Genome-wide survey of the RIP domain family in Oryza sativa and their expression profiles under various abiotic and biotic stresses
- 343 Downloads
Ribosome-inactivating proteins (RIPs) are N-glycosidases that inhibit protein synthesis by depurinating rRNA. Despite their identification more than 25 years ago, little is known about their biological functions. Here, we report a genome-wide identification of the RIP family in rice based on the complete genome sequence analysis. Our data show that rice genome encodes at least 31 members of this family and they all belong to type 1 RIP genes. This family might have evolved in parallel to species evolution and genome-wide duplications represent the major mechanism for this family expansion. Subsequently, we analyzed their expression under biotic (bacteria and fungus infection), abiotic (cold, drought and salinity) and the phytohormone ABA treatment. These data showed that some members of this family were expressed in various tissues with differentiated expression abundances whereas several members showed no expression under normal growth conditions or various environmental stresses. On the other hand, the expression of many RIP members was regulated by various abiotic and biotic stresses. All these data suggested that specific members of the RIP family in rice might play important roles in biotic and abiotic stress-related biological processes and function as a regulator of various environmental cues and hormone signaling. They may be potentially useful in improving plant tolerance to various abiotic and biotic stresses by over-expressing or suppressing these genes.
KeywordsBiotic stress Abiotic stress Ectopic expression Ribosome-inactivating protein Rice
Xanthomonas oryzae pv oryzae
We would like to thank Dr. Parameswaran Sriram for his help in real-time PCR analysis.
- Barbieri L, Polito L, Bolognesi A, Ciani M, Pelosi E, Farini V, Jha AK, Sharma N, Vivanco JM, Chambery A et al (2006) Ribosome-inactivating proteins in edible plants and purification and characterization of a new ribosome-inactivating protein from Cucurbita moschata. Biochim Biophys Acta (BBA) 1760:783–792Google Scholar
- Ferreras JM, Barbieri L, Girbés T, Battelli MG, Rojo MA, Arias FJ, Rocher MA, Soriano F, Mendéz E, Stirpe F (1993) Distribution and properties of major ribosome-inactivating proteins (28 S rRNA N-glycosidases) of the plant Saponaria officinalis, L. (Caryophyllaceae). Biochim Biophys Acta 1216:31–42PubMedGoogle Scholar
- Girbes T, Ferreras JM, Arias FJ, Stirpe F (2004) Description, distribution, activity and phylogenetic relationship of ribosome-inactivating proteins in plants, fungi and bacteria. Mini Rev Med Chem 4:467–482Google Scholar
- Lafitte HR, Ismail A, Bennett J (2004) Abiotic stress tolerance in rice for Asia: progress and the future. Proceedings of the 4th international crop science congress, Brisbane, AustraliaGoogle Scholar
- Yu J, Wang J, Lin W, Li S, Li H, Zhou J, Ni P, Dong W, Hu S, Zeng C et al (2005) The genomes of Oryza sativa: a history of duplications. PLoS Biol 3(e38):0266–0281Google Scholar