Plant and Soil

, Volume 285, Issue 1–2, pp 369–379 | Cite as

Elevated CO2 alleviates the effects of low P on the growth of N2-fixing Acacia auriculiformis and Acacia mangium

  • Nguyen Tran Nguyen
  • Pravat Kumar Mohapatra
  • Kounosuke Fujita
Original Paper


Nodulated seedlings of Acacia auriculiformis Cunn. ex Benth and Acacia mangium Willd were grown with different phosphorus (P) regimes for 90 days, and half of them were exposed to elevated CO2 (800 μl l−1) during the last 30 days. Under ambient CO2, plant growth and the amount of N fixed symbiotically in N2-fixing seedlings decreased with the decrease of supplied P; this relationship did not occur under elevated CO2. The increase in plant biomass by elevated CO2 at low P was accompanied by the increase in internal P use efficiency, the amount of N fixed symbiotically and N use efficiency. Elevated CO2 recovered the low P-induced reduction in leaf dry matter per unit area or unit fresh weight, but it had no effect on the low P-induced increase in partitioning dry matter to roots. These results suggest that elevated CO2 alleviates the low P effect mainly by increasing the use efficiency of internal P for plant growth and symbiotic N2 fixation, and the source-sink relationship is possibly an important driving force for this effect of elevated CO2 in A. auriculiformis and A. mangium.


