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Cadmium induces premature xylogenesis in barley roots

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Abstract

The effect of Cd on H2O2 production, peroxidase (POD) activity and root hair formation were analyzed in barley root. Cd causes a strong H2O2 burst in the root region 0–6 mm behind the root tip. POD activity was activated in root tip and raised toward the root base in Cd treated roots. In situ analyses showed that both elevated H2O2 production and POD activity are localized in the early metaxylem vascular bundles. Cd induces root hair formation in the region 2 to 4 mm behind the root tip that was not detected in control roots. These results suggest that Cd-induced root growth inhibition is at least partially the consequence of Cd-stimulated premature root development involving xylogenesis and root hair formation, which is correlated with shortening of root elongation zone and therefore with root growth reduction.

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Abbreviations

AOS:

Active oxygen species

CW:

Cell wall

4-MN:

4-Methoxy-1-naphthol

POD:

Peroxidase

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Acknowledgements

We wish to thank Margita Vašková for excellent technical assistance. This work was supported by the Grant agency VEGA, project No 2/4040/04.

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Authors and Affiliations

  1. Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 14, SK-84523, Bratislava, Slovak Republic

    Katarína Ďurčeková, Jana Huttová, Igor Mistrík, Marta Ollé & Ladislav Tamás

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  1. Katarína Ďurčeková
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  2. Jana Huttová
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  3. Igor Mistrík
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  4. Marta Ollé
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  5. Ladislav Tamás
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Correspondence to Ladislav Tamás.

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Ďurčeková, K., Huttová, J., Mistrík, I. et al. Cadmium induces premature xylogenesis in barley roots. Plant Soil 290, 61–68 (2007). https://doi.org/10.1007/s11104-006-9111-6

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  • DOI: https://doi.org/10.1007/s11104-006-9111-6

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