Plant Molecular Biology

, Volume 87, Issue 1–2, pp 47–67 | Cite as

Overexpression of a cotton annexin gene, GhAnn1, enhances drought and salt stress tolerance in transgenic cotton

  • Feng Zhang
  • Shufen Li
  • Shuming Yang
  • Like Wang
  • Wangzhen Guo


Plant annexins are members of a diverse, multigene protein family that has been associated with a variety of cellular processes and responses to abiotic stresses. GhAnn1, which encodes a putative annexin protein, was isolated from a cotton (Gossypium hirsutum L. acc 7235) cDNA library. Tissue-specific expression showed that GhAnn1 is expressed at differential levels in all tissues examined and strongly induced by various phytohormones and abiotic stress. In vivo and in vitro subcellular localization suggested that GhAnn1 is located in the plasma membrane. In response to drought and salt stress, transgenic cotton plants overexpressing GhAnn1 showed significantly higher germination rates, longer roots, and more vigorous growth than wild-type plants. In addition, plants overexpressing GhAnn1 had higher total chlorophyll content, lower lipid peroxidation levels, increased peroxidase activities, and higher levels of proline and soluble sugars, all of which contributed to increased salt and drought stress tolerance. However, transgenic cotton plants in which the expression of GhAnn1 was suppressed showed the opposite results compared to the overexpressing plants. These findings demonstrated that GhAnn1 plays an important role in the abiotic stress response, and that overexpression of GhAnn1 in transgenic cotton improves salt and drought tolerance.


Cotton Gossypium hirsutum GhAnn1 Drought stress Salt stress Abiotic stress tolerance 



This program was financially supported in part by National Science Foundation in China (31171590), the National Transgenic Program (2011ZX08005-004), Jiangsu Agriculture Science and Technology Innovation Fund (CX(14)2065), and a project funded by PAPD-JHEI and JCIC-MCP.

Conflict of interest

The authors have declared that no competing interests exist.

Supplementary material

11103_2014_260_MOESM1_ESM.tif (90 kb)
Intron–exon structures of 14 annexin genes from G. raimondii. The scale bar represents 0.2 amino acid substitutions per site. Gene structures were drawn using the online tool Gene Structure Display Server ( (TIFF 90 kb)
11103_2014_260_MOESM2_ESM.tif (366 kb)
Characterization of transgenic cotton plants. A: PCR analysis of GhAnn1 transgenic plants using NPTII-specific and promoter-gene primers, respectively. M: marker DL2000. P: positive control. WT, wild-type. a - GhAnn1 gene sense vector. b - GhAnn1 gene antisense vector. c - GhAnn1 3′ fragment antisense vector. B: PCR analysis of the NPTII gene. C: Southern blot analysis of T3 transgenic cotton plants for transgene copy number using the restriction enzyme EcoRI, which has a single recognition site in the T-DNA. 1 to 8 - GhAnn1 overexpressing transgenic lines 1 to 8. (TIFF 366 kb)
11103_2014_260_MOESM3_ESM.docx (27 kb)
Supplementary material 3 (DOCX 26 kb)
11103_2014_260_MOESM4_ESM.docx (25 kb)
Supplementary material 4 (DOCX 24 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Feng Zhang
    • 1
  • Shufen Li
    • 1
  • Shuming Yang
    • 1
  • Like Wang
    • 1
  • Wangzhen Guo
    • 1
  1. 1.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Hybrid Cotton R & D Engineering Research Center, MOENanjing Agricultural UniversityNanjingPeople’s Republic of China

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