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Plant Molecular Biology

, Volume 76, Issue 3–5, pp 371–384 | Cite as

Modification of reactive oxygen species scavenging capacity of chloroplasts through plastid transformation

  • Miranda Poage
  • Bénédicte Le Martret
  • Marcel A. K. Jansen
  • Gregory D. Nugent
  • Philip J. Dix
Article

Abstract

Reactive oxygen species (ROS), including superoxide anions, hydrogen peroxide and hydroxyl radicals are generated through normal biochemical processes, but their production is increased by abiotic stresses. The prospects for enhancing ROS scavenging, and hence stress tolerance, by direct gene expression in a vulnerable cell compartment, the chloroplast, have been explored in tobacco. Several plastid transformants were generated which contained either a Nicotiana mitochondrial superoxide dismutase (MnSOD) or an Escherichia coli glutathione reductase (gor) gene. MnSOD lines had a three-fold increase in MnSOD activity, but interestingly a five to nine-fold increase in total chloroplast SOD activity. Gor transgenic lines had up to 6 times higher GR activity and up to 8 times total glutathione levels compared to wild type tobacco. Photosynthetic capacity of transplastomic plants, as measured by chlorophyll content and variable fluorescence of PSII was equivalent to non-transformed plants. The response of these transplastomic lines to several applied stresses was examined. In a number of cases improved stress tolerance was observed. Examples include enhanced methyl viologen (Paraquat)-induced oxidative stress tolerance in Mn-superoxidase dismutase over-expressing plants, improved heavy metal tolerance in glutathione reductase expressing lines, and improved tolerance to UV-B radiation in both sets of plants.

Keywords

Chloroplast transformation Superoxide dismutase Glutathione reductase Abiotic stress 

Notes

Acknowledgments

This research was supported by grants from Science Foundation Ireland (grant: 06/RFP/EEB035) and Enterprise Ireland (grant: SC/2001/343). The authors would like to thank Manfred Klaas and Olga Grant for assistance in the preparation of the figures.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Miranda Poage
    • 1
  • Bénédicte Le Martret
    • 4
  • Marcel A. K. Jansen
    • 2
  • Gregory D. Nugent
    • 3
  • Philip J. Dix
    • 4
  1. 1.Department of BiologyMidland CollegeMidlandUSA
  2. 2.ZEPS Plant Ecophysiology Group, School of Biological, Earth and Environmental SciencesUniversity College CorkCorkIreland
  3. 3.School of Applied Sciences, Biotechnology and Environmental BiologyRMIT UniversityMelbourneAustralia
  4. 4.Biology DepartmentNational University of Ireland MaynoothMaynoothIreland

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