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Plant Molecular Biology

, Volume 68, Issue 1–2, pp 43–59 | Cite as

Identification of a novel group of putative Arabidopsis thaliana β-(1,3)-galactosyltransferases

  • Yongmei Qu
  • Jack Egelund
  • Paul R. Gilson
  • Fiona Houghton
  • Paul A. Gleeson
  • Carolyn J. Schultz
  • Antony Bacic
Article

Abstract

To begin biochemical and molecular studies on the biosynthesis of the type II arabinogalactan chains on arabinogalactan-proteins (AGPs), we adopted a bioinformatic approach to identify and systematically characterise the putative galactosyltransferases (GalTs) responsible for synthesizing the β-(1,3)-Gal linkage from CAZy GT-family-31 from Arabidopsis thaliana. These analyses confirmed that 20 members of the GT-31 family contained domains/motifs typical of biochemically characterised β-(1,3)-GTs from mammalian systems. Microarray data confirm that members of this family are expressed throughout all tissues making them likely candidates for the assembly of the ubiquitously found AGPs. One member, At1g77810, was selected for further analysis including location studies that confirmed its presence in the Golgi and preliminary enzyme substrate specificity studies that demonstrated β-(1,3)-GalT activity. This bioinformatic/molecular study of CAZy GT-family-31 was validated by the recent report of Strasser et al. (Plant Cell 19:2278–2292, 2007) that another member of this family (At1g26810; GALT1) encodes a β-(1,3)-GalT involved in the biosynthesis of the Lewis a epitope of N-glycans in Arabidopsis thaliana.

Keywords

Arabinogalactan-protein CAZy-database Galactosyltransferase Glycosyltransferase Golgi 

Abbreviations

AG

Arabinogalactan

AGP

Arabinogalactan-protein

Ara

Arabinose

Araf

Arabinofuranose

CRD

Carbohydrate recognition domain

ER

Endoplasmic reticulum

Gal

Galactose

Galp

Galactopyranose

GalNAcT

N-acetylgalactosaminyltransferase

GalT

Galactosyltransferase

GlcA

Glucuronic acid

GlcNAcT

N-acetylglucosaminosyltransferase

GPI

Glycosylphosphatidylinositol

GT

Glycosyltransferases

HRGP

Hydroxyproline-rich glycoproteins

Hyp

Hydroxyproline

TMD

Transmembrane domain

Notes

Acknowledgements

This work was supported by a grant from the Australian Research Council (Discovery Projects DP0343454, DP0663374). Yongmei Qu acknowledges the support of a University of Melbourne International Research Scholarship and a University of Melbourne Research Scholarship. Jack Egelund was supported by The Carlsberg Foundation. We acknowledge the excellent administrative support of Ms Joanne Noble.

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Yongmei Qu
    • 1
  • Jack Egelund
    • 2
  • Paul R. Gilson
    • 3
  • Fiona Houghton
    • 4
  • Paul A. Gleeson
    • 4
  • Carolyn J. Schultz
    • 5
  • Antony Bacic
    • 1
  1. 1.Plant Cell Biology Research Centre, School of BotanyThe University of MelbourneMelbourneAustralia
  2. 2.Department of Molecular BiologyUniversity of CopenhagenCopenhagenDenmark
  3. 3.Infection and Immunity GroupThe Walter and Eliza Hall Institute of Medical ResearchParkvilleAustralia
  4. 4.Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology InstituteThe University of MelbourneMelbourneAustralia
  5. 5.School of Agriculture, Food and WineThe University of AdelaideAdelaideAustralia

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