Plant and Soil

, Volume 316, Issue 1–2, pp 25–34 | Cite as

Genotypic differences in root hydraulic conductance of rice (Oryza sativa L.) in response to water regimes

Regular Article


To determine water uptake by rice in water-saving culture, we examined root hydraulic conductance (L 0), plant growth, and root anatomy of three rice genotypes (Oryza sativa L. ssp. indica cv. Beodien, traditional upland; ssp. japonica cv. Sensho, traditional upland; ssp. japonica cv. Koshihikari, improved lowland) under three water regimes: water-saturated (hydroponic), well-irrigated aerobic (control), and water-saving aerobic in soil. In hydroponic culture, although shoot dry weight (SDW) and root number were the largest in Sensho, root L 0 was the highest in Koshihikari. There was no significant relationship between root L 0 and SDW in hydroponics, so root L 0 might not limit shoot growth under flooding. Root L 0 was much less in soil than in hydroponics, and that of Koshihikari was the lowest, especially in water-saving conditions. Root L 0 was highly correlated with SDW under water-saving conditions but not in the control, so root L 0 limits shoot growth under repeated water stress. Root anatomy was less affected by water regime than root L 0 and is genetically controlled. Thus, root L 0 may be more affected by water channels than by root anatomy.


Hydroponic culture Lowland rice Root hydraulic conductance Soil culture Upland rice Water-saving culture 


