Plant and Soil

, Volume 304, Issue 1–2, pp 267–275 | Cite as

Comparison of the bacterial communities established on the mycorrhizae formed on Pinus thunbergii root tips by eight species of fungi

  • Ryota Kataoka
  • Takeshi Taniguchi
  • Hiroyuki Ooshima
  • Kazuyoshi Futai
Regular Article


The ectomycorrhizal (ECM) fungi associated with Pinus thunbergii seedlings grown on sand dune were identified by molecular method, and the diversity of bacteria associated with ECM and Extraradical mycelium were examined by Denaturing Gradient Gel Electrophoresis (DGGE) of PCR-amplified 16S rDNA. The mycorrhizal formation rate of 1-year old P. thunbergii seedlings was more than 95%. Cenococcum geophilum was the most dominant ECM fungus, followed by T01, RFLP-8, Russula spp., and Suillus sp. Bacterial community was most diverse with C. geophilum- and RFLP-8-mycorrhiza. Sequencing analysis showed that Burkholderia spp. and Bradyrhizobium spp. were on the surface of ECM short root of seven ECM. The fungi detected as extraradical mycelium using DGGE of 18S rDNA were Suillus bovinus and RFLP-8-mycorrhiza. Bacterial community on the extraradical mycelium was more diverse than those on ECM root tip. Burkholderia spp. and Bradyrhizobium spp. were found also on extraradical mycelium.


Bacteria Burkholderia Bradyrhizobium DGGE Ectomycorrhiza Mycorrhizosphere 



We thank Prof. Zaki A. Siddiqui, Aligarh Muslim University, for his correction in English and Yuko Takeuchi, Laboratory of Environmental Mycoscience, Kyoto University, for her critical suggestions.


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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Ryota Kataoka
    • 1
  • Takeshi Taniguchi
    • 1
  • Hiroyuki Ooshima
    • 2
  • Kazuyoshi Futai
    • 1
  1. 1.Laboratory of Environmental Mycoscience, Graduate School of AgricultureKyoto UniversityKyotoJapan
  2. 2.Department of Applied Biology and ChemistryTokyo University of AgricultureTokyoJapan

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