Advertisement

Plant and Soil

, Volume 290, Issue 1–2, pp 61–68 | Cite as

Cadmium induces premature xylogenesis in barley roots

  • Katarína Ďurčeková
  • Jana Huttová
  • Igor Mistrík
  • Marta Ollé
  • Ladislav Tamás
Original Paper

Abstract

The effect of Cd on H2O2 production, peroxidase (POD) activity and root hair formation were analyzed in barley root. Cd causes a strong H2O2 burst in the root region 0–6 mm behind the root tip. POD activity was activated in root tip and raised toward the root base in Cd treated roots. In situ analyses showed that both elevated H2O2 production and POD activity are localized in the early metaxylem vascular bundles. Cd induces root hair formation in the region 2 to 4 mm behind the root tip that was not detected in control roots. These results suggest that Cd-induced root growth inhibition is at least partially the consequence of Cd-stimulated premature root development involving xylogenesis and root hair formation, which is correlated with shortening of root elongation zone and therefore with root growth reduction.

Keywords

Cadmium Hydrogen peroxide Lignification Peroxidase Root growth Root hair 

Abbreviations

AOS

Active oxygen species

CW

Cell wall

4-MN

4-Methoxy-1-naphthol

POD

Peroxidase

Notes

Acknowledgements

We wish to thank Margita Vašková for excellent technical assistance. This work was supported by the Grant agency VEGA, project No 2/4040/04.

