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Site-specific regulation of transcriptional responses to cadmium stress in the hyperaccumulator, Sedum alfredii: based on stem parenchymal and vascular cells

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Abstract

Key message

We compared the transcriptomes of parenchymal and vascular cells of Sedum alfredii stem under Cd stress to reveal gene regulatory networks underlying Cd hyperaccumulation.

Abstract

Cadmium (Cd) hyperaccumulation in plants is a complex biological process controlled by gene regulatory networks. Efficient transport through vascular systems and storage by parenchymal cells are vital for Cd hyperaccumulation in the Cd hyperaccumulator Sedum alfredii, but the genes involved are poorly understood. We investigated the spatial gene expression profiles of transport and storage sites in S. alfredii stem using laser-capture microdissection coupled with RNA sequencing. Gene expression patterns in response to Cd were distinct in vascular and parenchymal cells, indicating functional divisions that corresponded to Cd transportation and storage, respectively. In vascular cells, plasma membrane-related terms enriched a large number of differentially-expressed genes (DEGs) for foundational roles in Cd transportation. Parenchymal cells contained considerable DEGs specifically concentrated on vacuole-related terms associated with Cd sequestration and detoxification. In both cell types, DEGs were classified into different metabolic pathways in a similar way, indicating the role of Cd in activating a systemic stress signalling network where ATP-binding cassette transporters and Ca2+ signal pathways were probably involved. This study identified site-specific regulation of transcriptional responses to Cd stress in S. alfredii and analysed a collection of genes that possibly function in Cd transportation and detoxification, thus providing systemic information and direction for further investigation of Cd hyperaccumulation molecular mechanisms.

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Data availability

The sequence data of the current study has been submitted to the SRA database (SRA accession: SRP155284).

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Acknowledgements

The investigation of Cd distribution imaging by µ-XRF was conducted at the Advanced Photon Source, USA (Proposal No.: 23899) and we thank all the staff there. We also sincerely thank Vazyme Biotech (Nanjing, China) for their RNA sequencing service.

Funding

This work was supported by National Natural Science Foundation of China projects (31672235, 31471939), and the project from National Key Research and Development Program of China (2016YFD0800401).

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Authors and Affiliations

  1. Zhejiang Provincial Key Laboratory of Subtropic Soil and Plant Nutrition, College of Environmental & Resource Science, Zhejiang University, Hangzhou, 310058, China

    Yan Hu, Lingling Xu, Shengke Tian, Lingli Lu & Xianyong Lin

  2. Key Laboratory of Environment Remediation and Ecological Health, College of Environmental & Resource Science, Zhejiang University, Hangzhou, 310058, China

    Yan Hu, Lingling Xu, Shengke Tian, Lingli Lu & Xianyong Lin

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  1. Yan Hu
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LL supervised and designed the experiments; YH and LX performed most of the experiments and analysed data with contributions from ST; YH and LL conceived the project and wrote the manuscript with contributions from XL and ST.

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Correspondence to Lingli Lu.

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Hu, Y., Xu, L., Tian, S. et al. Site-specific regulation of transcriptional responses to cadmium stress in the hyperaccumulator, Sedum alfredii: based on stem parenchymal and vascular cells. Plant Mol Biol 99, 347–362 (2019). https://doi.org/10.1007/s11103-019-00821-1

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