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Plant and Soil

, Volume 311, Issue 1–2, pp 121–129 | Cite as

Changes in lipid composition, water relations and gas exchange in leaves of two young ‘Chemlali’ and ‘Chetoui’ olive trees in response to water stress

  • Mokhtar Guerfel
  • Olfa Baccouri
  • Dalenda Boujnah
  • Mokhtar Zarrouk
Regular Article

Abstract

The comparative responses of two young olive trees (Olea europaea L. ‘Chemlali’ and ‘Chetoui’) to drought stress were investigated during 1 month. Three-month-old own-rooted plants were subjected to two irrigation treatments: WW (well watered plants that were irrigated with fresh water to maintain a soil water content close to field capacity), and WS (water stressed plants by withholding water). Leaf water potential, gas exchange and leaf lipid composition were studied. ‘Chemlali’ was able to maintain higher leaf CO2 assimilation rate and leaf stomatal conductance throughout the drought cycle compared to ‘Chetoui’. Water stress induced a larger decrease in the total lipid content in ‘Chetoui’ than in ‘Chemlali’. Interestingly, the constitution of different lipid classes was highly altered in ‘Chetoui’. Lipid changes in Chemlali, a drought tolerant cultivar, revealed more stability of its cellular membranes to drought stress as compared to the drought susceptible olive cultivar, Chétoui. Furthermore, in comparison to the controls, drought stressed plants showed an increase in the degree of unsaturation of leaf lipids in the two olive cultivars. Moreover, the results observed in Chemlali showed that besides changes in lipids composition this cultivar may have an efficient defence strategy which can be related on antioxidative production against oxidative stress.

Keywords

Drought stress Fatty acids Leaf lipids Olea europaea L. 

