Abstract
Damaged DNA Binding protein 1 (DDB1) is a conserved protein and a component of multiple cellular complexes. Arabidopsis has two homologues of DDB1: DDB1A and DDB1B. In this study we examine the role of DDB1A in Arabidopsis UV tolerance and DNA repair using a DDB1A null mutant (ddb1a) and overexpression lines. DDB1A overexpression lines showed higher levels of UV-resistance than wild-type in a range of assays as well as faster DNA repair. However a significant difference between wild-type plants and ddb1a mutants was only observed immediately following UV treatment in root length and photoproduct repair assays. DDB1A and DDB1B mRNA levels increased 3 h after UV exposure and DDB1A is required for UV regulation of DDB1B and DDB2 mRNA levels. In conclusion, while DDB1A is sufficient to increase Arabidopsis UV tolerance, it is only necessary for immediate response to UV damage.
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Abbreviations
- 6-4PPs:
-
Pyrimidine (6-4) pyrimidinone dimers
- CPDs:
-
Cyclobutane pyrimidine dimers
- CS:
-
Cockayne syndrome
- DDB:
-
Damaged DNA binding protein
- GGR:
-
Global genomic repair
- HA:
-
Haemagglutinin
- NER:
-
Nucleotide excision repair
- TCR:
-
Transcription-coupled repair
- UV:
-
Ultraviolet
- XP:
-
Xeroderma pigmentosa
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Acknowledgements
The authors thank Yu Zhang, Mehdi Sefidgar, David Collister, Lana Rosenfeld, Avril Hatherell and Mala Vijayakumar for technical assistance and David Bird and Fawzi Razem for critical suggestions. The work was supported by a grant from the Natural Sciences and Engineering Research Council of Canada to D.F.S.
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Al Khateeb, W.M., Schroeder, D.F. Overexpression of Arabidopsis damaged DNA binding protein 1A (DDB1A) enhances UV tolerance. Plant Mol Biol 70, 371–383 (2009). https://doi.org/10.1007/s11103-009-9479-9
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DOI: https://doi.org/10.1007/s11103-009-9479-9