inhibition Injury Pea Recovery Root apex 


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Hirotoshi Motoda
    • 1
  • Yoshio Kano
    • 1
  • Fukumi Hiragami
    • 1
  • Kenji Kawamura
    • 1
  • Hideaki Matsumoto
    • 1
  1. 1.Research Institute of Health and WelfareKIBI International UniversityTakahashi-cityJapan

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