Elevated CO2 Low P tolerance P use efficiency Source-sink relationship Symbiotic N2 fixation 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Almeida JPF, Luscher A, Frehner M, Oberson A, Nosberger J (1999) Partitioning of P and the activity of root acid phosphatase in white clover (Trifolium repens L.) are modified by increased atmospheric CO2 and P fertilization. Plant Soil 210:159–166CrossRefGoogle Scholar
  2. Almeida JPF, Hartwig UA, Frehner M, Noshgerger J, Luscher A (2000) Evidence that P deficiency induces N feedback regulation of symbiotic N2 fixation in white clover (Trifolium repens L.). J Exp Bot 51:1289–1297PubMedCrossRefGoogle Scholar
  3. Amthor JS (1991) Respiration in a future, higher-CO2 world. Plant Cell Environ 14:13–20CrossRefGoogle Scholar
  4. Arnone JA III, Gordon JC (1990) Effect of nodulation, nitrogen fixation and CO2 enrichment on the physiology, growth and dry mass allocation of seedlings of Alnus rubra Bong. New Phytol 116:55–66CrossRefGoogle Scholar
  5. Bates TR, Lynch JP (1996) Stimulation of root hair elongation in Arabidopsis thaliana by low phosphorus availability. Plant Cell Environ 19:529–438CrossRefGoogle Scholar
  6. Cadisch G, Sylvester_Bradley R, Boller BC, Nosberger J (1993) Effect of phosphorus and potassium on N2 fixation (15N-dilution) of field-grown Centrosema acutifolium and C. macrocarpum. Field Crop Res 31:329–340CrossRefGoogle Scholar
  7. Cakmak I, Hengeler C, Marschner H (1994) Partitioning of shoot and root dry matter and carbohydrates in bean plants suffering from phosphorus, potassium and magnesium deficiency. J Exp Bot 45:1245–1250Google Scholar
  8. Clarkson DT (1985) Factors affecting mineral nutrient acquisition by plants. Ann Rev Plant Physio 36:77–115Google Scholar
  9. Drake BG, Gonzalez-Meler MA, Long SP (1997) More efficient plants: a consequence of rising atmospheric CO2? Annu Rev Plant Phys 48:609–639CrossRefGoogle Scholar
  10. Evans JR, Schortemeyer M, McFarlane N, Atkin OK (2000) Photosynthetic characteristics of 10 Acacia species grown under ambient and elevated atmospheric CO2. Aust J Plant Physiol 27:13–25Google Scholar
  11. Fischer BU, Frehner M, Hebeisen T, Zanetti S, Stadelmann F, Luscher A, Hartwig UA, Hendrey GR, Blum H, Nosberger J (1997) Source-sink relations in Lolium perenne L. as reflected by carbohydrate concentrations in leaves and pseudo-stems during regrowth in a free air carbon dioxide enrichment (FACE) experiment. Plant Cell Environ 20:945–952CrossRefGoogle Scholar
  12. Fohse D, Claassen N, Jungk A (1988) Phosphorous efficiency of plants. I: External and internal P requirement and P uptake efficiency of different plant species. Plant Soil 110:101–109CrossRefGoogle Scholar
  13. Foyer C, Spencer C (1986) The relationship between phosphate status and photosynthesis in leaves. Effects on intracellular orthophosphate distribution, photosynthesis and assimilate partitioning. Planta 167:379–375CrossRefGoogle Scholar
  14. Fujita K, Kai Y, Takayanagi M, El-Shenny H, Adu-Gyanfi JJ, Mohapatra PK (2004) Genotypic variability of pigeonpea in distribution of photosynthetic carbon at low phosphorus level. Plant Sci 166:641–649CrossRefGoogle Scholar
  15. Gourley CJP, Allan DL, Russelle MP (1993) Defining phosphorus efficiency in plants. Plant Soil 155/156:282–292CrossRefGoogle Scholar
  16. Hartwig UA (1998) The regulation of symbiotic N2 fixation: a conceptual model of N feedback from the ecosystem to the gene expression level. Perspect Plant Ecol 1:92–120CrossRefGoogle Scholar
  17. Heim I, Hartwig UA, Nosberger J (1993) Current nitrogen fixation is involved in the regulation of nitrogenase activity in white cloves (Trifolium repens L.). Plant Physiol 103:1009–1014PubMedGoogle Scholar
  18. Israel DW (1987) Investigation of the role of phosphorus in symbiotic dinitrogen fixation. Plant Physiol 81: 835–840Google Scholar
  19. Israel DW, Rufty TWJ (1988) Influence of phosphorous nutrition on phosphorous and nitrogen utilization efficiencies and associated physiological responses in soybean. Crop Sci 28:954–960CrossRefGoogle Scholar
  20. Mimura T, Dietz KJ, Kaiser W, Schramm MJ, Kaiser G, Heber U (1990) Phosphate transport across biomembranes and cytosolic phosphate homeostasis in barley leaves. Planta 180:139–146CrossRefGoogle Scholar
  21. Mollier A, Pellerin S (1999) Maize root system growth and development as influenced by phosphorous deficiency. J Exp Bot 50:487–497CrossRefGoogle Scholar
  22. Niinemets U, Tenhunen JD, Canta NR, Chaves MM, Raria T, Pereira JS, Reynolds JF (1999) Interactive effects of nitrogen and phosphorus on the acclimation potential of foliage photosynthetic properties of cork oak, Quercus suber, to elevated atmospheric CO2 concentration. Glob Change Biol 5:455–470CrossRefGoogle Scholar
  23. Oberbauer SF, Stain BR, Tetcher N (1985) Effect of CO2 enrichment on seedling physiology and growth of two tropical tree species. Physiol Plantarum 65:352–356CrossRefGoogle Scholar
  24. Olivera M, Tejera N, Iribarne C, Acana A, Lluch C (2004) Growth, nitrogen fixation and ammonium assimilation in common bean (Phaseolus vulgaris): effect of phosphorus. Physiol Plantarum 121:498–505CrossRefGoogle Scholar
