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Plant and Soil

, Volume 276, Issue 1–2, pp 133–141 | Cite as

Effects of Aluminum on the Growth of Tea Plant and Activation of Antioxidant System

  • Faezeh Ghanati
  • Akio Morita
  • Hiromi Yokota
Article

Abstract

A possible connection between the effects of aluminum (Al) on the growth of tea plants and the active oxygen species scavenging system in root tips of intact tea plants and suspension-cultured tea cells was examined. Intact tea plants were treated with or without Al in a modified Hoagland solution, while suspension-cultured tea cells were treated with or without Al in a simple salt solution containing 3% sucrose and 0.2 mM calcium. Compared with the control treatments without Al, the activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) increased by Al both in roots of intact plants and cultured cells. The level of peroxidation of membrane lipids, as well as the activity of wall-bound peroxidas, the content of lignin and wall-bound phenols, however, reduced by the treatment with Al either in tea roots or in cultured tea cells. The results indicated that Al-induced increase in the activities of antioxidant enzymes, resulting in increased membrane integrity and delayed lignification and aging, can be considered as a possible reason for the stimulatory effects of Al on the growth of the tea plants and this is irrespect of the presence of other micronutrients and their interaction with Al.

Key words

aluminum tolerance antioxidant system Camellia sinensis lignin phenylalanine ammonia-lyase (PAL) reactive oxygen species (ROS) 

