Plant Molecular Biology

, Volume 95, Issue 4–5, pp 441–449 | Cite as

Lipid droplet-associated gene expression and chromatin remodelling in LIPASE 5′-upstream region from beginning- to mid-endodormant bud in ‘Fuji’ apple

  • Takanori Saito
  • Shanshan Wang
  • Katsuya Ohkawa
  • Hitoshi Ohara
  • Hiromi Ikeura
  • Yukiharu Ogawa
  • Satoru Kondo


Key message

We found that lipid accumulation in the meristem region and the expression of MdLIP2A, which appears to be regulated by chromatin remodeling, coincided with endodormancy induction in the ‘Fuji’ apple.


In deciduous trees, including apples (Malus × domestica Borkh.), lipid accumulation in the meristem region towards endodormancy induction has been thought to be an important process for the acquisition of cold tolerance. In this study, we conducted histological staining of crude lipids in the meristem region of ‘Fuji’ apples and found that lipid accumulation coincided with endodormancy induction. Since a major component of lipid bodies (triacylglycerol) is esterified fatty acids, we analysed fatty acid-derived volatile compounds and genes encoding fatty acid-modifying enzymes (MdLOX1A and MdHPL2A); the reduction of lipid breakdown also coincided with endodormancy induction. We then characterised the expression patterns of lipid body-regulatory genes MdOLE1 and MdLIP2A during endodormancy induction and found that the expression of MdLIP2A correlated well with lipid accumulation towards endodormancy induction. Based on these results, we conducted chromatin remodelling studies and localized the cis-element in the 5′-upstream region of MdLIP2A to clarify its regulatory mechanism. Finally, we revealed that chromatin was concentrated − 764 to − 862 bp of the 5′-upstream region of MdLIP2A, which harbours the GARE [gibberellin responsive MYB transcription factor binding site] and CArG [MADS-box transcription factor binding site] motifs—meristem development-related protein-binding sites.


CHART-PCR Chromatin remodelling Endodormancy induction Lipid body 



MADS-box transcription factor-binding site


Dormancy-associated MADS-box


Elongation factor 1




Gibberellin-responsive MYB transcription factor-binding site


Genome Database for Rosaceae


National Center for Biotechnology Information


Quantitative trait locus





This work was supported by the Program to Disseminate Tenure Tracking System from the Japanese Ministry of Education, Culture, Sports, Science and Technology, and by a grant from the Chiba University to TS.

Author contributions

TS designed the study and wrote paper, WS and YO performed and supervised the microscopy experiments, respectively, HI performed GC/MS analysis, OK, HO, and SK revised the article and corrected the content.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest with the contents of this article.

Supplementary material

11103_2017_662_MOESM1_ESM.pdf (296 kb)
Supplementary material 1 (PDF 295 KB)


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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  • Takanori Saito
    • 1
  • Shanshan Wang
    • 1
  • Katsuya Ohkawa
    • 1
  • Hitoshi Ohara
    • 1
    • 2
  • Hiromi Ikeura
    • 3
  • Yukiharu Ogawa
    • 1
  • Satoru Kondo
    • 1
  1. 1.Graduate School of HorticultureChiba UniversityMatsudoJapan
  2. 2.Center for Environment, Health and Field SciencesChiba UniversityKashiwa-no-haJapan
  3. 3.Organization for the Strategic Coordination of Research and Intellectual PropertiesMeiji UniversityKawasakiJapan

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