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Genome-wide survey of the RIP domain family in Oryza sativa and their expression profiles under various abiotic and biotic stresses

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Abstract

Ribosome-inactivating proteins (RIPs) are N-glycosidases that inhibit protein synthesis by depurinating rRNA. Despite their identification more than 25 years ago, little is known about their biological functions. Here, we report a genome-wide identification of the RIP family in rice based on the complete genome sequence analysis. Our data show that rice genome encodes at least 31 members of this family and they all belong to type 1 RIP genes. This family might have evolved in parallel to species evolution and genome-wide duplications represent the major mechanism for this family expansion. Subsequently, we analyzed their expression under biotic (bacteria and fungus infection), abiotic (cold, drought and salinity) and the phytohormone ABA treatment. These data showed that some members of this family were expressed in various tissues with differentiated expression abundances whereas several members showed no expression under normal growth conditions or various environmental stresses. On the other hand, the expression of many RIP members was regulated by various abiotic and biotic stresses. All these data suggested that specific members of the RIP family in rice might play important roles in biotic and abiotic stress-related biological processes and function as a regulator of various environmental cues and hormone signaling. They may be potentially useful in improving plant tolerance to various abiotic and biotic stresses by over-expressing or suppressing these genes.

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Abbreviations

GUS:

β-glucuronidase

Mg:

Megnaporthe grisea

RIP:

Ribosome-inactivating proteins

Xoo:

Xanthomonas oryzae pv oryzae

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Acknowledgement

We would like to thank Dr. Parameswaran Sriram for his help in real-time PCR analysis.

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Authors and Affiliations

  1. Rice Functional Genomics Group, Temasek Life Sciences Laboratory, 1 Research Link, The National University of Singapore, Singapore, 117604, Singapore

    Shu-Ye Jiang, Rengasamy Ramamoorthy, Ritu Bhalla, Hong-Fen Luan, Prasanna Nori Venkatesh, Minne Cai & Srinivasan Ramachandran

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  1. Shu-Ye Jiang
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  2. Rengasamy Ramamoorthy
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  3. Ritu Bhalla
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  4. Hong-Fen Luan
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  7. Srinivasan Ramachandran
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Correspondence to Srinivasan Ramachandran.

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Jiang, SY., Ramamoorthy, R., Bhalla, R. et al. Genome-wide survey of the RIP domain family in Oryza sativa and their expression profiles under various abiotic and biotic stresses. Plant Mol Biol 67, 603–614 (2008). https://doi.org/10.1007/s11103-008-9342-4

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