Abbreviations

DGDG

Digalactosyldiacylglycerol

DW

Dry weight

GL

Galactolipids

LOX

Lipoxygenase

MGDG

Monogalactosyldiacylglycerol

NL

Neutral lipids

PC

Phosphatidylcholine

PE

Phosphatidylethanolamine

PG

Phosphatidylglycerol

PI

Phosphatidylinositol

PL

Phospholipids

References

  1. Allakhverdiev SI, Kinoshita M, Inaba M, Suzuki I, Murata N (2001) Unsaturated fatty acids in membrane lipids protect the photosynthetic machinery against salt-induced damage in Synechococcus. Plant Physiol 125:1842–1853PubMedCrossRefGoogle Scholar
  2. Allen C, Good P (1971) Acyl lipid in photosynthetic systems. Methods Enzymol 23:523–547CrossRefGoogle Scholar
  3. Ben Ammar W, Nouairi I, Zarrouk M, Jemal F (2007) Cadmium stress induces changes in the lipid composition and biosynthesis in tomato (Lycopersicon esculentum Mill.) leaves. Plant Growth Regul 53:75–85CrossRefGoogle Scholar
  4. Ben Youssef N, Nouairi I, Ben Temime S, Taamalli W, Zarrouk M, Ghorbel MH et al (2005) Effets du cadmium sur le métabolisme des lipides de plantules de colza (Brassica napus L.). C R Biol 328:745–757PubMedCrossRefGoogle Scholar
  5. Bosabalidis AM, Kofidis G (2002) Comparative effects of drought stress on leaf anatomy of two olive cultivars. Plant Sci 163:375–379CrossRefGoogle Scholar
  6. Boujnah D (1997) Variations morphologiques anatomiques et écophysiologiques en rapport avec la résistance à la sécheresse chez l'olivier (Olea europaea L.). Ph.D Thesis. University of Gent, BelgiumGoogle Scholar
  7. Chartzoulakis K, Patakas A, Bosabalidis AM (1999) Changes in water relations, photosynthesis and leaf anatomy induced by intermittent drought in two olive cultivars. Environ Exp Bot 42:113–120CrossRefGoogle Scholar
  8. Chartzoulakis K, Loupasaki M, Bertaki M, Androulakis I (2002) Effects of NaCl salinity on growth, ion content and CO2 assimilation rate of six olive cultivars. Sci Hortic (Amsterdam) 96:235–247CrossRefGoogle Scholar
  9. Chen SY, Liu J (1991) The effect of water stress on membrane fluidity of leaf mitochodria of sugarcane and its relation to membrane lipid peroxidation. Acta Phytophysiol Sinica 17:285–289Google Scholar
  10. Cornic G (2000) Drought stress inhibits photosynthesis by decreasing stomatal aperture – not by affecting ATP synthesis. Trends Plant Sci 5:187–188CrossRefGoogle Scholar
  11. Dakhma WS, Zarrouk M, Chérif A (1995) Effects of drought-stress on lipids of rape leaves. Phytochemistry 40:1383CrossRefGoogle Scholar
  12. Douce R (1964) Identification et dosage de quelques glycérophosphatides dans des souches normales et tumorales de scosonères cultivés in vitro. C R Acad Sci Paris 259:3066–3068Google Scholar
  13. Fernandez JE, Diaz-Espejo A, Infante JM, Duran P, Palomo MJ, Chamorro V et al (2006) Water relations and gas exchange in olive trees under regulated deficit irrigation and partial rootzone drying. Plant Soil 284:273–291CrossRefGoogle Scholar
  14. Gigon A, Matos AR, Laffray D, Zuily-Fodil Y, Pham-Thi AT (2004) Effect of drought stress on lipid metabolism in the leaves of Arabidopsis thaliana (Ecotype Columbia). Ann Bot 94:345–351PubMedCrossRefGoogle Scholar
  15. Gimenez C, Fereres E, Ruz C, Orgaz F (1997) Water relations and gas exchange of olive trees: diurnal and seasonal patterns of leaf water potential, photosynthesis and stomatal conductance. Acta Hortic 449:411–415Google Scholar
  16. Gounaris K, Mannock DA, Sen A, Brain APR, Williams WP, Quinn PJ (1983) Polyunsaturated fatty acyl residues of galactolipids are involved in the control of bilayer/non-bilayer lipid transitions in higher plant chloroplasts. Biochim Biophys Acta 732:229–242CrossRefGoogle Scholar
  17. Graham D, Patterson BD (1982) Responses of plants to low, nonfreezing temperatures: proteins, metabolism, and acclimation. Annu Rev Plant Physiol 33:347–372CrossRefGoogle Scholar
  18. Gregoriou K (1999) Shading effect in photosynthesis of olive, cv. ‘‘Koroneiki’’, and the implications in shoot and fruit production. PhD Thesis, Agricultural University of Athens, GreeceGoogle Scholar
  19. Gruszecki WI, Strzalka K (1991) Does xanthophyll cycle take part in the regulation of fluidity of the thylakoid membrane? Biochim Biophys Acta 1060:310–314CrossRefGoogle Scholar
  20. Guerfel M, Baccouri B, Boujnah D, Zarrouk M (2007) Seasonal changes in water relations and gas exhange in leaves of two Tunisian olive (Olea europaea L.) cultivars under water deficit. J Hortic Sci Biotechnol 82:721–726Google Scholar
