Plant and Soil

, Volume 280, Issue 1–2, pp 115–126 | Cite as

Atrazine Tolerance of Grass Species with Potential for Use in Vegetated Filters in Australia

  • V. H. Popov
  • P. S. Cornish


The response of introduced (Pennisetum clandestinum Hochst.) and native (Stipa aristiglumis F. Muell., Themeda australis (R. Br.) Stapf, Danthonia spp. (L.) grasses to the herbicide atrazine was studied in plants with the potential for use in vegetated filters (biofilters) designed to reduce chemical loads in agricultural runoff. The response was detected by photosynthetic inhibition using leaf chlorophyll fluorescence. With continuous short-term (14 days) dosing with atrazine in sand culture, P. clandestinum showed the greatest tolerance, regardless of the dose (20–500 μg/L). In a clay vertosol soil in the glasshouse, the four species were tolerant to longer-term (84 days) application of three successive doses of simulated run-on, each dose containing 100 μg/L atrazine, a concentration which is comparable to the highest reported in runoff from agricultural land in Australia. Even with a subsequent single atrazine dose at ∼ ∼5000 μg/L, established plants of the four species showed signs of quick recovery (7–21 days) to normal photosynthetic activity. In a field experiment with simulated run-on, applied to P. clandestinum pasture in sandy loam soil, repeated doses at concentrations up to 1000 μg/L gave no significant response; only a subsequent single dose of 5000 μg/L had significant effects, from which plants soon recovered. Although damage from atrazine can be demonstrated with continuous dosing in non-adsorbing media, in soil culture the tolerance of the four selected species to repeat doses of atrazine shows they may be used confidently for biofiltering purposes. P. clandestinum was especially tolerant.


