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Plant and Soil

, Volume 311, Issue 1–2, pp 131–140 | Cite as

Effects of calcium on nickel tolerance and accumulation in Alyssum species and cabbage grown in nutrient solution

  • Rufus L. Chaney
  • Kuang-Yu Chen
  • Yin-Ming Li
  • J. Scott Angle
  • Alan J. M. Baker
Regular Article

Abstract

Nickel (Ni) phytoextraction using hyperaccumulator plant species to accumulate Ni from mineralized and contaminated soils rich in Ni is undergoing commercial development. Serpentinite derived soils have a very low ratio of Ca/Mg among soils due the nature of the parent rock. In crop plants, soil Ca reduces Ni uptake and phytotoxicity, so it is possible that the low Ca of serpentine soils could limit hyperaccumulator plant tolerance of serpentine soils used for commercial phytomining. In this study, we investigated the effects of varied Ca concentration in the presence of high Mg characteristic of serpentine soils on Ni uptake and tolerance by serpentine-endemic species Alyssum murale Waldst. et Kit. and A. pintodasilvae T.R. Dudley in comparison with cabbage (Brassica oleracea L. var. capita) in a nutrient solution study. The levels of Ca and Mg used were based on serpentine and normal soils, and Ni was based on achieving over 1% Ni in Alyssum shoots in preliminary tests. Varied solution concentrations of Ni (31.6–1,000 μM for Alyssum, 1.0–10 μM for cabbage) and Ca (0.128–5 mM) were used in a factorial experimental design; 2 mM Mg was used to mimic serpentine soils. Alyssum spp. showed much greater tolerance to high Ni, high Mg, and low Ca solution concentrations than cabbage. For Alyssum spp., Ni induced phytotoxicity was only apparent at 1,000 μM Ni with relatively low and high Ca/Mg quotient. In the 1,000 μM Ni treatment, shoot Ni concentrations ranged from 8.18 to 22.8 g kg−1 for A. murale and 7.60 to 16.0 g kg−1 for A. pintodasilvae. Normal solution Ca concentrations (0.8–2 mM) gave the best yield across all Ni treatments for the Alyssum species tested. It was clear that solution Ca levels affected shoot Ni concentration, shoot yield and Ni translocation from root to shoot, but the relation was non-linear, increasing with increasing Ca up to 2 mM Ca, then declining at the highest Ca. Our results indicate that Ca addition to high Mg serpentine soils with very low Ca/Mg ratio may reduce Ni phytotoxicity and improve annual Ni phytoextraction by Alyssum hyperaccumulator species. Removal of shoot biomass in phytomining will require Ca application to maintain full yield potential.

Keywords

Hyperaccumulation Magnesium Phytoextraction Phytoremediation serpentine 

Notes

Acknowledgments

We would like to thank C.E. Green for her assistance with ICP analysis and Mr. Alex Yellen cooperated in conducting a preliminary experiment to find the range of Ni tolerated by these species.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Rufus L. Chaney
    • 1
  • Kuang-Yu Chen
    • 2
  • Yin-Ming Li
    • 3
  • J. Scott Angle
    • 4
  • Alan J. M. Baker
    • 5
  1. 1.USDA-Agricultural Research Service, Environmental Management & Byproduct Utilization LaboratoryBeltsvilleUSA
  2. 2.Department of Plant Science and Landscape ArchitectureUniversity of MarylandCollege ParkUSA
  3. 3.Viridian Environmental LLCHoustonUSA
  4. 4.School of AgricultureUniversity of GeorgiaAthensUSA
  5. 5.School of BotanyThe University of MelbourneParkvilleAustralia

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