Comparison of the bacterial communities established on the mycorrhizae formed on Pinus thunbergii root tips by eight species of fungi
- 258 Downloads
The ectomycorrhizal (ECM) fungi associated with Pinus thunbergii seedlings grown on sand dune were identified by molecular method, and the diversity of bacteria associated with ECM and Extraradical mycelium were examined by Denaturing Gradient Gel Electrophoresis (DGGE) of PCR-amplified 16S rDNA. The mycorrhizal formation rate of 1-year old P. thunbergii seedlings was more than 95%. Cenococcum geophilum was the most dominant ECM fungus, followed by T01, RFLP-8, Russula spp., and Suillus sp. Bacterial community was most diverse with C. geophilum- and RFLP-8-mycorrhiza. Sequencing analysis showed that Burkholderia spp. and Bradyrhizobium spp. were on the surface of ECM short root of seven ECM. The fungi detected as extraradical mycelium using DGGE of 18S rDNA were Suillus bovinus and RFLP-8-mycorrhiza. Bacterial community on the extraradical mycelium was more diverse than those on ECM root tip. Burkholderia spp. and Bradyrhizobium spp. were found also on extraradical mycelium.
KeywordsBacteria Burkholderia Bradyrhizobium DGGE Ectomycorrhiza Mycorrhizosphere
We thank Prof. Zaki A. Siddiqui, Aligarh Muslim University, for his correction in English and Yuko Takeuchi, Laboratory of Environmental Mycoscience, Kyoto University, for her critical suggestions.
- Agerer R (1987–1997) In Colour atlas of Ectomycorrhizae: Einhorn-Verlag Eduard Dietenberger, Schwäbisch GmündGoogle Scholar
- Burke DJ, Kretzer AM, Rygiewicz PT, Topa MA (2006) Soil bacterial diversity in a loblolly pine plantation: influence of ectomycorrhizas and fertilization. FEMS Microbiol Ecol 53:409–419Google Scholar
- Colwell RK (2005) EstimateS: statistical estimation of species richness and shared species from samples. [http://viceroy.eeb.uconn.edu/EstimateS]
- Deacon JW, Fleming LV (1992) Interactions of ectomycorrhizal fungi. In: Allen MF (ed) Mycorrhiza functioning: an integrative plant–fungal process. Chapman and Hall, , NY, USA, pp 249–300Google Scholar
- Garbaye J, Duponnois R (1992) Specificity and function of mycorrhization helper bacteria (MHB) Associated with the Pseudotsuga menziesii–Laccaria laccata symbiosis. Symbiosis 14:335–244Google Scholar
- Madhukar BK, Keith NE, Hugues BM, Linda ET, Clapperton MJ (2002) Bacterial diversity associated with subalpine fir (Abies lasiocarpa) ectomycorrhizae following wildfire and salvage-logging in central British Columbia. Can J Microbiol 48:611–625Google Scholar
- Smith SE, Read DJ (1997) Mycorrhizal symbiosis, 2nd edn. Academic Press, New york, NY, USAGoogle Scholar
- Tadaki Y (1992) The ecological succession in coasts and coastal forests. In: Tadaki Y (ed) Japanese coastal forests: the many sided environmental functions and the applications (in Japanese). Soft Science, Tokyo, Japan, pp 52–57Google Scholar
- Trappe JM (1964) Mycorrhizal hosts and distribution of Cenococcum graniforme. Lloydia 27:100–106Google Scholar