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Plant and Soil

, 314:221 | Cite as

Anaerobic metabolism in roots of Kentucky bluegrass in response to short-term waterlogging alone and in combination with high temperatures

  • Kehua Wang
  • Shaomin Bian
  • Yiwei Jiang
Regular Article

Abstract

Waterlogging often occurs simultaneously with high temperatures during summer. The objective of this study was to characterize anaerobic metabolism and transcript abundance of fermentative enzymes in roots of Kentucky bluegrass (Poa pratensis L.) in response to short-term waterlogging and high temperature stresses. Grasses were subjected to four treatments: (1) well-drained under normal temperature (20/15°C, day/night; control); (2) waterlogging under normal temperature (WL); (3) well-drained under high temperature (35/30°C, day/night; HT); and (4) waterlogging under high temperature (WL + HT). Greater reductions in leaf elongation rate and shoot dry mass were observed under WL + HT than either stress alone. Root water-soluble carbohydrate concentration decreased 45% and 46% at 3 and 5 days of WL + HT, respectively, compared to the control. At 5 days, activities of root alcohol dehydrogenase increased 93% and 90% and lactate dehydrogenase increased 95% and 98% under WL and WL + HT, respectively, compared to the control. Transcript abundance of ADH and PDC genes in the roots were not or only slightly shown under the control or HT conditions but were induced by WL or WL + HT, particularly under WL. The results indicated that short-term WL + HT induced root anaerobic metabolism in a similar way to WL alone but had more severe effects on leaf growth and root WSC than did WL in Kentucky bluegrass.

Keywords

Waterlogging tolerance Metabolic activity Perennial grass Poa pratensis L. 

Abbreviations

ADH

alcohol dehydrogenase

HT

high temperature

LDH

lactate dehydrogenase

LE

leaf elongation rate

PDC

pyruvate decarboxylase

SDM

shoot dry mass

WL

waterlogging

WL + HT

waterlogging under high temperature

WSC

water-soluble carbohydrate

WSP

water-soluble protein

Notes

Acknowledgements

This research was supported by the Midwest Regional Turfgrass Foundation of Purdue University. Special thanks go to Judy Santini for statistical assistance.

