Plant Molecular Biology

, Volume 67, Issue 4, pp 375–388 | Cite as

A phosphatidylinositol phosphate-specific myo-inositol polyphosphate 5-phosphatase required for seedling growth

  • Mustafa E. Ercetin
  • Elitsa A. Ananieva
  • Natasha M. Safaee
  • Javad Torabinejad
  • Jamille Y. Robinson
  • Glenda E. Gillaspy


The phosphatidylinositol phosphate signaling pathway is involved in many crucial cellular functions. The myo-inositol polyphosphate 5-phosphatases (5PTases) (E.C. comprise a large protein family that hydrolyze 5-phosphates from a variety of phosphatidylinositol phosphate and inositol phosphate substrates. We previously reported that the At5PTase11 enzyme (At1g47510), which is one of the smallest predicted 5PTases found in any organism, encodes an active 5PTase whose activity is restricted to tris- and bis-, but not mono-phosphorylated phosphatidylinositol phosphate substrates containing a 5-phosphate. This is in contrast to other unrestricted Arabidopsis 5PTases, which also hydrolyze tris- and bis inositol phosphate molecules. To further explore the function of At5PTase11, we have characterized two T-DNA mutants in the At5PTase11 gene, and have complemented this mutant. Seed from 5ptase11 mutants germinate slower than wildtype seed and mutant seedlings have decreased hypocotyl growth as compared to wildtype seedlings when grown in the dark. This phenotype is the opposite of the increased hypocotyl growth phenotype previously described for other 5ptase mutants defective in inositol phosphate-specific 5PTase enzymes. By labeling the endogenous myo-inositol pool in 5ptase11 mutants, we correlated these hypocotyl growth changes with a small increase in the 5PTase11 substrate, phosphatidylinositol (4,5) bisphosphate, and decreases in the potential products of 5PTase11, phosphatidylinositol (3) phosphate and phosphatidylinositol (4) phosphate. Surprisingly, we also found that dark-grown 5ptase11 mutants contain increases in inositol (1,4,5) trisphosphate and an inositol bisphosphate that is not a substrate for recombinant 5PTase11. We present a model for regulation of hypocotyl growth by specific molecules found in this pathway.


Arabidopsis Inositol Phosphoinositide PtdIns(4,5)P2 Ins(1,4,5)P3 





Phosphatidylinositol phosphate


PtdIns(4,5) bisphosphate


Inositol phosphate


Inositol 1,4,5-trisphosphate


Myo-inositol polyphosphate 5-phosphatases




Murashige and Skoog


High performance liquid chromatography



This work is dedicated to the memory of Ryan Christopher Clark. We are grateful to SIGnAL and the Arabidopsis Biological Resource Center for supplying mutant seeds, to Ryan Burnette for assistance in isolating mutants and to Janet Donahue for technical assistance. This work was supported by an award from the USDA (2003-35318-13690) and NSF (MCB0641954) to G.E.G. and by Hatch project (no. VA-135583).

Supplementary material

11103_2008_9327_MOESM1_ESM.tif (33 mb)
Supplemental Fig. 1 Genvestigator expression analysis of At5PTase11. Data from the Genvestigator web site was analyzed and plotted. Note that only NASC data from the 8k array chip and the indicated tissues is available, and that the highest expression level noted (leaf) is only slightly above the general background level specified by Genvestigator (TIF 33823 kb)


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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Mustafa E. Ercetin
    • 1
  • Elitsa A. Ananieva
    • 1
  • Natasha M. Safaee
    • 1
  • Javad Torabinejad
    • 1
  • Jamille Y. Robinson
    • 1
  • Glenda E. Gillaspy
    • 1
  1. 1.Department of BiochemistryVirginia TechBlacksburgUSA

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