  25. Parsons R, Stanforth A, Raven JA, Sprent JI (1993) Nodule growth and activity may be regulated by a feedback mechanism involving phloem nitrogen. Plant Cell Environ 16:125–136CrossRefGoogle Scholar
  26. Poorter H, Gifford RM, Kriedemann PE, Wong SC (1992) A quantitative analysis of dark respiration and carbon content as factors in the growth response of plants to elevated CO2. Aust J Bot 40:501–513CrossRefGoogle Scholar
  27. Poorter H (1993) Interspecific variation in the growth response of plants to an elevated ambient CO2 concentration. Vegetatio 104/105:77–97CrossRefGoogle Scholar
  28. Poorter H, Van Berkel Y, Baxter R, Den Hertog J, Dijkstra P, Gifford RM, Griffin KL, Roumet C, Roy J, Wong SC (1997) The effect of elevated CO2 on the chemical composition and construction costs of leaves of 27 C3 species. Plant Cell Environ 20:472–482CrossRefGoogle Scholar
  29. Radin JW, Eidenbock MP (1984) Hydraulic conductance as a factor limiting leaf expansion of phosphorus deficient cotton plants. Plant Physiol 75:372–377PubMedGoogle Scholar
  30. Rao M, Terry N (1989) Leaf phosphate status, photosynthesis and carbon partitioning in sugar beet. I. Changes in growth, gas exchange and Calvin Cycle enzymes. Plant Physiol 90:814–819PubMedCrossRefGoogle Scholar
  31. Rao IM, Fredeen AL, Terry N (1993) Influence of phosphorus limitation on photosynthesis, carbon allocation and partitioning in sugar beet and soybean grown with a short photoperiod. Plant Physiol Biochem 31:223–231Google Scholar
  32. Reekie EG, Bazzza FA (1989) Competition and patterns of resource use among seedling of five tropical tress grown at ambient and elevated CO2. Oecologia 79:212–222CrossRefGoogle Scholar
  33. Ribet J, Drevon JJ (1996) The phosphorus requirement of N2-fixing and urea-fed Acacia mangium. New Phytol 132:383–390CrossRefGoogle Scholar
  34. Schortemeyer M, Atkin OK, McFarlane, Evans JR (1999) The impact of elevated atmospheric CO2 and nitrate supply on growth biomass allocation, nitrogen partitioning and N2 fixation of Acacia melanoxylon. Aust J Plant Physiol 26:737–747CrossRefGoogle Scholar
  35. Schortemeyer M, Atkin OK, McFarlane N, Evans JR (2002) N2 fixation by Acacia species increases under elevated atmospheric CO2. Plant Cell Environ 25:567–579CrossRefGoogle Scholar
  36. Sprent JI (1999) Nitrogen fixation and growth of non-crop species in diverse environments. Perspect Plant Ecol 2:149–162CrossRefGoogle Scholar
  37. Sprent JI, Parsons R (2000) Nitrogen fixation in legume and non-legume trees. Field Crop Res 65:183–196CrossRefGoogle Scholar
  38. Sun JS, Sands R, Simpson (1992a) Genotypic variation in growth and nodulation by seedlings of Acacia species. Forest Ecol Manag 55:209–223CrossRefGoogle Scholar
  39. Sun JS, Simpson RJ, Sands R (1992b) Nitrogenase activity of two genotypes of Acacia mangium as affected by phosphorus nutrition. Plant Soil 144:51–58CrossRefGoogle Scholar
  40. Sun JS, Simpson RJ, Sands R (1992c) Nitrogenase activity and associated budgets in seedlings of Acacia mangium measured with a flow-through system of the acetylene reduction assay, Aust. J Plant Physiol 19:97–107Google Scholar
  41. Turnbull JW, Midgley SJ, Cossalter C (1998) Tropical Acacia planted in Asia: an overview. In: Turnbull JW, Crompton HR, Pinyopusarerk K (eds) Recent developments in Acacia planting, Proceeding of an International Woekshop held in Hanoi, Vietnam, 17–30 October 1997. ACIAR Proceeding No. 82, pp 1–28Google Scholar
  42. Thomas RB, Richter DD, Ye H, Heine PR, Strain BR (1991) Nitrogen dynamics and growth of seedlings of and N-fixing tree (Gliricidia sepium (Jacp.) Walp.) exposed to elevated atmospheric carbon dioxide. Oecologia 88:415–421CrossRefGoogle Scholar
  43. Vadez V, Lim G, Durand P, Diem HG (1995) Comparative growth and symbiotic performance of four Acacia mangium provenances from Papua New Guinea in response to the supply of phosphorus at various concentrations. Biol Fert Soils 19:60–64CrossRefGoogle Scholar
  44. Vadez V, Lasso JH, Beck DP, Drevon JJ (1999) Variability of N2-fixation in common bean (Phaseolus vulgaris L.) under P deficiency is related to P use efficiency. N2-fixation tolerance to P deficiency. Euphytica 106:231–242CrossRefGoogle Scholar
  45. Vadez V, Drevon JJ (2001) Genotypic variability in phosphorus use efficiency for symbiotic N2 fixation in common bean (Phaseolus vulgaris). Agronomie 21:691–699CrossRefGoogle Scholar
  46. Williams ER, Matheson AC (1994) Experimental design and analysis for use in tree improvement. CSIRO-ACIAR, Australia, 174 ppGoogle Scholar
  47. Ziska LH, Hogan KP, Smith AP, Drake BG (1991) Growth and photosynthetic response of nine tropical species with long-term exposure to elevated carbon dioxide. Oecologia 86:383–389CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Nguyen Tran Nguyen
    • 1
  • Pravat Kumar Mohapatra
    • 2
  • Kounosuke Fujita
    • 1
  1. 1.Graduate School of Biosphere SciencesHiroshima UniversityHiroshimaJapan
  2. 2.School of Life ScienceSambalpur UniversityJyoti Vihar, SambalpurIndia

Personalised recommendations