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Plant Molecular Biology

, Volume 70, Issue 4, pp 403–420 | Cite as

Shared and novel molecular responses of mandarin to drought

  • Jacinta Gimeno
  • José Gadea
  • Javier Forment
  • Jorge Pérez-Valle
  • Julia Santiago
  • María A. Martínez-Godoy
  • Lynne Yenush
  • José M. Bellés
  • Javier Brumós
  • José M. Colmenero-Flores
  • Manuel Talón
  • Ramón Serrano
Article

Abstract

Drought is the most important stress experienced by citrus crops. A citrus cDNA microarray of about 6.000 genes has been utilized to identify transcriptomic responses of mandarin to water stress. As observed in other plant species challenged with drought stress, key genes for lysine catabolism, proline and raffinose synthesis, hydrogen peroxide reduction, vacuolar malate transport, RCI2 proteolipids and defence proteins such as osmotin, dehydrins and heat-shock proteins are induced in mandarin. Also, some aquaporin genes are repressed. The osmolyte raffinose could be detected in stressed roots while the dehydrin COR15 protein only accumulated in stressed leaves but not in roots. Novel drought responses in mandarin include the induction of genes encoding a new miraculin isoform, chloroplast β-carotene hydroxylase, oleoyl desaturase, ribosomal protein RPS13A and protein kinase CTR1. These results suggest that drought tolerance in citrus may benefit from inhibition of proteolysis, activation of zeaxanthin and linolenoyl synthesis, reinforcement of ribosomal structure and down-regulation of the ethylene response.

Keywords

Citrus Water stress Transcriptomics Raffinose Dehydrin Ethylene response 

Notes

Acknowledgments

We thank María J. Rodrigo and Lorenzo Zacarías (IATA, Valencia) for the Escherichia coli M15 strain expressing CrCOR15. This work was funded by “Ministerio de Educación y Ciencia” (Madrid, BFU2005-06388-C04-01 and AGL2007-65437-C04-01 grants) and “Consellería de Agricultura, Pesca y Alimentación de la Generalitat Valenciana” (Valencia, “Proyecto Genoma Cítricos”).

Supplementary material

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Jacinta Gimeno
    • 1
  • José Gadea
    • 1
  • Javier Forment
    • 1
  • Jorge Pérez-Valle
    • 1
  • Julia Santiago
    • 1
  • María A. Martínez-Godoy
    • 1
  • Lynne Yenush
    • 1
  • José M. Bellés
    • 1
  • Javier Brumós
    • 2
  • José M. Colmenero-Flores
    • 2
  • Manuel Talón
    • 2
  • Ramón Serrano
    • 1
  1. 1.Instituto De Biología Molecular y Celular de PlantasUniversidad Politécnica de Valencia-CSICValenciaSpain
  2. 2.Centro de Genómica, Instituto Valenciano de Investigaciones AgrariasMoncadaValenciaSpain

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