Plant Molecular Biology

, Volume 70, Issue 4, pp 371–383 | Cite as

Overexpression of Arabidopsis damaged DNA binding protein 1A (DDB1A) enhances UV tolerance

  • Wesam M. Al Khateeb
  • Dana F. Schroeder


Damaged DNA Binding protein 1 (DDB1) is a conserved protein and a component of multiple cellular complexes. Arabidopsis has two homologues of DDB1: DDB1A and DDB1B. In this study we examine the role of DDB1A in Arabidopsis UV tolerance and DNA repair using a DDB1A null mutant (ddb1a) and overexpression lines. DDB1A overexpression lines showed higher levels of UV-resistance than wild-type in a range of assays as well as faster DNA repair. However a significant difference between wild-type plants and ddb1a mutants was only observed immediately following UV treatment in root length and photoproduct repair assays. DDB1A and DDB1B mRNA levels increased 3 h after UV exposure and DDB1A is required for UV regulation of DDB1B and DDB2 mRNA levels. In conclusion, while DDB1A is sufficient to increase Arabidopsis UV tolerance, it is only necessary for immediate response to UV damage.


DDB1A DNA repair Arabidopsis UV DDB1B DDB2 



Pyrimidine (6-4) pyrimidinone dimers


Cyclobutane pyrimidine dimers


Cockayne syndrome


Damaged DNA binding protein


Global genomic repair




Nucleotide excision repair


Transcription-coupled repair




Xeroderma pigmentosa



The authors thank Yu Zhang, Mehdi Sefidgar, David Collister, Lana Rosenfeld, Avril Hatherell and Mala Vijayakumar for technical assistance and David Bird and Fawzi Razem for critical suggestions. The work was supported by a grant from the Natural Sciences and Engineering Research Council of Canada to D.F.S.


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Department of Biological SciencesUniversity of ManitobaWinnipegCanada
  2. 2.Department of Biological SciencesYarmouk UniversityIrbidJordan

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