Plant Molecular Biology

, Volume 95, Issue 1–2, pp 181–197 | Cite as

Genome-wide analysis of GDSL-type esterases/lipases in Arabidopsis

  • Chia-Ping Lai
  • Li-Min Huang
  • Long-Fang O. Chen
  • Ming-Tsair Chan
  • Jei-Fu Shaw
Article

Abstract

Key message

In this present study, we introduce a fundamental framework and provide information regarding the possible roles of GDSL-type esterase/lipase gene family in Arabidopsis.

Abstract

GDSL-type esterases/lipases are hydrolytic enzymes with multifunctional properties such as broad substrate specificity, regiospecificity, and stereoselectivity. In this study, we identified 105 GDSL-type esterase/lipase genes in Arabidopsis thaliana by conducting a comprehensive computational analysis. Expression studies indicated that GDSL-type lipase proteins showed varied expression patterns. Phylogenetic tree analysis indicated that AtGELP (Arabidopsis thaliana GDSL-type esterase/lipase protein) gene family was divided into four clades. The phylogenetic analysis, combined with protein motif architectures, and expression profiling were used to predict the roles AtGELP genes. To investigate the physical roles of the AtGELP gene family, we successfully screened 88 AtGELP T-DNA knockout lines for 54 AtGELP genes from 199 putative SALK T-DNA mutants. Transgenic plants of AtGELP genes were used to elucidate the phenotypic characteristics in various developmental stages or stress conditions. Our results suggest that the AtGELP genes have diverse physical functions such as affecting the germination rate and early growth of seedlings subjected to high concentrations of glucose, or being involved in biotic stress responses.

Keywords

GDSL Lipase Esterase Genome-wide analysis Arabidopsis 

Notes

Acknowledgements

This research was supported by a Grant from the National Science Council of Taiwan Number NSC 102-2313-B-269-001 to C. P. Lai.

Author Contributions

CPL and LMH supervised the research design, performed research, and analyzed the data. MTC and LFOC designed research and analyzed the data. JFS supervised the study, designed research, analyzed data, and drafted the manuscript. All authors approved the final manuscript.

Supplementary material

11103_2017_648_MOESM1_ESM.pdf (394 kb)
Supplementary material 1 (PDF 393 KB)
11103_2017_648_MOESM2_ESM.ppt (440 kb)
Supplementary material 2 (PPT 440 KB)

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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  • Chia-Ping Lai
    • 1
  • Li-Min Huang
    • 2
    • 6
  • Long-Fang O. Chen
    • 2
    • 3
  • Ming-Tsair Chan
    • 2
    • 5
    • 6
  • Jei-Fu Shaw
    • 2
    • 4
    • 5
    • 7
  1. 1.Department of Food and Beverage ManagementFar East UniversityTainanTaiwan, Republic of China
  2. 2.Institute of BiotechnologyNational Cheng Kung UniversityTainanTaiwan, Republic of China
  3. 3.Institute of Plant and Microbial BiologyAcademia SinicaTaipeiTaiwan, Republic of China
  4. 4.Department of Biological Science and TechnologyI-Shou UniversityKaohsiungTaiwan, Republic of China
  5. 5.Agricultural Biotechnology Research CenterAcademia SinicaTaipeiTaiwan, Republic of China
  6. 6.Academia Sinica Biotechnology Center in Southern TaiwanTainanTaiwan, Republic of China
  7. 7.Agricultural Biotechnology CenterNational Chung Hsing UniversityTaichungTaiwan, Republic of China

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