References

  1. Barceló J, Vázquez MD, Poschenrieder C (1988a) Cadmium-induced structural and ultarstructural changes in the vascular systems of bush bean stems. Bot Acta 101:254–261Google Scholar
  2. Barceló J, Vázquez MD, Poschenrieder C (1988b) Structural and ultrastructural disorders in cadmium-treated bush bean plants (Phaseolus vulgaris L.). New Phytol 108:37–48CrossRefGoogle Scholar
  3. Benavides MP, Gallego SM, Tomaro ML (2005) Cadmium toxicity in plants. Braz J Plant Physiol 17:21–34CrossRefGoogle Scholar
  4. Bradford MN (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254PubMedCrossRefGoogle Scholar
  5. Böhm PAF, Zanardo FML, Ferrarese MLL, Ferrarese-Filho O (2006) Peroxidase activity and lignification in soybean growth-inhibition by juglone. Biol Plant 50:315–317CrossRefGoogle Scholar
  6. Chen YX, He YF, Luo YM, Yu YL, Lin Q, Wong MH (2003) Physiological mechanism of plant roots exposed to cadmium. Chemosphere 50:789–793PubMedCrossRefGoogle Scholar
  7. Cho U, Seo N (2005) Oxidative stress in Arabidopsis thaliana exposed to cadmium is due to hydrogen peroxide accumulation. Plant Sci 168:113–120CrossRefGoogle Scholar
  8. Christensen JH, Overney S, Rohde A, Diaz WA, Bauw G, Simon P, van Motagu M, Boerjan W (2001) The syringaldazine-oxidizing peroxidase PXP 3–4 from poplar xylem: cDNA isolation, characterization and expression. Plant Mol Biol 47:581–593PubMedCrossRefGoogle Scholar
  9. Cutler JM, Rians DW (1974) Characterisation of cadmium uptake by plant tissue. Plant Physiol 54:67–71PubMedCrossRefGoogle Scholar
  10. Ederli L, Reale L, Ferrari F, Pasqualini S (2004) Responses induced by high concentration of cadmium in Phragmites australis roots. Physiol Plant 121:66–74PubMedCrossRefGoogle Scholar
  11. Fan L, Linker R, Gepstein S, Tanimoto E, Yamamoto R, Neumann PM (2006) Progressive inhibition by water deficit of cell wall extensibility and growth along the elongation zone of maize roots is related to increased lignin metabolisms and progressive stelar accumulation of wall phenolics. Plant Physiol 140:603–612PubMedCrossRefGoogle Scholar
  12. Ferrer MA, Calderón AA, Muñoz R, Ros Barceló A (1990) 4-methoxy-α-naphthol as a specific substrate for kinetic, zymographic and cytochemical studies on plant peroxidase activities. Phytochem Anal 1:63–69Google Scholar
  13. Gadd GM, White C (1993) Microbial treatment of metal pollution – a working biotechnology. Trends Biotechnol 11:353–359PubMedCrossRefGoogle Scholar
  14. Holm KB, Andreasen PH, Eckloff RM G, Kristensen BK, Rasmussen SK (2003) Three differentially expressed basic peroxidases from wound-lignifying Asparagus officinalis. J Exp Bot 54:2275–2284PubMedCrossRefGoogle Scholar
  15. Hossian MA, Hossian AKM, Kihara T, Koyama H, Hara T (2005) Aluminum-induced lipid peroxidation and lignin deposition are associated with an increase in H2O2 generation in wheat seedlings. Soil Sci Plant Nutr 51:223–230CrossRefGoogle Scholar
  16. Iannelli MA, Pietrini F, Fiore L, Petrilli L, Massacci A (2002) Antioxidant response to cadmium in Phragmites australis plants. Plant Physiol Biochem 40:977–982CrossRefGoogle Scholar
  17. Kopittke PM, Menzies NW (2006) Effect of Cu toxicity on growth of cowpea (Vigna inguiculata). Plant Soil 279:287–296CrossRefGoogle Scholar
  18. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 227:680–685PubMedCrossRefGoogle Scholar
  19. Mäder M, Nessel A, Schloss P (1986) Cell compartmentation and specific roles of izoenzymes. In: Greppin H, Penel C, Gaspar T (eds) Molecular and physiological aspects of plant peroxidases. University Press, Geneva, Switzerland, pp 246–260Google Scholar
  20. McCarthy I, Romero-Puertas MC, Palma JM, Sandalio LM, Corpas FJ, Gómez M, Del Río LA (2001) Cadmium induces senescence symptoms in leaf peroxisomes of pea plants. Plant Cell Environ 24:1065–1073CrossRefGoogle Scholar
  21. Neil S, Desikan R, Hancock J (2002) Hydrogen peroxide signaling. Curr Opin Plant Biol 5:388–359CrossRefGoogle Scholar
  22. Olmos E, Martínez-Solano JR, Piqueras A, Hellín E (2003) Early steps in the oxidative burst induced by cadmium in cultured tobacco cells (BY-2 line). J Exp Bot 54:291–301PubMedCrossRefGoogle Scholar
  23. Olson PD, Varner JE (1993) Hydrogen peroxide and lignification. Plant J 4:887–892CrossRefGoogle Scholar
  24. Ostegaard L, Teilum K, Mirza O, Mattsson O, Petersen M, Welinder KG, Mundy J, Gajhede M, Henriksen A (2000) Arabidopsis ATP A2 peroxidase. Expression and high-resolution structure of plant peroxidase with implication for lignification. Plant Mol Biol 44:231–243CrossRefGoogle Scholar
  25. Pang A, Catesson A, Francesch C, Rolando C, Goldberg R (1989) On substrate specificity of peroxidases involved in the lignification process. J Plant Physiol 135:325–329Google Scholar
  26. Ranieri A, Castagna A, Scebba F, Careri M, Zagnoni I, Predieri G, Pagliari M, Sanita di Toppi L (2005) Oxidative stress and phytochelatin characterisation in bread wheat exposed to cadmium excess. Plant Physiol Biochem 43:45–54PubMedCrossRefGoogle Scholar
  27. Reisfeld RA, Levis UJ, Williams DE (1962) Disk electrophoresis of basic proteins and peptides on polyacrylamide gels. Nature 195:281–283PubMedCrossRefGoogle Scholar
  28. Rellán-Ávarez R, Ortega-Villasante C, Álvarez-Fernández A, del Campo FF, Hernández LE (2006) Stress responses of Zea mays to cadmium and mercury. Plant Soil 279:41–50CrossRefGoogle Scholar
  29. Ros Barceló A (1998) The generation of H2O2 in the xylem of Zinnia elegans is mediated by an NADPH-oxidase-like enzyme. Planta 207:207–216CrossRefGoogle Scholar
  30. Ros Barceló A, Aznar-Asensio GJ (2002) Basic peroxidases in cell walls of plants belonging to Asteraceae family. J Plant Physiol 159:339–345CrossRefGoogle Scholar
  31. Sandalio LM, Dalurzo HC, Gómez M, Romero-Puertas MC, del Río LA (2001) Cadmium-induced changes in the growth and oxidative metabolism of pea plants. J Exp Bot 52:2115–2126PubMedGoogle Scholar
  32. Sanita di Toppi L, Gabbrielli R (1999) Response to cadmium in higher plants. Environ Exp Bot 41:105–130CrossRefGoogle Scholar
  33. Schützendübel A, Schwanz P, Teichmann T, Gross K, Langenfeld-Heyser R, Godbold DL, Polle A (2001) Cadmium-induced changes in antioxidative systems, hydrogen peroxide content, and differentiation in Scots pine roots. Plant Physiol 127:887–898PubMedCrossRefGoogle Scholar
  34. Šimonovičová M, Huttová J, Mistrík I, Široká B, Tamás L (2004) Peroxidase mediated hydrogen peroxide production in barley roots grown under stress conditions. Plant Growth Regul 44:267–275CrossRefGoogle Scholar
  35. Singh N, Ma LQ, Srivastava M, Rathinasabapathi B (2006) Metabolic adaptations to arsenic-induced oxidative stress in Pteris vittata L and Pteris ensiformis L. Plant Sci 170:274–282CrossRefGoogle Scholar
  36. Tamás L, Budíková S, Šimonovičová M, Huttová J, Široká B, Mistrík I (2006a) Rapid and simple method for Al-toxicity analysis in emerging barley roots during germination. Biol Plant 50:87–93CrossRefGoogle Scholar
  37. Tamás L, Bočová B, Huttová J, Mistrík I, Ollé M (2006b) Cadmium-induced inhibition of apoplastic ascorbate oxidase in barley roots. Plant Growth Regul 48:41–49CrossRefGoogle Scholar
  38. Vázquez MD, Poschenrieder C, Barceló J (1992) Ultrastructural effects and localization of low cadmium concentrations in bean roots. New Phytol 120:215–226CrossRefGoogle Scholar
  39. Vitória AP, Rodriguez APM, Cunha M, Lea PJ, Azevedo RA (2003) Structural changes in radish seedlings exposed to cadmium. Biol Plant 47:561–568CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Katarína Ďurčeková
    • 1
  • Jana Huttová
    • 1
  • Igor Mistrík
    • 1
  • Marta Ollé
    • 1
  • Ladislav Tamás
    • 1
  1. 1.Institute of BotanySlovak Academy of SciencesBratislavaSlovak Republic

Personalised recommendations