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Plant and Soil

, Volume 329, Issue 1–2, pp 327–337 | Cite as

Salicylic acid-mediated hydrogen peroxide accumulation and protection against Cd toxicity in rice leaves

  • Yun-Yang Chao
  • Chao-Yeh Chen
  • Wen-Dar Huang
  • Ching Huei Kao
Regular Article

Abstract

The role of H2O2 in salicylic acid (SA)-induced protection of rice leaves against subsequent Cd toxicity was investigated. SA pretreatment resulted in an increase in the contents of endogenous SA, as judged by the expression of OsWRKY45 (a SA responsive gene), and H2O2 in rice leaves. Diphenyleneiodonium (DPI) and imidazole (IMD), inhibitors of NADPH oxidase, prevented SA-increased H2O2 production, suggesting that NADPH oxidase is a H2O2-generating enzyme in SA-pretreated rice leaves. DPI and IMD also inhibited SA-increased activities of superoxide dismutase (SOD), ascorbate peroixdase (APX), and glutathione reductase (GR) activities, but had no effect on SA-increased catalase (CAT) activity. Moreover, SA-induced protection against subsequent Cd toxicity could also be prevented by DPI and IMD. The inhibitory effect of DPI and IMD on SA-induced protection against subsequent Cd toxicity could be reversed by exogenous H2O2. All these results suggested that SA-induced protection against subsequent Cd toxicity is mediated through H2O2. This conclusion is supported further by the observations that exogenous H2O2 application resulted in an increase in SOD, APX, and GR activities, but not CAT activity and a protection against subsequent Cd toxicity of rice leaves.

Keywords

Cadmium Hydrogen peroxide Oryza sativa L. Oxidative stress Salicylic acid 

Abbreviations

APX

Ascorbate peroxidase

AsA

Ascorbate

CAT

Catalase

DAB

3,3-Diaminobenzidine

DPI

Diphenyleneiodonium

DW

Dry weight

FW

Initial fresh weight

GR

Glutathione reductase

IMD

Imidazole

IRT

Iron-regulated transporter

MDA

Malondialdehyde

ROS

Reactive oxygen species

SA

Salicylic acid

SOD

Superoxide dismutase

Notes

Acknowledgements

This work was supported by a research grant of the National Science Council of the Republic of China (NSC2628-B-002-002).

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Yun-Yang Chao
    • 1
  • Chao-Yeh Chen
    • 1
  • Wen-Dar Huang
    • 1
  • Ching Huei Kao
    • 1
  1. 1.Department of AgronomyNational Taiwan UniversityTaipeiRepublic of China

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