ROLLED LEAF 9, encoding a GARP protein, regulates the leaf abaxial cell fate in rice
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Leaves, the collective organ produced by the shoot apical meristem (SAM), are polarized along their adaxial–abaxial axis. In this study, we characterized two rice (Oryza sativa) allelic rolled-leaf mutants, rolled leaf 9-1 (rl9-1) and rl9-2, which display very similar phenotypes with completely adaxialized leaves and malformed spikelets. We cloned the RL9 gene by way of a map-based cloning strategy. Molecular studies have revealed that RL9 encodes a GARP protein, an orthologue of Arabidopsis KANADIs. RL9 is mainly expressed in roots, leaves, and flowers. The transient expression of a RL9–GFP (green fluorescent protein) fusion protein has indicated that RL9 protein is localized in the nucleus, suggesting that RL9 acts as a putative transcription factor.
KeywordsOryzaSativa L. Rolled leaf KANADI RL9
We are grateful to Dr. Liu Qiaoquan (Agricultural College of Yangzhou University) for kindly providing the rl9-2 mutant. This research was supported in part by grants from the Ministry of Science and Technology of China (No. 2005CB120804), the Jiangsu Innovation Talent Project (No. BK2006506) and the Postgraduate Science Innovation Plan of Jiangsu Province.
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