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Plant Molecular Biology

, Volume 78, Issue 3, pp 247–258 | Cite as

C2 domain is responsible for targeting rice phosphoinositide specific phospholipase C

  • Sunny D. Rupwate
  • Ram Rajasekharan
Article

Abstract

Phosphoinositide-specific phospholipase C (PLC) is involved in Ca2+ mediated signalling events that lead to altered cellular status. Using various sequence-analysis methods, we identified two conserved motifs in known PLC sequences. The identified motifs are located in the C2 domain of plant PLCs and are not found in any other protein. These motifs are specifically found in the Ca2+ binding loops and form adjoining beta strands. Further, we identified certain conserved residues that are highly distinct from corresponding residues of animal PLCs. The motifs reported here could be used to annotate plant-specific phospholipase C sequences. Furthermore, we demonstrated that the C2 domain alone is capable of targeting PLC to the membrane in response to a Ca2+ signal. We also showed that the binding event results from a change in the hydrophobicity of the C2 domain upon Ca2+ binding. Bioinformatic analyses revealed that all PLCs from Arabidopsis and rice lack a transmembrane domain, myristoylation and GPI-anchor protein modifications. Our bioinformatic study indicates that plant PLCs are located in the cytoplasm, the nucleus and the mitochondria. Our results suggest that there are no distinct isoforms of plant PLCs, as have been proposed to exist in the soluble and membrane associated fractions. The same isoform could potentially be present in both subcellular fractions, depending on the calcium level of the cytosol. Overall, these data suggest that the C2 domain of PLC plays a vital role in calcium signalling.

Keywords

Plant phosphoplipase C C2 domain Membrane targeting Surface hydrophobicity Phospholipids 

Abbreviations

PLC

Phosphoinositide-specific phospholipase C

PIP2

Phosphatidylinositol (4,5)-bisphosphate

IP3

Inositol 1,4,5-triphosphate

DAG

Diacylglycerol

PH

Pleckstrin homology

SH

Src homology

PS

Phosphatidylserine

PC

Phosphatidylcholine

PE

Phosphatidylethanolamine

PI

Phosphatidylinositol

SUV

Small unilamellar vesicles

LMV

Large multilamellar vesicles

ANS

1-Anilino-8-naphthalene sulfonate

Notes

Acknowledgments

Sunny D. Rupwate is recipients of Council of Scientific and Industrial Research Fellowship, New Delhi, India. This research was supported by a grant from Council of Scientific and Industrial Research, New Delhi and Department of Biotechnology, Government of India, New Delhi.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.Department of BiochemistryIndian Institute of ScienceBangaloreIndia
  2. 2.Central Institute of Medicinal and Aromatic PlantsCouncil of Scientific and Industrial ResearchLucknowIndia

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