Abbreviations

APX

ascorbate peroxidase

CAT

catalase

CPO

covalently bound peroxidase

IPO

ionically bound peroxidase

MDA

malondialdehyde

PAL

phenylalanine ammonia-lyase

PO

peroxidase

ROS

reactive oxygen species

SOD

superoxide dismutase

SPO

soluble peroxidase

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References

  1. Alvarez, M E, Lamb, C 1997Oxidative burst mediated defense responses in plant disease resistanceScandalios, J G. eds. Oxidative Stress and the Molecular Biology of Antioxidant DefensesCold Spring Harbor Laboratory PressNew York815839Google Scholar
  2. Bradford, M M 1976A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnal. Biochem.72248254CrossRefPubMedGoogle Scholar
  3. Cakmak, I, Horst, W J 1991Effect of aluminum on lipid peroxidation, superoxide dismutase, catalase, and peroxidase activities in root tips of soybean (Glycine max)Physiol. Plant.83463468CrossRefGoogle Scholar
  4. Dat, J F, Montagu, M, Inze, D, Breusegem, F 2001Catalase-deficient tobacco plants: tools for in planta studies on the role of hydrogen peroxideRedox Report63742CrossRefPubMedGoogle Scholar
  5. Dual action of the active oxygen species during plant stress responses. CMLS. 57, 779–795.Google Scholar
  6. Vos, C H R, Schat, H, Waal, M A D., Vooijs, R, Ernst, W H O 1991Increased resistance to copper-induced damage of the root plasma membrane in copper tolerant Silene cucubalusPhysiol. Plant.82523528Google Scholar
  7. Ezaki, B, Katsuhara, M, Kawamura, M, Matsumoto, H 2001Different mechanisms of four aluminum (Al)-resistant transgenes for Al toxicity in ArabidopsisPlant Physiol.127918927CrossRefPubMedGoogle Scholar
  8. Fry, S C 1986Cross-linking of matrix polymers in the growing cell walls of angiospermsAnn. Rev. Plant Physiol.37 165186Google Scholar
  9. Fukuda, H, Komamine, A 1982Lignin synthesis and its related enzymes as markers of tracheary-element differentiation in single cells isolated from the mesophyll of Zinnia elegansPlanta155423430CrossRefGoogle Scholar
  10. Giannopolitis, C N, Ries, S K 1977Superoxide dismutase I Occurrence in higher plantsPlant Physiol.59309314Google Scholar
  11. Goldberg, R, Catesson, A M, Czaninski, Y 1983Some properties of syringaldazine oxidase, a peroxidase specifically involved in lignification processesZ. Pflanzenphysiol.110256277Google Scholar
  12. Iiyama, K, Wallis, A F A 1990Determination of lignin in herbaceous plants by an improved acetyl bromide procedureJ. Sci. Food Agric.51145161Google Scholar
  13. Kinraide, T B 1993Aluminum enhancement of plant growth in acid rooting media. A case of reciprocal alleviation of toxicity by two toxic cationsPlant Physiol.88619625Google Scholar
  14. Konishi, S 1992Promotive effects of aluminum on tea plant growthJARQ.262633Google Scholar
  15. Konishi, S, Miyamoto, S, Taki, T 1985Stimulatory effects of aluminum on tea plants growth under low and high phosphorus supplySoil Sci. Plant Nutr.31361368Google Scholar
  16. Kuboi, T, Kaji, M 1994Establishment of tea cell lines with high growth rateTea Res.8018 (in Japanese)Google Scholar
  17. Marschner, H 1995Mineral Nutrition of Higher PlantsAcademic PressLondonGoogle Scholar
  18. Matsumoto, H, Hirasawa, E, Morimura, S, Takahashi, E 1976Localization of aluminum in tea leavesPlant Cell Physiol.17627631Google Scholar
  19. Michiels, C, Raes, M, Toussaint, O, Remacle, J 1994Importance of Se-glutathione peroxidase, catalase, and Cu/Zn-SOD for cell survival against oxidative stressFree Radical Biol. Med.17235248CrossRefGoogle Scholar
  20. Morita, S, Kaminaka, H, Matsumura, T, Tanaka, K 1999Induction of rice cytosolic ascorbate peroxidase mRNA by oxidative stress; the involvement of hydrogen peroxide in oxidative stress signalingPlant CellPhysiol. 40417422Google Scholar
  21. Nakano, Y, Asada, K 1981Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplastsPlant Cell Physiol.22867880Google Scholar
  22. Ogawa, T, Matsumoto, C, Takenaka, C, Tezuka, T 2000Effects of Ca on Al-induced activation of antioxidant enzymes in the needles of Hinoki Cypress (Chamaecyparis obtusa)J. Forest Res.58185Google Scholar
  23. Pandolfini, T, Gabbrielli, R, Comparini, C 1992Nickel toxicity and peroxidase activity in seedlings of Triticum aestivum LPlant Cell Environ.15719275Google Scholar
  24. Schopfer, P 1996Hydrogen peroxide mediated cell wall stiffening in vitro in maize coleoptilesPlanta1994349CrossRefGoogle Scholar
  25. Sreenivasulu, N, Grimm, B, Wobus, U, Weschke, W 2000Differential response of antioxidant compounds to salinity stress in salt-tolerant and salt-sensitive seedlings of foxtail millet (Setaria italica)Physiol. Plant.109435442CrossRefGoogle Scholar
  26. Wakabayashi, K, Takayuki, H, Kamisaka, S 1997Osmotic stress suppresses cell wall stiffening and the increase in cell wall-bound ferulic and diferulic acids in wheat coleoptilesPlant Physiol.113967973PubMedGoogle Scholar
  27. Yamamoto, Y, Rikiishi, S, Chang, Y, Ono, K, Kasai, M, Matsumoto, H 1994Quantitative estimation of aluminum toxicity in cultured tobacco cells: Correlation between aluminum uptake and growth inhibitionPlant Cell Physiol.35575583Google Scholar
  28. Yamamoto, Y, Kobayashi, Y, Devi, R S, Rikiishi, S, Matsumoto, H 2002Aluminum toxicity is associated with mitochondrial dysfunction and production of reactive oxygen species in plant cellsPlant Physiol.1286372PubMedGoogle Scholar
  29. Zheng, X, Huystee, R. B. 1992Anionic peroxidase catalyzed ascorbic acid and IAA oxidation in the presence of hydrogen peroxide: A defense system against peroxidative stress in peanut plantPhytochemistry3118951898CrossRefGoogle Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  1. 1.Faculty of Science, Department of Plant ScienceTarbiat Modarres UniversityTehranIran
  2. 2.Faculty of Agriculture, Department of Applied Biological ChemistryShizuoka UniversityShizuokaJapan

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