Expression of Arabidopsis sugar transport protein STP13 differentially affects glucose transport activity and basal resistance to Botrytis cinerea
- 1.4k Downloads
Botrytis cinerea is the causing agent of the grey mold disease in more than 200 crop species. While signaling pathways leading to the basal resistance against this fungus are well described, the role of the import of sugars into host cells remains to be investigated. In Arabidopsis thaliana, apoplastic hexose retrieval is mediated by the activity of sugar transport proteins (STPs). Expression analysis of the 14 STP genes revealed that only STP13 was induced in leaves challenged with B. cinerea. STP13-modified plants were produced and assayed for their resistance to B. cinerea and glucose transport activity. We report that STP13-deficient plants exhibited an enhanced susceptibility and a reduced rate of glucose uptake. Conversely, plants with a high constitutive level of STP13 protein displayed an improved capacity to absorb glucose and an enhanced resistance phenotype. The correlation between STP13 transcripts, protein accumulation, glucose uptake rate and resistance level indicates that STP13 contributes to the basal resistance to B. cinerea by limiting symptom development and points out the importance of the host intracellular sugar uptake in this process. We postulate that STP13 would participate in the active resorption of hexoses to support the increased energy demand to trigger plant defense reactions and to deprive the fungus by changing sugar fluxes toward host cells.
KeywordsSugar transporter protein Arabidopsis thaliana Botrytis cinerea Basal resistance Plant–pathogen interactions
Pauline Lemonnier and Florian Veillet are supported by Grants from the French Ministry of Higher Education and Research, and the Région Poitou–Charentes, respectively. We are grateful to Vincent Lebeurre and Bruno Faure for helping us producing numerous plants used in this study. Antoine Plasseraud Desgranges is acknowledged for his help in the correction of the manuscript. We would like to thank all our colleagues for inspiring discussions. The CNRS, the University of Poitiers and the Région Poitou–Charentes are gratefully acknowledged for their financial support.
- Alonso JM, Stepanova AN, Leisse TJ, Kim CJ, Chen H, Shinn P, Stevenson DK, Zimmerman J, Barajas P, Cheuk R, Gadrinab C, Heller C, Jeske A, Koesema E, Meyers CC, Parker H, Prednis L, Ansari Y, Choy N, Deen H, Geralt M, Hazari N, Hom E, Karnes M, Mulholland C, Ndubaku R, Schmidt I, Guzman P, Aguilar-Henonin L, Schmid M, Weigel D, Carter DE, Marchand T, Risseeuw E, Brogden D, Zeko A, Crosby WL, Berry CC, Ecker JR (2003) Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653–657PubMedCrossRefGoogle Scholar
- Arbelet D, Malfatti P, Simond-Côte E, Fontaine T, Desquilbet L, Expert D, Kunz C, Soulié M-C (2010) Disruption of the Bcchs3a Chitin Synthase gene in Botrytis cinerea is responsible for altered adhesion and overstimulation of host plant immunity. Mol Plant Microbe Interact 23(10):1324–1334PubMedCrossRefGoogle Scholar
- Fotopoulos V, Gilbert MJ, Pittman JK, Marvier AC, Buchanan AJ, Sauer N, Hall JL, Williams LE (2003) The monosaccharide transporter gene, AtSTP4, and the cell-wall invertase, Atbetafruct1, are induced in Arabidopsis during infection with the fungal biotroph Erysiphe cichoracearum. Plant Physiol 132(2):821–829PubMedCentralPubMedCrossRefGoogle Scholar
- Lemoine R, La Camera S, Atanassova R, Dédaldéchamp F, Allario T, Pourtau N, Bonnemain J-L, Laloi M, Coutos-Thévenot P, Maurousset L, Faucher M, Girousse C, Lemonnier P, Parrilla J, Durand M (2013) Source to sink transport and regulation by environmental factors. Front Plant Sci 4:272 Google Scholar
- Thomma BPHJ, Eggermont K, Penninckx IAMA, Mauch-Mani B, Vogelsang R, Cammue BPA, Broekaert WF (1998) Separate jasmonate-dependent and salicylate-dependent defense-response pathways in Arabidopsis are essential for resistance to distinct microbial pathogens. Proc Natl Acad Sci USA 95(25):15107–15111PubMedCentralPubMedCrossRefGoogle Scholar
- Truernit E, Schmid J, Epple P, Illig J, Sauer N (1996) The sink-specific and stress-regulated Arabidopsis STP4 gene: enhanced expression of a gene encoding a monosaccharide transporter by wounding, elicitors, and pathogen challenge. Plant Cell 8(12):2169–2182PubMedCentralPubMedCrossRefGoogle Scholar