Overexpression of a cotton annexin gene, GhAnn1, enhances drought and salt stress tolerance in transgenic cotton
- 1.7k Downloads
Plant annexins are members of a diverse, multigene protein family that has been associated with a variety of cellular processes and responses to abiotic stresses. GhAnn1, which encodes a putative annexin protein, was isolated from a cotton (Gossypium hirsutum L. acc 7235) cDNA library. Tissue-specific expression showed that GhAnn1 is expressed at differential levels in all tissues examined and strongly induced by various phytohormones and abiotic stress. In vivo and in vitro subcellular localization suggested that GhAnn1 is located in the plasma membrane. In response to drought and salt stress, transgenic cotton plants overexpressing GhAnn1 showed significantly higher germination rates, longer roots, and more vigorous growth than wild-type plants. In addition, plants overexpressing GhAnn1 had higher total chlorophyll content, lower lipid peroxidation levels, increased peroxidase activities, and higher levels of proline and soluble sugars, all of which contributed to increased salt and drought stress tolerance. However, transgenic cotton plants in which the expression of GhAnn1 was suppressed showed the opposite results compared to the overexpressing plants. These findings demonstrated that GhAnn1 plays an important role in the abiotic stress response, and that overexpression of GhAnn1 in transgenic cotton improves salt and drought tolerance.
KeywordsCotton Gossypium hirsutum GhAnn1 Drought stress Salt stress Abiotic stress tolerance
This program was financially supported in part by National Science Foundation in China (31171590), the National Transgenic Program (2011ZX08005-004), Jiangsu Agriculture Science and Technology Innovation Fund (CX(14)2065), and a project funded by PAPD-JHEI and JCIC-MCP.
Conflict of interest
The authors have declared that no competing interests exist.
- Anjum F, Yaseen M, Rasool E, Wahid A, Anjum S (2003) Water stress in barley (hordeum vulgare): I. Effect on morpohological characters. Seeds 105:266–271Google Scholar
- Chyzhykova O, Palladina T (2005) The role of amino acids and sugars in supporting of osmotic homeostasis in maize seedlings under salinization conditions and treatment with synthetic growth regulators. Ukr Biokhim Zh 78:124–129Google Scholar
- He YJ, Guo WZ, Zhang TZ (2008) Molecular cloning, characterization and mapping of GhLipase gene in Gossypium hirsutum. J Agric Biotechnol 17:84–86Google Scholar
- Hubbard KE, Nishimura N, Hitomi K, Getzoff ED, Schroeder JI (2010) Earlyabscisic acid signal transduction mechanisms: newly discovered components and newly emerging questions. Gene Dev 24:1695–1708Google Scholar
- Hung SH, Yu CW, Lin CH (2005) Hydrogen peroxide functions as a stress signal in plants. Bot Bull Acad Sin 46:1–10Google Scholar
- Jiang J, Zhang T (2003) Extraction of total RNA in cotton tissues with CTAB-acidic phenolic method. Cotton Sci 15:166–167Google Scholar
- Ketting RF, Tijsterman M, Plasterk RH (2006) Cosuppression in C. elegans. CSH Protoc 1. doi: 10.1101/pdb.prot4318
- Massacci A, Nabiev S, Pietrosanti L, Nematov S, Chernikova T, Thor K, Leipner J (2008) Response of the photosynthetic apparatus of cotton (Gossypium hirsutum) to the onset of drought stress under field conditions studied by gas-exchange analysis and chlorophyll fluorescence imaging. Plant Physiol Biochem 46:189–195PubMedCrossRefGoogle Scholar
- Paterson AH, Brubaker C, Wendel JF (1993) A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP or PCR analysis. Plant Mol Bio Rep 11:122–127Google Scholar
- Qian SY, Huang JQ, Peng YJ, Zhou BL, Ying MC, Shen DZ, Liu GL, Hu TX, Xu YJ, Gu LM, Ni WC, Chen S (1992) Studies on the hybrid of G. hirsutum L and G. anomalum Wawr. & Peyr. and application in breeding. Sci Agric Sin 25:44–51Google Scholar