  1. Amodeo G, Dorr R, Vallejo A, Atuka M, Parisi M (1999) Radial and axial water transport in sugar beet storage root. J Exp Bot 50:509–516 doi: 10.1093/jexbot/50.333.509 CrossRefGoogle Scholar
  2. Azaizeh H, Steudle E (1991) Effects of salinity on water transport of excised maize (Zea mays L.) roots. Plant Physiol 97:1136–1145PubMedCrossRefGoogle Scholar
  3. Bouman BAM (2001) Water-efficiency management strategies in rice production. Int Rice Res Notes 26:17–22Google Scholar
  4. Bouman BAM, Tuong TP (2001) Field water management to save water and increase its productivity in irrigated rice. Agr Water Manag 49:11–30 doi: 10.1016/S0378-3774(00)00128-1 CrossRefGoogle Scholar
  5. Bouman BAM, Peng S, Castaneda AR, Visperas RM (2005) Yield and water use of irrigated tropical aerobic rice systems. Agr Water Manage 74:87–105 doi: 10.1016/j.agwat.2004.11.007 CrossRefGoogle Scholar
  6. Carvajal M, Cooke DT, Clarkson DT (1996) Responses of wheat plants to nutrient deprivation may involve the regulation of water-channel function. Planta 199:372–381 doi: 10.1007/BF00195729 CrossRefGoogle Scholar
  7. Carvajal M, Martinez V, Alcaraz CF (1999) Physiological function of water channels as affected by salinity in roots of paprika pepper. Physiol Plantarum 105:95–101 doi: 10.1034/j.1399-3054.1999.105115.x CrossRefGoogle Scholar
  8. Carvajal M, Cerda A, Martinez V (2000) Does calcium ameliorate the negative effect of NaCl on melon root water transport by regulating aquaporin activity? New Phytol 145:439–447 doi: 10.1046/j.1469-8137.2000.00593.x CrossRefGoogle Scholar
  9. Henzler T, Steudle E (1995) Reversible closing of water channels in Chara internodes provides evidence for a composite transport model of the plasma membrane. J Exp Bot 46:199–209 doi: 10.1093/jxb/46.2.199 CrossRefGoogle Scholar
  10. Henzler T, Waterhouse RN, Smyth AJ, Carvajal M, Cooke DT, Schaffner AR et al (1999) Diurnal variations in hydraulic conductivity and root pressure can be correlated with the expression of putative aquaporins in the roots of Lotus japonicus. Planta 210:50–60 doi: 10.1007/s004250050653 PubMedCrossRefGoogle Scholar
  11. Johanson U, Karlsson M, Johanson I, Gustavsson S, Sjövall S, Fraysse L et al (2001) The complete set of genes encoding major intrinsic proteins in Arabidopsis provides a framework for a new nomenclature for major intrinsic proteins in plants. Plant Physiol 126:1358–1368 doi: 10.1104/pp.126.4.1358 PubMedCrossRefGoogle Scholar
  12. Kondo M, Aguilar A, Abe J, Morita S (2000) Anatomy of nodal roots in tropical upland and lowland rice varieties. Plant Prod Sci 3:437–445CrossRefGoogle Scholar
  13. Lian HR, Yu X, Ye Q, Ding X, Kitagawa Y, Kwak SS et al (2004) The role of aquaporin RWC3 in drought avoidance in rice. Plant Cell Physiol 45:481–489 doi: 10.1093/pcp/pch058 PubMedCrossRefGoogle Scholar
  14. Lian HL, Yu X, Lane D, Sin WN, Tang ZC, Su WA (2006) Upland rice and lowland rice exhibited different PIP expression under water deficit and ABA treatment. Cell Res 16:651–660 doi: 10.1038/ PubMedCrossRefGoogle Scholar
  15. Matsuo N, Nhan, DQ, Mochizuki T (2007a) Effect of water-saving irrigation on rice yield and its water productivity. Jpn J Crop Sci 76: Extra issue 44–45 (in Japanese)Google Scholar
  16. Matsuo N, Nhan DQ, Mochizuki T (2007b) Effect of deep tillage on growth and yield of rice cultivars grown under water deficit. J Fac Agr Kyushu Univ 52:331–336Google Scholar
  17. Maurel C (1997) Aquaporins and water permeability of plant membrane. Annu Rev Plant Physiol Plant Mol Biol 48:399–429 doi: 10.1146/annurev.arplant.48.1.399 PubMedCrossRefGoogle Scholar
  18. Miyamoto N, Steudle E, Hirasawa T, Lafitte R (2001) Hydraulic conductivity of rice roots. J Exp Bot 362:1835–1846 doi: 10.1093/jexbot/52.362.1835 CrossRefGoogle Scholar
  19. Mu Z, Zhang S, Zhang L, Liang A, Liang Z (2006) Hydraulic conductivity of whole root system is better than hydraulic conductivity of single root in correlation with the leaf water status of maize. Bot Stud (Taipei, Taiwan) 47:145–151Google Scholar
  20. Munns R (1993) Physiological processes limiting plant growth in saline soils: some dogmas and hypotheses. Plant Cell Environ 16:15–24 doi: 10.1111/j.1365-3040.1993.tb00840.x CrossRefGoogle Scholar
  21. Murai-Hatano M, Kuwagata T, Sakurai J, Nonami H, Ahamed A, Nagasuga K et al (2008) Effect of low root temperature on hydraulic conductivity of rice plants and the possible role of aquaporins. Plant Cell Physiol (in press)Google Scholar
  22. North GB, Nobel PS (2000) Heterogeneity in water availability alters cellular development and hydraulic conductivity along roots of a desert succulent. Ann Bot (Lond) 85:247–255 doi: 10.1006/anbo.1999.1026 CrossRefGoogle Scholar
  23. Quigley F, Rosenberg JM, Shachar-Hill Y, Bohnert HJ (2002) From genome to function: The Arabidopsis aquaporins. Genome Biol 3:1–17Google Scholar
  24. Radin JW (1990) Responses of transpiration and Hydraulic conductance to root temperature in nitrogen- and phosphorus-deficient cotton seedlings. Plant Physiol 92:855–857PubMedCrossRefGoogle Scholar
  25. Ranathunge K, Steudle E, Lafitte R (2003) Control of water uptake by rice (Oryza sativa L.): role of the outer part of the root. Planta 217:193–205PubMedGoogle Scholar
  26. Ranathunge K, Kotula L, Steudle E, Lafitte R (2004) Water permeability and reflection coefficient of the outer part of young rice roots are differently affected by closure of water channels (aquaporins) or blockage of apoplastic pores. J Exp Bot 396:433–447 doi: 10.1093/jxb/erh041 CrossRefGoogle Scholar
  27. Ranathunge K, Steudle E, Lafitte R (2005) Blockage of apoplastic bypass-flow of water in rice roots by insoluble salt precipitates analogous to a Pfeffer cell. Plant Cell Environ 28:121–133 doi: 10.1111/j.1365-3040.2004.01245.x CrossRefGoogle Scholar
  28. Sakurai J, Ishikawa F, Yamaguchi T, Uemura M, Maeshima M (2005) Identification of 33 rice aquaporin genes and analysis of their expression and function. Plant Cell Physiol 46:1568–1577 doi: 10.1093/pcp/pci172 PubMedCrossRefGoogle Scholar
  29. Steudle E, Frensch J (1996) Water transport in plants: role of the apoplast. Plant Soil 187:67–79 doi: 10.1007/BF00011658 CrossRefGoogle Scholar
  30. Tournaire-Roux C, Sutka M, Javot H, Gout E, Gerbeau P, Luu D et al (2003) Cytosolic pH regulates root water transport during anoxic stress. Nature 425:393–397 doi: 10.1038/nature01853 PubMedCrossRefGoogle Scholar
  31. Trillo N, Fernández RJ (2005) Wheat plant hydraulic properties under prolonged experimental drought: Stronger decline in root-system conductance than in leaf area. Plant Soil 277:277–284 doi: 10.1007/s11104-005-7493-5 CrossRefGoogle Scholar
  32. Tuzet A, Perrier A, Leuning R (2003) A coupled model of stomatal conductance, photosynthesis and transpiration. Plant Cell Environ 26:1097–1116 doi: 10.1046/j.1365-3040.2003.01035.x CrossRefGoogle Scholar
  33. Vandeleur R, Niemietz C, Tilbrook J, Tyerman SD (2005) Roles of aquaporins in root responses to irrigation. Plant Soil 274:141–161 doi: 10.1007/s11104-004-8070-z CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Naoki Matsuo
    • 1
  • Kiyoshi Ozawa
    • 2
  • Toshihiro Mochizuki
    • 3
  1. 1.Graduate School of Bioresource and Bioenvironmental SciencesKyushu UniversityFukuokaJapan
  2. 2.Tropical Agriculture Research FrontJapan International Research Center for Agricultural SciencesIshigakiJapan
  3. 3.Faculty of AgricultureKyushu UniversityFukuokaJapan

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