References

  1. Bergmeyer HU (1983) Methods of enzymatic analysis, vol II, III. Verlag Chemie, Weinheim, p 701Google Scholar
  2. Bewley JD, Black M (1978) Physiology and biochemistry of seeds in relation to germination, vol 1: development, germination and growth. Springer, New YorkGoogle Scholar
  3. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254PubMedCrossRefGoogle Scholar
  4. Burdick DM, Mendelssohn IA (1990) Relationship between anatomical and metabolic responses to soil waterlogging in the coastal grass Spartina patens. J Exp Bot 41:223–228CrossRefGoogle Scholar
  5. Buysse J, Merckx R (1993) An improved colorimetric method to quantify the sugar content of plant tissue. J Exp Bot 49:1361–1370Google Scholar
  6. Chen H, Qualls RG (2003) Anaerobic metabolism in roots of the seedlings of invasive exotic Lepidium latifolium. Environ Exp Bot 50:29–40Google Scholar
  7. Dennis ES, Dolferus R, Ellis M, Rahman M, Wu Y, Hoeren FU et al (2000) Molecular strategies for improving waterlogging tolerance in plants. J Exp Bot 51:89–97PubMedCrossRefGoogle Scholar
  8. Drew MC (1983) Plant injury and adaptation to oxygen deficiency in the root environment: a review. Plant Soil 75:179–199CrossRefGoogle Scholar
  9. Drew MC (1997) Oxygen deficiency and root metabolism: injury and acclimation under hypoxia and anoxia. Annu Rev Plant Physiol Plant Mol Biol 48:223–250PubMedCrossRefGoogle Scholar
  10. Germain V, Raymond P, Richard B (1997) Differential expression of two tomato lactate dehydrogenase genes in response to oxygen deficit. Plant Mol Biol 35:711–721PubMedCrossRefGoogle Scholar
  11. Goggin DE, Colmer TD (2007) Wheat genotypes show contrasting abilities to recover from anoxia in spite of similar anoxic carbohydrate metabolism. J Plant Physiol 164:1605–1611PubMedCrossRefGoogle Scholar
  12. Hendry GAF, Grime JP (1993) Methods in comparative plant ecology. A laboratory manual. Chapman and Hall, London, p 252Google Scholar
  13. Hoffman NE, Bent AF, Hanson AD (1986) Induction of lactate dehydrogenase isozymes by oxygen deficit in barley root tissue. Plant Physiol 82:658–663PubMedGoogle Scholar
  14. Huang B, Liu X, Fry JD (1998a) Shoot physiological responses of two bentgrass cultivars to high temperature and poor soil aeration. Crop Sci 38:1219–1224Google Scholar
  15. Huang B, Liu X, Fry JD (1998b) Effects of high temperature and poor soil aeration on growth and viability of creeping bentgrass. Crop Sci 38:1618–1622Google Scholar
  16. Ismond KP, Dolferus R, De Pauw M, Dennis ES, Good AG (2003) Enhanced low oxygen survival in Arabidopsis through increased metabolic flux in the fermentative pathway. Plant Physiol 132:1292–1302PubMedCrossRefGoogle Scholar
  17. Jiang Y, Huang B (2001) Osmotic adjustment and root growth associated with drought preconditioning-enhanced heat tolerance in Kentucky bluegrass. Crop Sci 41:1168–1173Google Scholar
  18. Jiang Y, Wang K (2006) Growth, physiological and anatomical responses of creeping bentgrass cultivars to different depths of waterlogging. Crop Sci 46:2420–2426CrossRefGoogle Scholar
  19. Kennedy RA, Rumpho ME, Fox TC (1992) Anaerobic metabolism in plants. Plant Physiol 100:1–6PubMedCrossRefGoogle Scholar
  20. Kreuzwieser J, Furniss S, Rennenberg H (2002) Impact of waterlogging on the N-metabolism of flood tolerant and non-tolerant tree species. Plant Cell Environ 25:1039–1049CrossRefGoogle Scholar
  21. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685PubMedCrossRefGoogle Scholar
  22. Manchenko GP (1994) A handbook of detection of enzymes on electrophoretic gels. CRC Press, Boca Raton, p 42Google Scholar
  23. Pearson J, Havill DC (1988) The effect of hypoxia and sulphide on culture-grown wetland and non-wetland plants. II. Metabolic and physiological changes. J Exp Bot 39:432–439Google Scholar
  24. Perata P, Alpi A (1993) Plant responses to anaerobiosis. Plant Sci 93:1–17CrossRefGoogle Scholar
  25. Ratcliffe RG (1997) In vivo NMR studies of the metabolic responses of plant tissues to anoxia. Ann Bot (Lond) 79(Suppl A):39–48Google Scholar
  26. Razmjoo K, Kaneko S, Imada T (1993) Varietal differences of some cool-season turfgrass species in relation to heat and flood stress. Int Turf Res J 7:636–642Google Scholar
  27. Richard B, Aschi-Smiti S, Gharbi I, Brouquisse R (2006) Cellular and molecular mechanism of plant tolerance to waterlogging. In: Huang B (ed) Plant–environment interaction. CRC Press, Boca Raton, pp 177–208Google Scholar
  28. Roberts JKM, Chang K, Webster C, Callis J, Walbot V (1989) Dependence of ethanolic fermentation, cytoplasmic pH regulation, and viability on the activity of alcohol dehydrogenase in hypoxic maize root tips. Plant Physiol 89:1275–1278PubMedCrossRefGoogle Scholar
  29. Sachs MM, Freeling M, Okimoto R (1980) The anaerobic proteins of maize. Cell 20:761–767PubMedCrossRefGoogle Scholar
  30. SAS Institute Inc (2004) SAS procedures guide, release 9.1 edition. SAS, CaryGoogle Scholar
  31. Setter TL, Waters I (2003) Review of prospects for germplasm improvement for waterlogging tolerance in wheat, barley and oats. Plant Soil 253:1–34CrossRefGoogle Scholar
  32. Wang KH, Jiang YW (2007) Waterlogging tolerance of Kentucky bluegrass cultivars. HortScience 42:386–390Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Department of Crop and Soil Environmental SciencesVirginia Polytechnic Institute and State UniversityBlacksburgUSA
  2. 2.Department of AgronomyPurdue UniversityWest LafayetteUSA

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