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Plant Molecular Biology

, Volume 93, Issue 3, pp 247–267 | Cite as

Metabolo-transcriptome profiling of barley reveals induction of chitin elicitor receptor kinase gene (HvCERK1) conferring resistance against Fusarium graminearum

  • Shailesh Karre
  • Arun Kumar
  • Dhananjay Dhokane
  • Ajjamada C. Kushalappa
Article

Abstract

Key message

We report plausible disease resistance mechanisms induced by barley resistant genotype CI89831 against Fusarium head blight (FHB) based on metabolo-transcriptomics approach. We identified HvCERK1 as a candidate gene for FHB resistance, which is functional in resistant genotype CI9831 but non-functional in susceptible cultivars H106-371 and Zhedar-2. For the first time, we were able to show a hierarchy of regulatory genes that regulated downstream biosynthetic genes that eventually produced resistance related metabolites that reinforce the cell walls to contain the pathogen progress in plant. The HvCERK1 can be used for replacing in susceptible commercial cultivars, if non-functional, based on genome editing.

Abstract

Fusarium head blight (FHB) management is a great challenge in barley and wheat production worldwide. Though barley genome sequence and advanced omics technologies are available, till date none of the resistance mechanisms has been clearly deciphered. Hence, this study was aimed at identifying candidate gene(s) and elucidating resistance mechanisms induced by barley resistant genotype CI9831 based on integrated metabolomics and transcriptomics approach. Following Fusarium graminearum infection, we identified accumulation of specific set of induced secondary metabolites, belonging to phenylpropanoid, hydroxycinnamic acid (HCAA) and jasmonic acid pathways, and their biosynthetic genes. In association with these, receptor kinases such as chitin elicitor receptor kinase (HvCERK1) and protein kinases such as MAP kinase 3 (HvMPK3) and MAPK substrate 1 (HvMKS1), and transcription factors such as HvERF1/5, HvNAC42, HvWRKY23 and HvWRKY70 were also found upregulated with high fold change. Polymorphism studies across three barley genotypes confirmed the presence of mutations in HvCERK1 gene in two susceptible genotypes, isolating this gene as a potential candidate for FHB resistance. Further, the silencing of functional HvCERK1 gene in the resistant genotype CI9831, followed by gene expression and metabolite analysis revealed its role as an elicitor recognition receptor that triggered downstream regulatory genes, which in turn, regulated downstream metabolic pathway genes to biosynthesize resistance related (RR) metabolites to contain the pathogen to spikelet infection. A putative model on metabolic pathway regulation is proposed.

Keywords

Fusarium graminearum HvCERK1 HvMKS1 Hydroxycinnamic acid amides (HCAAs) Metabolo-transcriptomics Phenylpropanoids 

Notes

Acknowledgements

We acknowledge the financial support of Ministère de l’Agriculture, des Pêcheries et de l’Alimentation du Québec (MAPAQ), Québec, Canada, and Natural Sciences and Engineering Research Council of Canada (NSERC). We thank Dr. TM Choo, AAFC, for providing the seeds of barley genotypes used here.

Author contributions

SK, conducted the experiment, analyzed the data, wrote the manuscript; AK, DD helped in analyzing metabolite and transcriptome data, and developing figures; ACK*, conceived the idea, aided in designing the experiment and edited the manuscript.

Supplementary material

11103_2016_559_MOESM1_ESM.pdf (2.5 mb)
Supplementary material 1 (PDF 2527 KB)

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Shailesh Karre
    • 1
  • Arun Kumar
    • 1
  • Dhananjay Dhokane
    • 1
  • Ajjamada C. Kushalappa
    • 1
  1. 1.Department of Plant ScienceMcGill UniversitySainte-Anne-de-BellevueCanada

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