  21. Hamrouni I, Ben Salah H, Marzouk B (2001) Effects of water-deficit on lipids of safflower aerial parts. Phytochemistry 58:277–280PubMedCrossRefGoogle Scholar
  22. Harwood JL (1996) Recent advances in the biosynthesis of plant fatty acid. Biochim Biophys Acta 1301:7–56PubMedGoogle Scholar
  23. Harwood JL (1998) Environmental effects on plant lipid biochemistry. In: Harwood JL (ed) Plant lipid biosynthesis—fundamentals and agricultural applications. Cambridge University Press, Cambridge, pp 305–363Google Scholar
  24. Kodama H, Hamada T, Horiguchi G, Nishimura M, Iba K (1994) Genetic enhancement of cold tolerance by expression of a gene for chloroplast [omega]-3 fatty acid desaturase in transgenic tobacco. Plant Physiol 105:601–605PubMedGoogle Scholar
  25. Lawlor DW, Cornic G (2002) Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants. Plant Cell Environ 25:275–294PubMedCrossRefGoogle Scholar
  26. Lepage M (1967) Identification and composition of turp in root lipids. Lipids 2:244–250PubMedCrossRefGoogle Scholar
  27. Loreto F, Centritto M, Charztoulakis K (2003) Photosynthetic limitations in olive cultivars with different sensitivity to salt stress. Plant Cell Environ 26:595–601CrossRefGoogle Scholar
  28. Metcalfe D, Shmitz A, Pelka JR (1966) Rapid preparation of fatty acid esters from lipids for gas chromatographic analysis. Anal Chem 38:524–535CrossRefGoogle Scholar
  29. Mikami K, Murata N (2003) Membrane fluidity and the perception of environmental signals in cyanobacteria and plants. Prog Lipid Res 42:527–543PubMedCrossRefGoogle Scholar
  30. Monteiro de Paula F, Pham Thi AT, Vieira da Silva J, Justin AM, Demandre C, Mazliak P (1990) Effects of water stress on the molecular species composition of polar lipids from Vigna unguiculata L. leaves. Plant Sci 66:185–193CrossRefGoogle Scholar
  31. Moon BY, Higashi S-I, Gombos Z, Murata N (1995) Unsaturation of the membrane lipids of chloroplasts stabilizes the photosynthetic machinery against low-temperature photoinhibition in transgenic tobacco plants. Proc Natl Acad Sci USA 92:6219–6223PubMedCrossRefGoogle Scholar
  32. Murata N, Higashi SI, Fujimura Y (1990) Glycerolipids in various preparations of photosystem II from spinach chloroplasts. Biochim Biophys Acta 1019:261–268CrossRefGoogle Scholar
  33. Murphy DJ (1986) The molecular organization of the photosynthetic membranes of higher plants. Biochim Biophys Acta 864:33–94Google Scholar
  34. Nouairi I, Ben Ammar W, Ben Youssef N, Ben Miled Daoud D, Ghorbel MH, Zarrouk M (2006) Comparative study of cadmium effects on membrane lipid composition of Brassica juncea and Brassica napus leaves. Plant Sci 130:165–170Google Scholar
  35. Pham Thi AT, Vieira da Silva J, Mazliak P (1990) The role of membrane lipids in drought resistance of plants. Bull Soc Bot Fr 137:99–144Google Scholar
  36. Quartacci MF, Pinzino C, Sgherri CLM, Dalla Vecchia F, Navari-Izzo F (2000) Growth in excess copper induces changes in the lipid composition and fluidity of PSII-enriched membranes in wheat. Physiol Plant 108:87–93CrossRefGoogle Scholar
  37. Quinn PJ, Willliams WP (1983) The structural role of lipids in photosynthetic membranes. Biochim Biophys Acta 737:223–266Google Scholar
  38. Repellin A, Pham-Thi AT, Tashakorie A, Sahsah Y, Daniel C, Zuily-Fodil Y (1997) Leaf membrane lipids and drought tolerance in young coconut palms (Cocos nucifera L.). Eur J Agron 6:25–33CrossRefGoogle Scholar
  39. Routaboul JM, Fischer S, Browse J (2000) Trienoic fatty acids are required for photosynthesis at low temperatures. Plant Physiol 124:1697–1705PubMedCrossRefGoogle Scholar
  40. Sgherri CLM, Pinzino C, Navari-Izzo F (1996) Sunflower seedlings subjected to increasing stress by water deficit: changes in O2 ·-production related to the composition of thylakoid membranes. Physiol Plant 96:446–452CrossRefGoogle Scholar
  41. Smirnoff N (1993) The role of active oxygen in the response to water deficit and desiccation. New Phytol 125:27–58CrossRefGoogle Scholar
  42. Sofo A, Dichio B, Xiloyannis C, Masia A (2004) Lipoxygenase activity and proline accumulation in leaves and roots of olive trees in response to drought stress. Physiol Plant 121:58–65PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Mokhtar Guerfel
    • 1
  • Olfa Baccouri
    • 1
  • Dalenda Boujnah
    • 2
  • Mokhtar Zarrouk
    • 1
  1. 1.Laboratoire Caractérisation et Qualité de l’Huile d’OliveCentre de Biotechnologie de Borj CédriaHammam-LifTunisia
  2. 2.Institut de l’OlivierSousseTunisia

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