atrazine concentration buffer strips grass response Pennisetum clandestinum 


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  1. Aiken J T 2004 Redeveloping soil mapping key descriptors from generic soil series profiles for University of Western Sydney Hawkesbury campus soils. ISBN 1 920842 26 8 SuperSoil 2004 Published by The Regional Institute LtdGoogle Scholar
  2. Baconguis S E, Pameron F P and Navarro R V 1982 Grassland overgrazing degrades environment and rural life. CANOPY Int. 8Google Scholar
  3. Baker J L, Mickelson S K, Arora K and Misra A K 2000 The potential of vegetated filter strips to reduce pesticide transport. American Chemical Society Symposium Series, 2000Google Scholar
  4. Beadle, N C W 1981The Vegetation of AustraliaPress Syndicate of the University of CambridgeCambridgeGoogle Scholar
  5. Bjorkman, O, Demming, B 1987Photon Yield of O2 evolution and chlorophyll fluorescence characteristics at 77K among vascular plants of diverse originPlanta170489504Google Scholar
  6. Boey, A, Cooper, B 1996Central and north-west regions water quality program – Atrazine and its ecological significanceInternal Report, Department of Land and Water ConservationAustraliaGoogle Scholar
  7. Butler, W L, Kitajima, M 1975A tripartite model for chloroplast fluorescenceAvron, M eds. Proceedings of the 3-rd International Congress on PhotosynthesisElsevierAmsterdam1324Google Scholar
  8. Cole, I, Koen, T, Metcalfe, J, Johnston, W, Mitchell, M 2003Tolerance of Austrodanthonia fulva, Microlaena stipoides and Elymus scaber seedlings to nine herbicidesPlant Prot. Quart.181822Google Scholar
  9. Colwell, J D 1963The estimation of the phosphorus fertilizer requirements of wheat in southern New South WalesAust. J. Exp. Agr. Anim. Husb.3190197Google Scholar
  10. Corbett, J R 1974The Biochemical Mode of Action of PesticidesAcademic PressLondon. New YorkGoogle Scholar
  11. Cornish P S, Sun H and Popov V H 2004 An integrated landscape approach to biofiltration of agricultural pollutants. In Proceedings of American Water Resources Association (AWRA) Summer Specialty Conference on Riparian Ecosystems and Buffers: Multi-Scale Structure, Function, and Management, Olympic Valley CA, USAGoogle Scholar
  12. Gottesburen, B, Beulke, S, Heiermann, M, Pestemer, W 1994Field and laboratory experiments to validate predictory models of the expert system HERBASYS applied to problems of crops following the use of herbicidesWeed Res.346378Google Scholar
  13. Gronwald, J W 1994Resistance to photosystem II inhibiting herbicidesPowles, S BHoltum, J A M eds. Herbicide Resistance in Plants: Biology and BiochemistryLewisBoca, FL, USA2660Google Scholar
  14. Dosskey, M 2001Toward quantifying water pollution abatement in response to installing buffers on crop landEnviron. Manage.28577598CrossRefPubMedGoogle Scholar
  15. Dosskey, M 2002Setting priorities for research on pollution reduction functions of agricultural buffersEnviron. Manage.30641650CrossRefPubMedGoogle Scholar
  16. Dumas, J B 1981Sur les procédés de l’analyse organiqueAnnal. de ChimieXLVII195213Google Scholar
  17. Ferris, I G, Frecker, T C, Haigh, B M, Durrant, S 1992Herbicide adviser: a decision support system to optimise atrazine and chlorsulfuron activity and crop safetyComput. Electron. Agr.6295317Google Scholar
  18. Hansatech Instruments Ltd. 1986 An introduction to fluorescence measurements with the Plant Efficiency Analyser (PEA). Narborough rd., Pentney, King’s Lynn, Norfolk PE32 1JL, EnglandGoogle Scholar
  19. Houot, S, Topp, E, Yassir, A, Soulas, G 2000Dependence of accelerated degradation of atrazine on soil pH in French and Canadian soilsSoil Biol. Biochem.32615625CrossRefGoogle Scholar
  20. Jettner, R J, Walker, S R, Churchett, J D, Blamey, F P C, Adkins, S W, Bell, K 1999Plant sensitivity to atrazine and chlorsulfuron residues in a soil-free systemWeed Res.39287295CrossRefGoogle Scholar
  21. Jones C E 2001 The Great Salinity Debate: Part III. Soil organic matter: past lessons for future learning. Stipa Newsletter No: 15, 4–9; Reprinted in Australian Farm Journal, March 2001, 60–63Google Scholar
  22. Karssies L E and Prosser I P 1999 Guidelines for riparian filter strips for Queensland irrigators.CSIRO Land and Water Technical Report, 32/99Google Scholar
  23. Kautsky, H, Hirsh, A 1931Neue versuche zur kohlenstoffassimilationNaturwissenschaften19964Google Scholar
  24. Lerch, R N, Thurman, E M, Blanchard, P E 1999Hydroxyatrazine in soils and sedimentsEnviron. Toxicol. Chem.1821612168CrossRefGoogle Scholar
  25. Lodge, G M, McMillan, M G, Schipp, A J, Cook, A S 1994Effects of herbicides on wallaby grass (Danthonia spp.) 1. EstablishmentAust. J. Exp. Agr.34753757Google Scholar
  26. Lovett S and Price P 2001 Managing riparian lands in the sugar industry: a guide to principles and practices. Sugar Research & Development Corporation/Land & Water Australia, Brisbane. ISBN 0-9579313-0-1Google Scholar
  27. Mawhinney W A 1998 Liverpool Plains water quality project – 1996/98 – land use, pesticides use and their impact on water quality in the Liverpool Plains – DLWC, December 1998, ISSN 1329–8984Google Scholar
  28. McDougall K L 1989 The re-establishment of Themeda triandra (Kangaroo Grass): Implications for the restoration of grassland. Arthur Rylah Institute for Environmental Research – Technical Report Series No. 89. May 1989Google Scholar
  29. Moss S R 1995 Techniques for determination of herbicide resistance. In Proceedings of Brighton Crop Protection Conference – Weeds, UKGoogle Scholar
  30. National Registration Authority for Agricultural and Veterinary Chemicals (NRA) 1997 Technical Report on the NRA Review of Atrazine November 1997, NRA, Canberra. Available Online: Scholar
  31. NHMRC/ARMCANZ1996 Australian Drinking Water Quality GuidelinesNational Health and Medical Research Council and Agriculture and Resource Management Council of Australia and New Zealand. Resource Planning and Development CommissionAustraliaGoogle Scholar
  32. NRA2002Final Review Report on Atrazine. National Registration Authority for Agricultural and Veterinary Chemicals (NRA)NRA Chemical Review ProgramAustraliaGoogle Scholar
  33. Plaster, E J 1992Soil Science and Management. Second EditionDelmar Publishers IncAlbany, New York, USAGoogle Scholar
  34. Popov V, Sun H and Cornish P S 2004 Infiltration and adsorption both reduce pollutant loads in vegetative buffers. In Proceedings of American Water Resources Association (AWRA) Summer Specialty Conference on Riparian Ecosystems and Buffers: Multi-Scale Structure, Function, and Management, Olympic Valley CA, USAGoogle Scholar
  35. Popov V, Cornish P S, Sultana K and Morris E C 2005 “Atrazine degradation in soils: the role of microbial communities, atrazine application history and soil carbon”. Aus. J. Soil Res. 43, (In press)Google Scholar
  36. Rankins, A,Jr, Shaw, D R, Boyette, M, Seifert, S M 1998Minimising herbicide and sediment losses in runoff from vegetative filter stripsAbstr. Weed Sci. Soc. Am.3859Google Scholar
  37. Schreiber U and Bilger W 1986 Rapid assessment of stress effect on plant leaves by chlorophyll fluorescence measurements. NATO workshop, Sesimbra, Portugal 1985Google Scholar
  38. Sengalevitch G, Kuzmanov N, Alandjiiski D and Kostadinova P 1987 Triazine pollution of soil (in Bulgarian). J. Agr. Sci. 1/1987 (Abstract in English), BulgariaGoogle Scholar
  39. Stafford, J L 1991Techniques for the establishment of kangaroo grass in South Australian conservation reservesPlant Prot. Quart.6120122Google Scholar
  40. USA Environmental Protection Agency (Office of Pesticides Programs) 2002 HED’s revised preliminary human health risk assessment for the re-registration eligibility decision (RED), Available on-line: Scholar
  41. USDA (United States Department of Agriculture) 2000 Conservation buffers to reduce pesticide losses. National Resource Conservation Service (NCRS), USA. Available On-line: Scholar
  42. Vanderheyden, V, Debongnie, P, Pussemier, L 1997Accelerated degradation and mineralisation of atrazine in surface and subsurface soil materialsPestic. Sci.49237242CrossRefGoogle Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  1. 1.Landscape and Ecosystem Management, School of Environment and AgricultureUniversity of Western SydneyPenrith SouthAustralia

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