Plant Molecular Biology

, Volume 83, Issue 4–5, pp 445–458 | Cite as

The roles of two transcription factors, ABI4 and CBFA, in ABA and plastid signalling and stress responses

  • Zhong-Wei Zhang
  • Ling-Yang Feng
  • Jian Cheng
  • He Tang
  • Fei Xu
  • Feng Zhu
  • Zhong-Yi Zhao
  • Ming Yuan
  • Yang-Er Chen
  • Jian-Hui Wang
  • Shu Yuan
  • Hong-Hui Lin


Genetic and physiological studies have revealed evidences for multiple signaling pathways by which the plastid exerts retrograde control over photosynthesis-associated-nuclear-genes. In this study we have examined the mechanisms of control of transcription by plastid signals, focusing on transcription factors. We have also further addressed the physical nature of plastid signals and the physiological role, in stress acclimation of this regulatory pathway. ABI4, a master Apetala 2 (AP2)-type transcription factor (TF), is targeted by multiple signalling pathways in plant cells, such as abscisic acid (ABA) signals, sugar signals and plastid signals derived from reactive oxygen species (ROS) and chlorophyll intermediates. ABI4 binds the promoter of target genes to prevent their transcription by competing with other competitive TFs. However, we found that once ABI4 bound the element (CCACGT), it may not be bound by other TFs, therefore making the signalling long-lasting. Downstream of ABI4, CBFA (CCAAT binding factor A) is a subunit of the HAP2/HAP3/HAP5 (Heme activator protein) trimeric transcription complex. CBFA however is a redundant HAP3 subunit. When emergency occurs (such as herbicide treatments or environmental stresses followed by ABA and ROS accumulation), the master transcription factor ABI4 down-regulates some TFs, like CBFA, and then some other TF subunits enter the transcription complex and transcriptional efficiency of stress-responsive genes (including the transcription co-factor CBP) is improved instantaneously. abi4, cbfA and cbp mutants showed weaker drought-tolerance after a herbicide norflurazon treatment, which indicated the physiological role of these key transcription factors.


Transcription factor ABI4 CBFA GUN1 Plastid signaling Tetrapyrroles 



Abscisic acid


Abscisic acid insensitive 4


CCAAT binding factor A


Transcription co-factor CAAT binding protein


Chromatin immunoprecipitation


Genomes uncoupled 1


High light



Mg-Proto IX

Mg-protoporphyrin IX




Photosynthetic electron transport chain


Plastid gene expression




Reactive oxygen species


Transcription factor



We thank Prof. Joanne Chory (The Salk Institute, La Jolla, USA) for gun1-9 seeds. We thank Dr. Xiao-Chao Xu (College of Bioindustry, Chengdu University, China) for technical assistance with ChIP assay and antibody preparation. This work was supported by the National Nature Science Foundation of China (31070210, 91017004 and 30970214), the National Key Basic Research ‘973’ Program of China (2009CB118500), and the Doctoral Foundation of the Ministry of Education (20110181110059).

Supplementary material

11103_2013_102_MOESM1_ESM.pdf (1.2 mb)
Supplementary material 1 (PDF 1184 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Zhong-Wei Zhang
    • 1
  • Ling-Yang Feng
    • 1
  • Jian Cheng
    • 2
  • He Tang
    • 2
  • Fei Xu
    • 2
  • Feng Zhu
    • 2
  • Zhong-Yi Zhao
    • 2
  • Ming Yuan
    • 3
  • Yang-Er Chen
    • 3
  • Jian-Hui Wang
    • 4
  • Shu Yuan
    • 1
  • Hong-Hui Lin
    • 2
  1. 1.College of Resources and Environmental SciencesSichuan Agricultural UniversityChengduChina
  2. 2.Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life ScienceSichuan UniversityChengduChina
  3. 3.College of Biology and ScienceSichuan Agricultural UniversityYa’anChina
  4. 4.Horticulture InstituteSichuan Academy of Agricultural SciencesChengduChina

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